The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles
The nucleosome core particle (NCP) is the basic building block of chromatin. Nucleosome-nucleosome interactions are instrumental in chromatin compaction, and understanding NCP self-assembly is important for understanding chromatin structure and dynamics. Recombinant NCPs aggregated by multivalent ca...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/80362 http://hdl.handle.net/10220/40501 |
| _version_ | 1826110514493652992 |
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| author | Berezhnoy, Nikolay V. Liu, Ying Allahverdi, Abdollah Yang, Renliang Su, Chun-Jen Liu, Chuan-Fa Korolev, Nikolay Nordenskiöld, Lars |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Berezhnoy, Nikolay V. Liu, Ying Allahverdi, Abdollah Yang, Renliang Su, Chun-Jen Liu, Chuan-Fa Korolev, Nikolay Nordenskiöld, Lars |
| author_sort | Berezhnoy, Nikolay V. |
| collection | NTU |
| description | The nucleosome core particle (NCP) is the basic building block of chromatin. Nucleosome-nucleosome interactions are instrumental in chromatin compaction, and understanding NCP self-assembly is important for understanding chromatin structure and dynamics. Recombinant NCPs aggregated by multivalent cations form various ordered phases that can be studied by x-ray diffraction (small-angle x-ray scattering). In this work, the effects on the supramolecular structure of aggregated NCPs due to lysine histone H4 tail acetylations, histone H2A mutations (neutralizing the acidic patch of the histone octamer), and the removal of histone tails were investigated. The formation of ordered mainly hexagonal columnar NCP phases is in agreement with earlier studies; however, the highly homogeneous recombinant NCP systems used in this work display a more compact packing. The long-range order of the NCP columnar phase was found to be abolished or reduced by acetylation of the H4 tails, acidic patch neutralization, and removal of the H3 and H2B tails. Loss of nucleosome stacking upon removal of the H3 tails in combination with other tails was observed. In the absence of the H2A tails, the formation of an unknown highly ordered phase was observed. |
| first_indexed | 2024-10-01T02:35:41Z |
| format | Journal Article |
| id | ntu-10356/80362 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:35:41Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/803622023-02-28T16:58:46Z The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles Berezhnoy, Nikolay V. Liu, Ying Allahverdi, Abdollah Yang, Renliang Su, Chun-Jen Liu, Chuan-Fa Korolev, Nikolay Nordenskiöld, Lars School of Biological Sciences Biological Sciences The nucleosome core particle (NCP) is the basic building block of chromatin. Nucleosome-nucleosome interactions are instrumental in chromatin compaction, and understanding NCP self-assembly is important for understanding chromatin structure and dynamics. Recombinant NCPs aggregated by multivalent cations form various ordered phases that can be studied by x-ray diffraction (small-angle x-ray scattering). In this work, the effects on the supramolecular structure of aggregated NCPs due to lysine histone H4 tail acetylations, histone H2A mutations (neutralizing the acidic patch of the histone octamer), and the removal of histone tails were investigated. The formation of ordered mainly hexagonal columnar NCP phases is in agreement with earlier studies; however, the highly homogeneous recombinant NCP systems used in this work display a more compact packing. The long-range order of the NCP columnar phase was found to be abolished or reduced by acetylation of the H4 tails, acidic patch neutralization, and removal of the H3 and H2B tails. Loss of nucleosome stacking upon removal of the H3 tails in combination with other tails was observed. In the absence of the H2A tails, the formation of an unknown highly ordered phase was observed. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Accepted version 2016-05-09T06:17:33Z 2019-12-06T13:47:59Z 2016-05-09T06:17:33Z 2019-12-06T13:47:59Z 2016 2016 Journal Article Berezhnoy, N. V., Liu, Y., Allahverdi, A., Yang, R., Su, C. J., & Liu, C. F. (2016). The Influence of Ionic Environment and Histone Tails on Columnar Order of Nucleosome Core Particles. Biophysical Journal, 110(8), 1720-1731. 0006-3495 https://hdl.handle.net/10356/80362 http://hdl.handle.net/10220/40501 10.1016/j.bpj.2016.03.016 27119633 191776 en Biophysical Journal © 2016 Biophysical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by Biophysical Journal, Biophysical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bpj.2016.03.016]. application/pdf |
| spellingShingle | Biological Sciences Berezhnoy, Nikolay V. Liu, Ying Allahverdi, Abdollah Yang, Renliang Su, Chun-Jen Liu, Chuan-Fa Korolev, Nikolay Nordenskiöld, Lars The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title | The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title_full | The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title_fullStr | The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title_full_unstemmed | The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title_short | The Influence of Ionic Environment and Histone Tails on Ordered Columnar Phases of Nucleosome Core Particles |
| title_sort | influence of ionic environment and histone tails on ordered columnar phases of nucleosome core particles |
| topic | Biological Sciences |
| url | https://hdl.handle.net/10356/80362 http://hdl.handle.net/10220/40501 |
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