Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis
Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/80456 http://hdl.handle.net/10220/46526 |
| _version_ | 1811680164791189504 |
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| author | Gandhimathi, Chinnasamy Praveena, Jayaraman Ramakrishna, Seeram Tham, Allister Yingwei Venugopal, Jayarama Reddy Kumar, Srinivasan Dinesh |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Gandhimathi, Chinnasamy Praveena, Jayaraman Ramakrishna, Seeram Tham, Allister Yingwei Venugopal, Jayarama Reddy Kumar, Srinivasan Dinesh |
| author_sort | Gandhimathi, Chinnasamy |
| collection | NTU |
| description | Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering. |
| first_indexed | 2024-10-01T03:20:42Z |
| format | Journal Article |
| id | ntu-10356/80456 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:20:42Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/804562022-02-16T16:26:28Z Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis Gandhimathi, Chinnasamy Praveena, Jayaraman Ramakrishna, Seeram Tham, Allister Yingwei Venugopal, Jayarama Reddy Kumar, Srinivasan Dinesh Lee Kong Chian School of Medicine (LKCMedicine) Cellular and Molecular Epigenetics Lab Electrospraying Polycaprolactone DRNTU::Science::Medicine Bone transplants are used to treat fractures and increase new tissue development in bone tissue engineering. Grafting of massive implantations showing slow curing rate and results in cell death for poor vascularization. The potentials of biocomposite scaffolds to mimic extracellular matrix (ECM) and including new biomaterials could produce a better substitute for new bone tissue formation. A purpose of this study is to analyze polycaprolactone/silk fibroin/hyaluronic acid/minocycline hydrochloride (PCL/SF/HA/MH) nanoparticles initiate human mesenchymal stem cells (MSCs) proliferation and differentiation into osteogenesis. Electrospraying technique was used to develop PCL, PCL/SF, PCL/SF/HA and PCL/SF/HA/MH hybrid biocomposite nanoparticles and characterization was analyzed by field emission scanning electron microscope (FESEM), contact angle and Fourier transform infrared spectroscopy (FT-IR). The obtained results proved that the particle diameter and water contact angle obtained around 0.54 ± 0.12 to 3.2 ± 0.18 µm and 43.93 ± 10.8° to 133.1 ± 12.4° respectively. The cell proliferation and cell-nanoparticle interactions analyzed using (3-(4,5-dimethyl thiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) MTS assay (Promega, Madison, WI, USA), FESEM for cell morphology and 5-Chloromethylfluorescein diacetate (CMFDA) dye for imaging live cells. Osteogenic differentiation was proved by expression of osteocalcin, alkaline phosphatase activity (ALP) and mineralization was confirmed by using alizarin red (ARS). The quantity of cells was considerably increased in PCL/SF/HA/MH nanoparticles when compare to all other biocomposite nanoparticles and the cell interaction was observed more on PCL/SF/HA/MH nanoparticles. The electrosprayed PCL/SF/HA/MH biocomposite nanoparticle significantly initiated increased cell proliferation, osteogenic differentiation and mineralization, which provide huge potential for bone tissue engineering. MOE (Min. of Education, S’pore) Published version 2018-11-02T02:13:45Z 2019-12-06T13:49:54Z 2018-11-02T02:13:45Z 2019-12-06T13:49:54Z 2016 Journal Article Tham, A. Y., Gandhimathi, C., Praveena, J., Venugopal, J. R., Ramakrishna, S., & Kumar, S. D. (2016). Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis. International Journal of Molecular Sciences, 17(8), 1222-. doi:10.3390/ijms17081222 1661-6596 https://hdl.handle.net/10356/80456 http://hdl.handle.net/10220/46526 10.3390/ijms17081222 27483240 en International Journal of Molecular Sciences © 2016 by The Author(s); licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). 16 p. application/pdf |
| spellingShingle | Electrospraying Polycaprolactone DRNTU::Science::Medicine Gandhimathi, Chinnasamy Praveena, Jayaraman Ramakrishna, Seeram Tham, Allister Yingwei Venugopal, Jayarama Reddy Kumar, Srinivasan Dinesh Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title | Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title_full | Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title_fullStr | Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title_full_unstemmed | Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title_short | Minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| title_sort | minocycline loaded hybrid composites nanoparticles for mesenchymal stem cells differentiation into osteogenesis |
| topic | Electrospraying Polycaprolactone DRNTU::Science::Medicine |
| url | https://hdl.handle.net/10356/80456 http://hdl.handle.net/10220/46526 |
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