The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage
Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Mate...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Online Access: | https://hdl.handle.net/10356/80604 http://hdl.handle.net/10220/40598 |
| _version_ | 1811696391777419264 |
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| author | Nguyen, Luong Thi Hien Liao, Susan Ramakrishna, Seeram Chan, Casey K. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Nguyen, Luong Thi Hien Liao, Susan Ramakrishna, Seeram Chan, Casey K. |
| author_sort | Nguyen, Luong Thi Hien |
| collection | NTU |
| description | Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Materials & Methods: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs. Results & Conclusion: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture. |
| first_indexed | 2024-10-01T07:38:37Z |
| format | Journal Article |
| id | ntu-10356/80604 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:38:37Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/806042020-06-01T10:21:26Z The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage Nguyen, Luong Thi Hien Liao, Susan Ramakrishna, Seeram Chan, Casey K. School of Materials Science & Engineering bone electrospinning mesenchymal stem cells nanofibers osteogenic differentiation serial passage Using scaffolds with autologous stem cells is a golden strategy for the treatment of bone defects. In this strategy, human mesenchymal stem cells (hMSCs) have often been isolated and expanded in vitro on a plastic surface to obtain a sufficient cell number before seeding on a suitable scaffold. Materials & Methods: Investigating the influence of serial passages (from passage two to passage eight) on the abilities of proliferation and osteogenic differentiation of hMSCs on 24-well tissue culture polystyrene plates and poly L-lactic acid electrospun nanofibrous scaffolds was performed to determine how prolonged culture affected these cellular abilities and how the nanofibrous scaffolds supported the osteogenic differentiation potential of hMSCs. Results & Conclusion: Serial passage caused adverse changes in hMSCs characteristics, which were indicated by the decline in both proliferation and osteogenic differentiation abilities. Interestingly, the poly L-lactic acid nanofibrous scaffolds showed a significant support in recovering the osteogenic abilities of hMSCs, which had been severely affected by prolonged culture. NMRC (Natl Medical Research Council, S’pore) 2016-06-02T03:20:58Z 2019-12-06T13:53:05Z 2016-06-02T03:20:58Z 2019-12-06T13:53:05Z 2011 2011 Journal Article Nguyen, L. T. H., Liao, S., Ramakrishna, S., & Chan, C. K. (2011). The role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage. Nanomedicine, 6(6), 961-974. 1743-5889 https://hdl.handle.net/10356/80604 http://hdl.handle.net/10220/40598 10.2217/NNM.11.26 159781 en Nanomedicine © 2011 Future Medicine Ltd. |
| spellingShingle | bone electrospinning mesenchymal stem cells nanofibers osteogenic differentiation serial passage Nguyen, Luong Thi Hien Liao, Susan Ramakrishna, Seeram Chan, Casey K. The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title | The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title_full | The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title_fullStr | The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title_full_unstemmed | The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title_short | The Role of Nanofibrous Structure in Osteogenic Differentiation of Human Mesenchymal Stem Cells with Serial Passage |
| title_sort | role of nanofibrous structure in osteogenic differentiation of human mesenchymal stem cells with serial passage |
| topic | bone electrospinning mesenchymal stem cells nanofibers osteogenic differentiation serial passage |
| url | https://hdl.handle.net/10356/80604 http://hdl.handle.net/10220/40598 |
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