Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis
Mimicking hybrid extracellular matrix is one of the major challenges in bone tissue engineering. Biocomposite micro/nanoparticle of polycaprolactone (PCL), silk fibroin (SF), ascorbic acid (AA) and dexamethasone (DM) were fabricated by the electrospraying methods in order to generate an improved ost...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/80633 http://hdl.handle.net/10220/40616 |
| _version_ | 1811681897737093120 |
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| author | Gandhimathi, Chinnasamy Neo, Xuan Hao Edwin Jayaraman, Praveena Venugopal, Jayarama Reddy Ramakrishna, Seeram Kumar, Srinivasan Dinesh |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Gandhimathi, Chinnasamy Neo, Xuan Hao Edwin Jayaraman, Praveena Venugopal, Jayarama Reddy Ramakrishna, Seeram Kumar, Srinivasan Dinesh |
| author_sort | Gandhimathi, Chinnasamy |
| collection | NTU |
| description | Mimicking hybrid extracellular matrix is one of the major challenges in bone tissue engineering. Biocomposite micro/nanoparticle of polycaprolactone (PCL), silk fibroin (SF), ascorbic acid (AA) and dexamethasone (DM) were fabricated by the electrospraying methods in order to generate an improved osteogenic environment for the proliferation and differentiation of adipose derived stem cells (ADSCs) into osteogenesis. Fabricated electrosprayed micro/nanoparticle was characterized for particle morphology, hydrophilicity, porosity and FTIR analysis for bone tissue regeneration. FESEM micrographs of the nanoparticles revealed porous, fibreless, uniform particles with particle diameter in the range of 720 ± 1.8 nm - 3.5 ± 4.2 µm. The drug release profile indicates that the sustained release of dexamethasone up to 10 days and degradation of nanoparticles around 13-20% after 60 days. ADSCs were cultured on these nanoparticles and were induced to undergo osteogenic differentiation in the presence of AA/DM. The cells morphology, proliferation and interaction were analysed by CMFDA dye extraction method, MTS assay and FESEM analysis respectively.ADSCs differentiation into osteogenesis was confirmed using alkaline phosphatase activity and mineralisation by Alizarin Red staining. The significance of AA and DM biomolecules initiates particular biological functions for the proliferation of ADSCs and differentiation into osteogenic lineages. The obtained results proved that the biocomposite PCL/SF/AA/DM micro/nanoparticle stimulated osteogenic differentiation and mineralisation of ADSCs for bone tissue regeneration. |
| first_indexed | 2024-10-01T03:48:15Z |
| format | Journal Article |
| id | ntu-10356/80633 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:48:15Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/806332020-11-01T05:15:31Z Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis Gandhimathi, Chinnasamy Neo, Xuan Hao Edwin Jayaraman, Praveena Venugopal, Jayarama Reddy Ramakrishna, Seeram Kumar, Srinivasan Dinesh Lee Kong Chian School of Medicine (LKCMedicine) Polycaprolactone Silk fibroin Dexamethasone release Bone tissue engineering Mineralization Mimicking hybrid extracellular matrix is one of the major challenges in bone tissue engineering. Biocomposite micro/nanoparticle of polycaprolactone (PCL), silk fibroin (SF), ascorbic acid (AA) and dexamethasone (DM) were fabricated by the electrospraying methods in order to generate an improved osteogenic environment for the proliferation and differentiation of adipose derived stem cells (ADSCs) into osteogenesis. Fabricated electrosprayed micro/nanoparticle was characterized for particle morphology, hydrophilicity, porosity and FTIR analysis for bone tissue regeneration. FESEM micrographs of the nanoparticles revealed porous, fibreless, uniform particles with particle diameter in the range of 720 ± 1.8 nm - 3.5 ± 4.2 µm. The drug release profile indicates that the sustained release of dexamethasone up to 10 days and degradation of nanoparticles around 13-20% after 60 days. ADSCs were cultured on these nanoparticles and were induced to undergo osteogenic differentiation in the presence of AA/DM. The cells morphology, proliferation and interaction were analysed by CMFDA dye extraction method, MTS assay and FESEM analysis respectively.ADSCs differentiation into osteogenesis was confirmed using alkaline phosphatase activity and mineralisation by Alizarin Red staining. The significance of AA and DM biomolecules initiates particular biological functions for the proliferation of ADSCs and differentiation into osteogenic lineages. The obtained results proved that the biocomposite PCL/SF/AA/DM micro/nanoparticle stimulated osteogenic differentiation and mineralisation of ADSCs for bone tissue regeneration. MOE (Min. of Education, S’pore) Published version 2016-06-06T05:19:11Z 2019-12-06T13:53:35Z 2016-06-06T05:19:11Z 2019-12-06T13:53:35Z 2015 2015 Journal Article Gandhimathi, C., Neo, X. H. E., Jayaraman, P., Venugopal, J. R., Ramakrishna, S., & Kumar, S. D. (2015). Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis. Journal of Drug Metabolism & Toxicology, 6(1), 1000177-. 2157-7609 https://hdl.handle.net/10356/80633 http://hdl.handle.net/10220/40616 10.4172/2157-7609.1000177 185604 en Journal of Drug Metabolism & Toxicology © 2015 The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 7 p. application/pdf |
| spellingShingle | Polycaprolactone Silk fibroin Dexamethasone release Bone tissue engineering Mineralization Gandhimathi, Chinnasamy Neo, Xuan Hao Edwin Jayaraman, Praveena Venugopal, Jayarama Reddy Ramakrishna, Seeram Kumar, Srinivasan Dinesh Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title | Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title_full | Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title_fullStr | Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title_full_unstemmed | Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title_short | Controlled release of dexamethasone in PCL/silk fibroin/ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| title_sort | controlled release of dexamethasone in pcl silk fibroin ascorbic acid nanoparticles for the initiation of adipose derived stem cells into osteogenesis |
| topic | Polycaprolactone Silk fibroin Dexamethasone release Bone tissue engineering Mineralization |
| url | https://hdl.handle.net/10356/80633 http://hdl.handle.net/10220/40616 |
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