Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance
Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated t...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/80926 http://hdl.handle.net/10220/39045 |
| _version_ | 1811679187760578560 |
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| author | Periasamy, Saravanan Nair, Harikrishnan A. S. Lee, Kai Wei Kelvin Ong, Jolene Goh, Jie Q. J. Kjelleberg, Staffan Rice, Scott A. |
| author2 | Interdisciplinary Graduate School (IGS) |
| author_facet | Interdisciplinary Graduate School (IGS) Periasamy, Saravanan Nair, Harikrishnan A. S. Lee, Kai Wei Kelvin Ong, Jolene Goh, Jie Q. J. Kjelleberg, Staffan Rice, Scott A. |
| author_sort | Periasamy, Saravanan |
| collection | NTU |
| description | Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and experimentally informative mixed species biofilm community, consisting of P. aeruginosa, Pseudomonas protegens, and Klebsiella pneumoniae. Loss of the Psl polysaccharide had the biggest impact on the integration of P. aeruginosa in the mixed species biofilms, where the percent composition of the psl mutant was significantly lower (0.06%) than its wild-type (WT) parent (2.44%). In contrast, loss of the Pel polysaccharide had no impact on mixed species biofilm development. Loss of alginate or its overproduction resulted in P. aeruginosa representing 8.4 and 18.11%, respectively, of the mixed species biofilm. Dual species biofilms of P. aeruginosa and K. pneumoniae were not affected by loss of alginate, Pel, or Psl, while the mucoid P. aeruginosa strain achieved a greater biomass than its parent strain. When P. aeruginosa was grown with P. protegens, loss of the Pel or alginate polysaccharides resulted in biofilms that were not significantly different from biofilms formed by the WT PAO1. In contrast, overproduction of alginate resulted in biofilms that were comprised of 35–40% of P. aeruginosa, which was significantly higher than the WT (5–20%). Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%). Loss of the Psl polysaccharide significantly disrupted the communal stress resistance of the three species biofilms. Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities. |
| first_indexed | 2024-10-01T03:05:10Z |
| format | Journal Article |
| id | ntu-10356/80926 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:05:10Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/809262022-02-16T16:29:59Z Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance Periasamy, Saravanan Nair, Harikrishnan A. S. Lee, Kai Wei Kelvin Ong, Jolene Goh, Jie Q. J. Kjelleberg, Staffan Rice, Scott A. Interdisciplinary Graduate School (IGS) School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Exopolysaccharides Biofilms Mixedspeciesconsortia Interspeciescompetition Stresstolerance Pseudomonas aeruginosa PAO1 produces three polysaccharides, alginate, Psl, and Pel that play distinct roles in attachment and biofilm formation for monospecies biofilms. Considerably less is known about their role in the development of mixed species biofilm communities. This study has investigated the roles of alginate, Psl, and Pel during biofilm formation of P. aeruginosa in a defined and experimentally informative mixed species biofilm community, consisting of P. aeruginosa, Pseudomonas protegens, and Klebsiella pneumoniae. Loss of the Psl polysaccharide had the biggest impact on the integration of P. aeruginosa in the mixed species biofilms, where the percent composition of the psl mutant was significantly lower (0.06%) than its wild-type (WT) parent (2.44%). In contrast, loss of the Pel polysaccharide had no impact on mixed species biofilm development. Loss of alginate or its overproduction resulted in P. aeruginosa representing 8.4 and 18.11%, respectively, of the mixed species biofilm. Dual species biofilms of P. aeruginosa and K. pneumoniae were not affected by loss of alginate, Pel, or Psl, while the mucoid P. aeruginosa strain achieved a greater biomass than its parent strain. When P. aeruginosa was grown with P. protegens, loss of the Pel or alginate polysaccharides resulted in biofilms that were not significantly different from biofilms formed by the WT PAO1. In contrast, overproduction of alginate resulted in biofilms that were comprised of 35–40% of P. aeruginosa, which was significantly higher than the WT (5–20%). Loss of the Psl polysaccharide significantly reduced the percentage composition of P. aeruginosa in dual species biofilms with P. protegens (<1%). Loss of the Psl polysaccharide significantly disrupted the communal stress resistance of the three species biofilms. Thus, the polysaccharide composition of an individual species significantly impacts mixed species biofilm development and the emergent properties of such communities. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Published version 2015-12-10T09:29:19Z 2019-12-06T14:17:34Z 2015-12-10T09:29:19Z 2019-12-06T14:17:34Z 2015 Journal Article Periasamy, S., Nair, H. A. S., Lee, K. W. K., Ong, J., Goh, J. Q. J., Kjelleberg, S., et al. (2015). Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance. Frontiers in Microbiology, 6, 851-. 1664-302X https://hdl.handle.net/10356/80926 http://hdl.handle.net/10220/39045 10.3389/fmicb.2015.00851 26347731 en Frontiers in Microbiology © 2015 Periasamy, Nair, Lee, Ong, Goh, Kjelleberg and Rice. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 10 p. application/pdf |
| spellingShingle | Exopolysaccharides Biofilms Mixedspeciesconsortia Interspeciescompetition Stresstolerance Periasamy, Saravanan Nair, Harikrishnan A. S. Lee, Kai Wei Kelvin Ong, Jolene Goh, Jie Q. J. Kjelleberg, Staffan Rice, Scott A. Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title | Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title_full | Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title_fullStr | Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title_full_unstemmed | Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title_short | Pseudomonas aeruginosa PAO1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| title_sort | pseudomonas aeruginosa pao1 exopolysaccharides are important for mixed species biofilm community development and stress tolerance |
| topic | Exopolysaccharides Biofilms Mixedspeciesconsortia Interspeciescompetition Stresstolerance |
| url | https://hdl.handle.net/10356/80926 http://hdl.handle.net/10220/39045 |
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