Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide
Using total internal fluorescence microscopy, highly parallel measurements of single lipid vesicles unexpectedly reveal that a small fraction of vesicles rupture in multiple discrete steps when destabilized by a membrane-active peptide which is in contrast to classical solubilization models. To acco...
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| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/80927 http://hdl.handle.net/10220/40633 |
| _version_ | 1826113306370244608 |
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| author | Tabaei, Seyed Ruhollah Cho, Nam-Joon |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Tabaei, Seyed Ruhollah Cho, Nam-Joon |
| author_sort | Tabaei, Seyed Ruhollah |
| collection | NTU |
| description | Using total internal fluorescence microscopy, highly parallel measurements of single lipid vesicles unexpectedly reveal that a small fraction of vesicles rupture in multiple discrete steps when destabilized by a membrane-active peptide which is in contrast to classical solubilization models. To account for this surprizing kinetic behaviour, we identified that this vesicle subpopulation consists of multilamellar vesicles, and that the outermost lamella is more susceptible to rupture than unilamellar vesicles of even smaller size. This finding sheds light on the multiple ways in which membrane configuration can influence strain in the bilayer leaflet and contribute to nm-scale membrane curvature sensing. |
| first_indexed | 2024-10-01T03:21:03Z |
| format | Journal Article |
| id | ntu-10356/80927 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:21:03Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/809272020-06-01T10:13:50Z Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide Tabaei, Seyed Ruhollah Cho, Nam-Joon School of Materials Science & Engineering School of Chemical and Biomedical Engineering Centre for Biomimetic Sensor Science Nanostructures Using total internal fluorescence microscopy, highly parallel measurements of single lipid vesicles unexpectedly reveal that a small fraction of vesicles rupture in multiple discrete steps when destabilized by a membrane-active peptide which is in contrast to classical solubilization models. To account for this surprizing kinetic behaviour, we identified that this vesicle subpopulation consists of multilamellar vesicles, and that the outermost lamella is more susceptible to rupture than unilamellar vesicles of even smaller size. This finding sheds light on the multiple ways in which membrane configuration can influence strain in the bilayer leaflet and contribute to nm-scale membrane curvature sensing. NRF (Natl Research Foundation, S’pore) NMRC (Natl Medical Research Council, S’pore) 2016-06-08T03:28:20Z 2019-12-06T14:17:34Z 2016-06-08T03:28:20Z 2019-12-06T14:17:34Z 2015 Journal Article Tabaei, S. R., & Cho, N. -J. (2015). Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide. Chemical Communications, 51, 10272-10275. 1359-7345 https://hdl.handle.net/10356/80927 http://hdl.handle.net/10220/40633 10.1039/C5CC02769A en Chemical Communications © 2015 The Royal Society of Chemistry. |
| spellingShingle | Nanostructures Tabaei, Seyed Ruhollah Cho, Nam-Joon Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title | Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title_full | Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title_fullStr | Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title_full_unstemmed | Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title_short | Lamellar sheet exfoliation of single lipid vesicles by a membrane-active peptide |
| title_sort | lamellar sheet exfoliation of single lipid vesicles by a membrane active peptide |
| topic | Nanostructures |
| url | https://hdl.handle.net/10356/80927 http://hdl.handle.net/10220/40633 |
| work_keys_str_mv | AT tabaeiseyedruhollah lamellarsheetexfoliationofsinglelipidvesiclesbyamembraneactivepeptide AT chonamjoon lamellarsheetexfoliationofsinglelipidvesiclesbyamembraneactivepeptide |