Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability
The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure...
| Main Authors: | , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/80934 http://hdl.handle.net/10220/38991 |
| _version_ | 1811695529859481600 |
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| author | Mundargi, Raghavendra Venkataraman, Divya Kumar, Saranya Mogal, Vishal Ortiz, Raphael Steele, Terry W. J. Loo, Joachim Say Chye Venkatraman, Subbu S. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Mundargi, Raghavendra Venkataraman, Divya Kumar, Saranya Mogal, Vishal Ortiz, Raphael Steele, Terry W. J. Loo, Joachim Say Chye Venkatraman, Subbu S. |
| author_sort | Mundargi, Raghavendra |
| collection | NTU |
| description | The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator “resorufin.” Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson’s trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices. |
| first_indexed | 2024-10-01T07:24:55Z |
| format | Journal Article |
| id | ntu-10356/80934 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:24:55Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/809342023-07-14T15:49:30Z Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability Mundargi, Raghavendra Venkataraman, Divya Kumar, Saranya Mogal, Vishal Ortiz, Raphael Steele, Terry W. J. Loo, Joachim Say Chye Venkatraman, Subbu S. School of Materials Science & Engineering The aim of the present work is to design and construct an ex vivo bioreactor system to assess the real time viability of vascular tissue. Porcine carotid artery as a model tissue was used in the ex vivo bioreactor setup to monitor its viability under physiological conditions such as oxygen, pressure, temperature, and flow. The real time tissue viability was evaluated by monitoring tissue metabolism through a fluorescent indicator “resorufin.” Our ex vivo bioreactor allows real time monitoring of tissue responses along with physiological conditions. These ex vivo parameters were vital in determining the tissue viability in sensor-enabled bioreactor and our initial investigations suggest that, porcine tissue viability is considerably affected by high shear forces and low oxygen levels. Histological evaluations with hematoxylin and eosin and Masson’s trichrome staining show intact endothelium with fresh porcine tissue whereas tissues after incubation in ex vivo bioreactor studies indicate denuded endothelium supporting the viability results from real time measurements. Hence, this novel viability sensor-enabled ex vivo bioreactor acts as model to mimic in vivo system and record vascular responses to biopharmaceutical molecules and biomedical devices. Published version 2015-12-08T02:28:07Z 2019-12-06T14:17:45Z 2015-12-08T02:28:07Z 2019-12-06T14:17:45Z 2015 Journal Article Mundargi, R., Venkataraman, D., Kumar, S., Mogal, V., Ortiz, R., Loo, J. S. C., et al (2015). Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability. BioMed Research International, 2015, 958170-. 2314-6133 https://hdl.handle.net/10356/80934 http://hdl.handle.net/10220/38991 10.1155/2015/958170 26609536 en BioMed Research International © 2015 Raghavendra Mundargi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 8 p. application/pdf |
| spellingShingle | Mundargi, Raghavendra Venkataraman, Divya Kumar, Saranya Mogal, Vishal Ortiz, Raphael Steele, Terry W. J. Loo, Joachim Say Chye Venkatraman, Subbu S. Novel Sensor-Enabled Ex Vivo Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title | Novel Sensor-Enabled
Ex Vivo
Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title_full | Novel Sensor-Enabled
Ex Vivo
Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title_fullStr | Novel Sensor-Enabled
Ex Vivo
Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title_full_unstemmed | Novel Sensor-Enabled
Ex Vivo
Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title_short | Novel Sensor-Enabled
Ex Vivo
Bioreactor: A New Approach towards Physiological Parameters and Porcine Artery Viability |
| title_sort | novel sensor enabled ex vivo bioreactor a new approach towards physiological parameters and porcine artery viability |
| url | https://hdl.handle.net/10356/80934 http://hdl.handle.net/10220/38991 |
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