Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection
The application of microfluidic devices in diagnostic systems is well-established in contemporary research. Large specific surface area of microspheres, on the other hand, has secured an important position for their use in bioanalytical assays. Herein, we report a combination of microspheres and mic...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/80984 http://hdl.handle.net/10220/38983 |
| _version_ | 1811696741663113216 |
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| author | Hosseini, Samira Aeinehvand, Mohammad M. Uddin, Shah M. Benzina, Abderazak Rothan, Hussin A. Yusof, Rohana Koole, Leo H. Madou, Marc J. Djordjevic, Ivan Ibrahim, Fatimah |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Hosseini, Samira Aeinehvand, Mohammad M. Uddin, Shah M. Benzina, Abderazak Rothan, Hussin A. Yusof, Rohana Koole, Leo H. Madou, Marc J. Djordjevic, Ivan Ibrahim, Fatimah |
| author_sort | Hosseini, Samira |
| collection | NTU |
| description | The application of microfluidic devices in diagnostic systems is well-established in contemporary research. Large specific surface area of microspheres, on the other hand, has secured an important position for their use in bioanalytical assays. Herein, we report a combination of microspheres and microfluidic disk in a unique hybrid platform for highly sensitive and selective detection of dengue virus. Surface engineered polymethacrylate microspheres with carefully designed functional groups facilitate biorecognition in a multitude manner. In order to maximize the utility of the microspheres’ specific surface area in biomolecular interaction, the microfluidic disk was equipped with a micromixing system. The mixing mechanism (microballoon mixing) enhances the number of molecular encounters between spheres and target analyte by accessing the entire sample volume more effectively, which subsequently results in signal amplification. Significant reduction of incubation time along with considerable lower detection limits were the prime motivations for the integration of microspheres inside the microfluidic disk. Lengthy incubations of routine analytical assays were reduced from 2 hours to 5 minutes while developed system successfully detected a few units of dengue virus. Obtained results make this hybrid microsphere-microfluidic approach to dengue detection a promising avenue for early detection of this fatal illness. |
| first_indexed | 2024-10-01T07:44:11Z |
| format | Journal Article |
| id | ntu-10356/80984 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:44:11Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/809842023-07-14T15:50:03Z Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection Hosseini, Samira Aeinehvand, Mohammad M. Uddin, Shah M. Benzina, Abderazak Rothan, Hussin A. Yusof, Rohana Koole, Leo H. Madou, Marc J. Djordjevic, Ivan Ibrahim, Fatimah School of Materials Science & Engineering The application of microfluidic devices in diagnostic systems is well-established in contemporary research. Large specific surface area of microspheres, on the other hand, has secured an important position for their use in bioanalytical assays. Herein, we report a combination of microspheres and microfluidic disk in a unique hybrid platform for highly sensitive and selective detection of dengue virus. Surface engineered polymethacrylate microspheres with carefully designed functional groups facilitate biorecognition in a multitude manner. In order to maximize the utility of the microspheres’ specific surface area in biomolecular interaction, the microfluidic disk was equipped with a micromixing system. The mixing mechanism (microballoon mixing) enhances the number of molecular encounters between spheres and target analyte by accessing the entire sample volume more effectively, which subsequently results in signal amplification. Significant reduction of incubation time along with considerable lower detection limits were the prime motivations for the integration of microspheres inside the microfluidic disk. Lengthy incubations of routine analytical assays were reduced from 2 hours to 5 minutes while developed system successfully detected a few units of dengue virus. Obtained results make this hybrid microsphere-microfluidic approach to dengue detection a promising avenue for early detection of this fatal illness. Published version 2015-12-07T08:34:08Z 2019-12-06T14:18:54Z 2015-12-07T08:34:08Z 2019-12-06T14:18:54Z 2015 Journal Article Hosseini, S., Aeinehvand, M. M., Uddin, S. M., Benzina, A., Rothan, H. A., Yusof, R., et al. Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection. Scientific Reports, 5, 16485-. 2045-2322 https://hdl.handle.net/10356/80984 http://hdl.handle.net/10220/38983 10.1038/srep16485 26548806 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 14 p. application/pdf |
| spellingShingle | Hosseini, Samira Aeinehvand, Mohammad M. Uddin, Shah M. Benzina, Abderazak Rothan, Hussin A. Yusof, Rohana Koole, Leo H. Madou, Marc J. Djordjevic, Ivan Ibrahim, Fatimah Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title | Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title_full | Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title_fullStr | Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title_full_unstemmed | Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title_short | Microsphere integrated microfluidic disk: synergy of two techniques for rapid and ultrasensitive dengue detection |
| title_sort | microsphere integrated microfluidic disk synergy of two techniques for rapid and ultrasensitive dengue detection |
| url | https://hdl.handle.net/10356/80984 http://hdl.handle.net/10220/38983 |
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