An excitatory GABA loop operating in vivo
While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-phot...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/81000 http://hdl.handle.net/10220/39040 |
| _version_ | 1826120172674482176 |
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| author | Astorga, Guadalupe Bao, Jin Marty, Alain Augustine, George James Franconville, Romain Jalil, Abdelali Bradley, Jonathan Llano, Isabel |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Astorga, Guadalupe Bao, Jin Marty, Alain Augustine, George James Franconville, Romain Jalil, Abdelali Bradley, Jonathan Llano, Isabel |
| author_sort | Astorga, Guadalupe |
| collection | NTU |
| description | While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-photon calcium imaging in vivo, we find that activation of chloride-permeable GABAA receptors in parallel fibers (PFs) of the cerebellar molecular layer of adult mice causes parallel fiber excitation. Stimulation of PFs at submaximal stimulus intensities leads to GABA release from molecular layer interneurons (MLIs), thus creating a positive feedback loop that enhances excitation near the center of an activated PF bundle. Our results imply that elevated chloride concentration can occur in specific intracellular compartments of mature mammalian neurons and suggest an excitatory role for GABAA receptors in the cerebellar cortex of adult mice. |
| first_indexed | 2024-10-01T05:12:09Z |
| format | Journal Article |
| id | ntu-10356/81000 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:12:09Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/810002022-02-16T16:31:22Z An excitatory GABA loop operating in vivo Astorga, Guadalupe Bao, Jin Marty, Alain Augustine, George James Franconville, Romain Jalil, Abdelali Bradley, Jonathan Llano, Isabel Lee Kong Chian School of Medicine (LKCMedicine) GABA Interneurons Calcium Cerebellum Parallel fibers While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in the mammalian brain, much remains to be learned concerning the circumstances and the cellular mechanisms governing potential excitatory GABA action. Using a combination of optogenetics and two-photon calcium imaging in vivo, we find that activation of chloride-permeable GABAA receptors in parallel fibers (PFs) of the cerebellar molecular layer of adult mice causes parallel fiber excitation. Stimulation of PFs at submaximal stimulus intensities leads to GABA release from molecular layer interneurons (MLIs), thus creating a positive feedback loop that enhances excitation near the center of an activated PF bundle. Our results imply that elevated chloride concentration can occur in specific intracellular compartments of mature mammalian neurons and suggest an excitatory role for GABAA receptors in the cerebellar cortex of adult mice. Published version 2015-12-10T08:16:23Z 2019-12-06T14:19:16Z 2015-12-10T08:16:23Z 2019-12-06T14:19:16Z 2015 Journal Article Astorga, G., Bao, J., Marty, A., Augustine, G. J., Franconville, R., Jalil, A., et al. (2015). An excitatory GABA loop operating in vivo. Frontiers in Cellular Neuroscience, 9, 275-. 1662-5102 https://hdl.handle.net/10356/81000 http://hdl.handle.net/10220/39040 10.3389/fncel.2015.00275 26236197 en Frontiers in Cellular Neuroscience © 2015 Astorga, Bao, Marty, Augustine, Franconville, Jalil, Bradley and Llano. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 14 p. application/pdf |
| spellingShingle | GABA Interneurons Calcium Cerebellum Parallel fibers Astorga, Guadalupe Bao, Jin Marty, Alain Augustine, George James Franconville, Romain Jalil, Abdelali Bradley, Jonathan Llano, Isabel An excitatory GABA loop operating in vivo |
| title | An excitatory GABA loop operating in vivo |
| title_full | An excitatory GABA loop operating in vivo |
| title_fullStr | An excitatory GABA loop operating in vivo |
| title_full_unstemmed | An excitatory GABA loop operating in vivo |
| title_short | An excitatory GABA loop operating in vivo |
| title_sort | excitatory gaba loop operating in vivo |
| topic | GABA Interneurons Calcium Cerebellum Parallel fibers |
| url | https://hdl.handle.net/10356/81000 http://hdl.handle.net/10220/39040 |
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