Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica
Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greate...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/81036 http://hdl.handle.net/10220/40654 |
| _version_ | 1826111468933742592 |
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| author | Wallin, Maria Choi, Jae‑Hyeok Kim, Seong Oh Cho, Nam‑Joon Andersson, Martin |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Wallin, Maria Choi, Jae‑Hyeok Kim, Seong Oh Cho, Nam‑Joon Andersson, Martin |
| author_sort | Wallin, Maria |
| collection | NTU |
| description | Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greater stability. The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol % 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. A quartz crystal microbalance with dissipation monitoring was used to monitor the process of vesicle adsorption and tBLM self-assembly, and atomic force microscopy was performed to characterize the structural properties of the tBLM obtained. Whereas tether-containing lipid vesicles ruptured neither spontaneously nor as a result of osmotic shock, introduction of an amphipathic α-helical (AH) peptide induced vesicle rupture and subsequent tBLM formation. Taken together, our findings suggest that the AH peptide is an efficient means of rupturing vesicles of both simple and complex composition, and is, therefore, useful for formation of tBLMs on solid and mesoporous materials for applications in biotechnology. |
| first_indexed | 2024-10-01T02:51:13Z |
| format | Journal Article |
| id | ntu-10356/81036 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:51:13Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/810362020-06-01T10:21:15Z Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica Wallin, Maria Choi, Jae‑Hyeok Kim, Seong Oh Cho, Nam‑Joon Andersson, Martin School of Chemical and Biomedical Engineering School of Materials Science & Engineering Mesoporous Tethered lipid bilayer membrane AH peptide Vesicle fusion Tethered bilayer lipid membranes (tBLMs) on solid supports have substantial advantages as models of artificial cell membranes for such biomedical applications as drug delivery and biosensing. Compared with untethered lipid membranes, tBLMs have more space between substrate and the bilayer and greater stability. The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol % 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. A quartz crystal microbalance with dissipation monitoring was used to monitor the process of vesicle adsorption and tBLM self-assembly, and atomic force microscopy was performed to characterize the structural properties of the tBLM obtained. Whereas tether-containing lipid vesicles ruptured neither spontaneously nor as a result of osmotic shock, introduction of an amphipathic α-helical (AH) peptide induced vesicle rupture and subsequent tBLM formation. Taken together, our findings suggest that the AH peptide is an efficient means of rupturing vesicles of both simple and complex composition, and is, therefore, useful for formation of tBLMs on solid and mesoporous materials for applications in biotechnology. NRF (Natl Research Foundation, S’pore) NMRC (Natl Medical Research Council, S’pore) 2016-06-09T05:45:40Z 2019-12-06T14:20:02Z 2016-06-09T05:45:40Z 2019-12-06T14:20:02Z 2014 Journal Article Wallin, M., Choi, J.-H., Kim, S. O., Cho, N.-J., & Andersson, M. (2015). Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica. European Biophysics Journal, 44(1), 27-36. 0175-7571 https://hdl.handle.net/10356/81036 http://hdl.handle.net/10220/40654 10.1007/s00249-014-0998-1 en European Biophysics Journal © 2014 European Biophysical Societies’ Association |
| spellingShingle | Mesoporous Tethered lipid bilayer membrane AH peptide Vesicle fusion Wallin, Maria Choi, Jae‑Hyeok Kim, Seong Oh Cho, Nam‑Joon Andersson, Martin Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title | Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title_full | Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title_fullStr | Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title_full_unstemmed | Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title_short | Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| title_sort | peptide induced formation of a tethered lipid bilayer membrane on mesoporous silica |
| topic | Mesoporous Tethered lipid bilayer membrane AH peptide Vesicle fusion |
| url | https://hdl.handle.net/10356/81036 http://hdl.handle.net/10220/40654 |
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