Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models
Traditional cancer treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to tumor hypoxia. While bacterial cancer therapy has the potential to overcome this problem, it comes with the risk of toxicity and infection. To circumvent these issues, this paper invest...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2015
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| Online Access: | https://hdl.handle.net/10356/81057 http://hdl.handle.net/10220/39058 |
| _version_ | 1826122620470296576 |
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| author | Bhave, Madhura Satish Hassanbhai, Ammar Mansoor Anand, Padmaja Luo, Kathy Qian Teoh, Swee Hin |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Bhave, Madhura Satish Hassanbhai, Ammar Mansoor Anand, Padmaja Luo, Kathy Qian Teoh, Swee Hin |
| author_sort | Bhave, Madhura Satish |
| collection | NTU |
| description | Traditional cancer treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to tumor hypoxia. While bacterial cancer therapy has the potential to overcome this problem, it comes with the risk of toxicity and infection. To circumvent these issues, this paper investigates the anti-tumor effects of non-viable bacterial derivatives of Clostridium sporogenes. These non-viable derivatives are heat-inactivated C. sporogenes bacteria (IB) and the secreted bacterial proteins in culture media, known as conditioned media (CM). In this project, the effects of IB and CM on CT26 and HCT116 colorectal cancer cells were examined on a 2-Dimensional (2D) and 3-Dimensional (3D) platform. IB significantly inhibited cell proliferation of CT26 to 6.3% of the control in 72 hours for the 2D monolayer culture. In the 3D spheroid culture, cell proliferation of HCT116 spheroids notably dropped to 26.2%. Similarly the CM also remarkably reduced the cell-proliferation of the CT26 cells to 2.4% and 20% in the 2D and 3D models, respectively. Interestingly the effect of boiled conditioned media (BCM) on the cells in the 3D model was less inhibitory than that of CM. Thus, the inhibitive effect of inactivated C. sporogenes and its conditioned media on colorectal cancer cells is established. |
| first_indexed | 2024-10-01T05:51:08Z |
| format | Journal Article |
| id | ntu-10356/81057 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:51:08Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/810572023-12-29T06:49:54Z Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models Bhave, Madhura Satish Hassanbhai, Ammar Mansoor Anand, Padmaja Luo, Kathy Qian Teoh, Swee Hin School of Chemical and Biomedical Engineering Traditional cancer treatments, such as chemotherapy and radiation therapy continue to have limited efficacy due to tumor hypoxia. While bacterial cancer therapy has the potential to overcome this problem, it comes with the risk of toxicity and infection. To circumvent these issues, this paper investigates the anti-tumor effects of non-viable bacterial derivatives of Clostridium sporogenes. These non-viable derivatives are heat-inactivated C. sporogenes bacteria (IB) and the secreted bacterial proteins in culture media, known as conditioned media (CM). In this project, the effects of IB and CM on CT26 and HCT116 colorectal cancer cells were examined on a 2-Dimensional (2D) and 3-Dimensional (3D) platform. IB significantly inhibited cell proliferation of CT26 to 6.3% of the control in 72 hours for the 2D monolayer culture. In the 3D spheroid culture, cell proliferation of HCT116 spheroids notably dropped to 26.2%. Similarly the CM also remarkably reduced the cell-proliferation of the CT26 cells to 2.4% and 20% in the 2D and 3D models, respectively. Interestingly the effect of boiled conditioned media (BCM) on the cells in the 3D model was less inhibitory than that of CM. Thus, the inhibitive effect of inactivated C. sporogenes and its conditioned media on colorectal cancer cells is established. Published version 2015-12-14T01:18:28Z 2019-12-06T14:20:31Z 2015-12-14T01:18:28Z 2019-12-06T14:20:31Z 2015 Journal Article Bhave, M. S., Hassanbhai, A. M., Anand, P., Luo, K. Q., & Teoh, S. H. (2015). Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models. Scientific Reports, 5, 15681-. 2045-2322 https://hdl.handle.net/10356/81057 http://hdl.handle.net/10220/39058 10.1038/srep15681 26507312 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 12 p. application/pdf |
| spellingShingle | Bhave, Madhura Satish Hassanbhai, Ammar Mansoor Anand, Padmaja Luo, Kathy Qian Teoh, Swee Hin Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title | Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title_full | Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title_fullStr | Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title_full_unstemmed | Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title_short | Effect of Heat-Inactivated Clostridium sporogenes and Its Conditioned Media on 3-Dimensional Colorectal Cancer Cell Models |
| title_sort | effect of heat inactivated clostridium sporogenes and its conditioned media on 3 dimensional colorectal cancer cell models |
| url | https://hdl.handle.net/10356/81057 http://hdl.handle.net/10220/39058 |
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