Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human
Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of...
| Main Authors: | , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
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| Online Access: | https://hdl.handle.net/10356/81095 http://hdl.handle.net/10220/39052 |
| _version_ | 1826129417451077632 |
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| author | Chen, Qingfeng Ye, Weijian Liu, Min Tan, Shu Qi Loh, Eva Chang, Kenneth T. E. Tan, Thiam Chye Preiser, Peter R. Tan, Wei Jian Yong, Kylie Su Mei Chen, Jianzhu |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Chen, Qingfeng Ye, Weijian Liu, Min Tan, Shu Qi Loh, Eva Chang, Kenneth T. E. Tan, Thiam Chye Preiser, Peter R. Tan, Wei Jian Yong, Kylie Su Mei Chen, Jianzhu |
| author_sort | Chen, Qingfeng |
| collection | NTU |
| description | Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of a cell population in the bone marrow of the cytokine-treated humanized mice that express both NK cell marker CD56 and myeloid markers such as CD36 and CD33. The CD56+CD33+CD36+ cells are also found in human cord blood, fetal and adult bone marrow. Although the CD56+CD33+CD36+ cells do not express the common NK cell functional receptors and exhibit little cytotoxic and cytokine-producing activities, they readily differentiate into mature NK cells by acquiring expression of NK cell receptors and losing expression of the myeloid markers. Further studies show that CD33+CD36+ myeloid NK precursors are derived from granulo-myelomonocytic progenitors. These results delineate the pathway of human NK cell differentiation from myeloid progenitors in the bone marrow and suggest the utility of humanized mice for studying human hematopoiesis. |
| first_indexed | 2024-10-01T07:40:15Z |
| format | Journal Article |
| id | ntu-10356/81095 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:40:15Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/810952023-02-28T16:58:18Z Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human Chen, Qingfeng Ye, Weijian Liu, Min Tan, Shu Qi Loh, Eva Chang, Kenneth T. E. Tan, Thiam Chye Preiser, Peter R. Tan, Wei Jian Yong, Kylie Su Mei Chen, Jianzhu School of Biological Sciences Understanding of natural killer (NK) cell development in human is incomplete partly because of limited access to appropriate human tissues. We have developed a cytokine-enhanced humanized mouse model with greatly improved reconstitution and function of human NK cells. Here we report the presence of a cell population in the bone marrow of the cytokine-treated humanized mice that express both NK cell marker CD56 and myeloid markers such as CD36 and CD33. The CD56+CD33+CD36+ cells are also found in human cord blood, fetal and adult bone marrow. Although the CD56+CD33+CD36+ cells do not express the common NK cell functional receptors and exhibit little cytotoxic and cytokine-producing activities, they readily differentiate into mature NK cells by acquiring expression of NK cell receptors and losing expression of the myeloid markers. Further studies show that CD33+CD36+ myeloid NK precursors are derived from granulo-myelomonocytic progenitors. These results delineate the pathway of human NK cell differentiation from myeloid progenitors in the bone marrow and suggest the utility of humanized mice for studying human hematopoiesis. Published version 2015-12-11T08:14:31Z 2019-12-06T14:21:18Z 2015-12-11T08:14:31Z 2019-12-06T14:21:18Z 2015 Journal Article Chen, Q., Ye, W., Tan, W. J., Yong, K. S. M., Liu, M., Tan, S. Q., et al. (2015). Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human. Scientific Reports, 5, 15118-. 2045-2322 https://hdl.handle.net/10356/81095 http://hdl.handle.net/10220/39052 10.1038/srep15118 26456148 en Scientific Reports This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 12 p. application/pdf |
| spellingShingle | Chen, Qingfeng Ye, Weijian Liu, Min Tan, Shu Qi Loh, Eva Chang, Kenneth T. E. Tan, Thiam Chye Preiser, Peter R. Tan, Wei Jian Yong, Kylie Su Mei Chen, Jianzhu Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_full | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_fullStr | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_full_unstemmed | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_short | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human |
| title_sort | delineation of natural killer cell differentiation from myeloid progenitors in human |
| url | https://hdl.handle.net/10356/81095 http://hdl.handle.net/10220/39052 |
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