Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection
The Type Six Secretion System (T6SS) is required for Bordetella bronchiseptica cytotoxicity, cytokine modulation, infection, and persistence. However, one-third of recently sequenced Bordetella bronchiseptica strains of the predominantly human-associated Complex IV have lost their T6SS through gene...
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/81318 http://hdl.handle.net/10220/39220 |
| _version_ | 1826118101867954176 |
|---|---|
| author | Bendor, Liron Weyrich, Laura S. Linz, Bodo Rolin, Olivier Y. Taylor, Dawn L. Goodfield, Laura L. Smallridge, William E. Kennett, Mary J. Harvill, Eric Thomas |
| author2 | Jeyaseelan, Samithamby |
| author_facet | Jeyaseelan, Samithamby Bendor, Liron Weyrich, Laura S. Linz, Bodo Rolin, Olivier Y. Taylor, Dawn L. Goodfield, Laura L. Smallridge, William E. Kennett, Mary J. Harvill, Eric Thomas |
| author_sort | Bendor, Liron |
| collection | NTU |
| description | The Type Six Secretion System (T6SS) is required for Bordetella bronchiseptica cytotoxicity, cytokine modulation, infection, and persistence. However, one-third of recently sequenced Bordetella bronchiseptica strains of the predominantly human-associated Complex IV have lost their T6SS through gene deletion or degradation. Since most human B. bronchiseptica infections occur in immunocompromised patients, we determine here whether loss of Type Six Secretion is beneficial to B. bronchiseptica during infection of immunocompromised mice. Infection of mice lacking adaptive immunity (Rag1-/- mice) with a T6SS-deficient mutant results in a hypervirulent phenotype that is characterized by high numbers of intracellular bacteria in systemic organs. In contrast, wild-type B. bronchiseptica kill their eukaryotic cellular hosts via a T6SS-dependent mechanism that prevents survival in systemic organs. High numbers of intracellular bacteria recovered from immunodeficient mice but only low numbers from wild-type mice demonstrates that B. bronchiseptica survival in an intracellular niche is limited by B and T cell responses. Understanding the nature of intracellular survival during infection, and its effects on the generation and function of the host immune response, are important to contain and control the spread of Bordetella-caused disease. |
| first_indexed | 2024-10-01T04:38:14Z |
| format | Journal Article |
| id | ntu-10356/81318 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:38:14Z |
| publishDate | 2015 |
| record_format | dspace |
| spelling | ntu-10356/813182022-02-16T16:26:44Z Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection Bendor, Liron Weyrich, Laura S. Linz, Bodo Rolin, Olivier Y. Taylor, Dawn L. Goodfield, Laura L. Smallridge, William E. Kennett, Mary J. Harvill, Eric Thomas Jeyaseelan, Samithamby Lee Kong Chian School of Medicine (LKCMedicine) Singapore Centre for Environmental Life Sciences Engineering Medicine The Type Six Secretion System (T6SS) is required for Bordetella bronchiseptica cytotoxicity, cytokine modulation, infection, and persistence. However, one-third of recently sequenced Bordetella bronchiseptica strains of the predominantly human-associated Complex IV have lost their T6SS through gene deletion or degradation. Since most human B. bronchiseptica infections occur in immunocompromised patients, we determine here whether loss of Type Six Secretion is beneficial to B. bronchiseptica during infection of immunocompromised mice. Infection of mice lacking adaptive immunity (Rag1-/- mice) with a T6SS-deficient mutant results in a hypervirulent phenotype that is characterized by high numbers of intracellular bacteria in systemic organs. In contrast, wild-type B. bronchiseptica kill their eukaryotic cellular hosts via a T6SS-dependent mechanism that prevents survival in systemic organs. High numbers of intracellular bacteria recovered from immunodeficient mice but only low numbers from wild-type mice demonstrates that B. bronchiseptica survival in an intracellular niche is limited by B and T cell responses. Understanding the nature of intracellular survival during infection, and its effects on the generation and function of the host immune response, are important to contain and control the spread of Bordetella-caused disease. Published version 2015-12-23T08:36:04Z 2019-12-06T14:28:14Z 2015-12-23T08:36:04Z 2019-12-06T14:28:14Z 2015 Journal Article Bendor, L., Weyrich, L. S., Linz, B., Rolin, O. Y., Taylor, D. L., Goodfield, L. L., et al. (2015). Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection. PLOS ONE, 10(10), e0140743-. 1932-6203 https://hdl.handle.net/10356/81318 http://hdl.handle.net/10220/39220 10.1371/journal.pone.0140743 26485303 en PLoS ONE © 2015 Bendor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited 17 p. application/pdf |
| spellingShingle | Medicine Bendor, Liron Weyrich, Laura S. Linz, Bodo Rolin, Olivier Y. Taylor, Dawn L. Goodfield, Laura L. Smallridge, William E. Kennett, Mary J. Harvill, Eric Thomas Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title | Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title_full | Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title_fullStr | Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title_full_unstemmed | Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title_short | Type Six Secretion System of Bordetella bronchiseptica and Adaptive Immune Components Limit Intracellular Survival During Infection |
| title_sort | type six secretion system of bordetella bronchiseptica and adaptive immune components limit intracellular survival during infection |
| topic | Medicine |
| url | https://hdl.handle.net/10356/81318 http://hdl.handle.net/10220/39220 |
| work_keys_str_mv | AT bendorliron typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT weyrichlauras typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT linzbodo typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT rolinoliviery typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT taylordawnl typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT goodfieldlaural typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT smallridgewilliame typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT kennettmaryj typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection AT harvillericthomas typesixsecretionsystemofbordetellabronchisepticaandadaptiveimmunecomponentslimitintracellularsurvivalduringinfection |