Cleavage efficient 2A peptides for high level monoclonal antibody expression in CHO cells
Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies (mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio and reduces non-expressing cells. Four 2A peptides derived from the foot-and-mouth disease virus (F2A), equine rhinitis...
Main Authors: | Chng, Jake, Wang, Tianhua, Nian, Rui, Lau, Ally, Hoi, Kong Meng, Yang, Yuansheng, Ho, Steven C. L., Gagnon, Peter, Bi, Xuezhi |
---|---|
Other Authors: | School of Chemical and Biomedical Engineering |
Format: | Journal Article |
Language: | English |
Published: |
2016
|
Subjects: | |
Online Access: | https://hdl.handle.net/10356/81450 http://hdl.handle.net/10220/40785 |
Similar Items
-
Analysis of the molecular determinants for furin cleavage of the spike protein S1/S2 site in defined strains of the prototype coronavirus murine hepatitis virus (MHV)
by: Annette Choi, et al.
Published: (2024-02-01) -
Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody
by: Liwei Li, et al.
Published: (2023-10-01) -
Host antiviral factors hijack furin to block SARS-CoV-2, ebola virus, and HIV-1 glycoproteins cleavage
by: Changqing Yu, et al.
Published: (2023-12-01) -
Furin cleavage is required for swine acute diarrhea syndrome coronavirus spike protein-mediated cell – cell fusion
by: Jinman Kim, et al.
Published: (2022-12-01) -
Non-peptide furin inhibitors based on amidinohydrazones of diarylaldehydes
by: V. K. Kibirev, et al.
Published: (2013-02-01)