Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli
We describe an impedimetric microbial sensor for real-time monitoring of the non-lytic M13 bacteriophage infection of Escherichia coli cells using a gold electrode covalently grafted with a monolayer of lipopolysaccharide specific antibody. After infection, damage to the lipopolysaccharide layer on...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/81635 http://hdl.handle.net/10220/40878 |
| _version_ | 1826129153257111552 |
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| author | Cheng, Ming Soon Ho, Jia Shin Lau, Suk Hiang Chow, Vincent T.K. Toh, Chee-Seng |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Cheng, Ming Soon Ho, Jia Shin Lau, Suk Hiang Chow, Vincent T.K. Toh, Chee-Seng |
| author_sort | Cheng, Ming Soon |
| collection | NTU |
| description | We describe an impedimetric microbial sensor for real-time monitoring of the non-lytic M13 bacteriophage infection of Escherichia coli cells using a gold electrode covalently grafted with a monolayer of lipopolysaccharide specific antibody. After infection, damage to the lipopolysaccharide layer on the outer membrane of E. coli causes changes to its surface charge and morphology, resulting in the aggregation of redox probe, Fe(CN)63−/4− at the electrode surface and thereby increases its electron-transfer rate. This consequent decrease of electron-transfer resistance in the presence of bacteriophage can be easily monitored using Faradaic impedance spectroscopy. Non-lytic bacterium–phage interaction which is hardly observable using conventional microscopic methods is detected within 3 h using this impedimetric microbial sensor which demonstrates its excellent performance in terms of analysis time, ease and reduced reliance on labeling steps during in-situ monitoring of the phage infection process. |
| first_indexed | 2024-10-01T07:36:21Z |
| format | Journal Article |
| id | ntu-10356/81635 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:36:21Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/816352020-03-07T12:31:29Z Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli Cheng, Ming Soon Ho, Jia Shin Lau, Suk Hiang Chow, Vincent T.K. Toh, Chee-Seng School of Physical and Mathematical Sciences Biosensor Virus We describe an impedimetric microbial sensor for real-time monitoring of the non-lytic M13 bacteriophage infection of Escherichia coli cells using a gold electrode covalently grafted with a monolayer of lipopolysaccharide specific antibody. After infection, damage to the lipopolysaccharide layer on the outer membrane of E. coli causes changes to its surface charge and morphology, resulting in the aggregation of redox probe, Fe(CN)63−/4− at the electrode surface and thereby increases its electron-transfer rate. This consequent decrease of electron-transfer resistance in the presence of bacteriophage can be easily monitored using Faradaic impedance spectroscopy. Non-lytic bacterium–phage interaction which is hardly observable using conventional microscopic methods is detected within 3 h using this impedimetric microbial sensor which demonstrates its excellent performance in terms of analysis time, ease and reduced reliance on labeling steps during in-situ monitoring of the phage infection process. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-07-01T08:46:59Z 2019-12-06T14:35:20Z 2016-07-01T08:46:59Z 2019-12-06T14:35:20Z 2013 Journal Article Cheng, M. S., Ho, J. S., Lau, S. H., Chow, V. T., & Toh, C.-S. (2013). Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli. Biosensors and Bioelectronics, 47, 340-344. 0956-5663 https://hdl.handle.net/10356/81635 http://hdl.handle.net/10220/40878 10.1016/j.bios.2013.03.050 en Biosensors and Bioelectronics © 2013 Elsevier. |
| spellingShingle | Biosensor Virus Cheng, Ming Soon Ho, Jia Shin Lau, Suk Hiang Chow, Vincent T.K. Toh, Chee-Seng Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title | Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title_full | Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title_fullStr | Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title_full_unstemmed | Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title_short | Impedimetric microbial sensor for real-time monitoring of phage infection of Escherichia coli |
| title_sort | impedimetric microbial sensor for real time monitoring of phage infection of escherichia coli |
| topic | Biosensor Virus |
| url | https://hdl.handle.net/10356/81635 http://hdl.handle.net/10220/40878 |
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