Microfluidic platform for negative enrichment of circulating tumor cells
Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on biomarker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Online Access: | https://hdl.handle.net/10356/81639 http://hdl.handle.net/10220/40875 |
| _version_ | 1824454269300899840 |
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| author | Sajay, Bhuvanendran Nair Gourikutty Chang, Chia-Pin Ahmad, Hamizah Khuntontong, Puttachat Wong, Chee Chung Wang, Zhiping Puiu, Poenar Daniel Soo, Ross Rahman, Abdur Rub Abdur |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Sajay, Bhuvanendran Nair Gourikutty Chang, Chia-Pin Ahmad, Hamizah Khuntontong, Puttachat Wong, Chee Chung Wang, Zhiping Puiu, Poenar Daniel Soo, Ross Rahman, Abdur Rub Abdur |
| author_sort | Sajay, Bhuvanendran Nair Gourikutty |
| collection | NTU |
| description | Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on biomarker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell loss due to extensive sample processing and handling which result in a low recovery efficiency. We present a method that performs negative enrichment in two steps from 2 ml of whole blood in a total assay processing time of 60 min. This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCI-H1975, in the whole blood show an average of >90 % cell recovery over a range of spiked cell numbers. We also successfully recovered circulating tumor cells from 15 cancer patient samples. |
| first_indexed | 2025-02-19T03:19:38Z |
| format | Journal Article |
| id | ntu-10356/81639 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:19:38Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/816392020-03-07T13:57:26Z Microfluidic platform for negative enrichment of circulating tumor cells Sajay, Bhuvanendran Nair Gourikutty Chang, Chia-Pin Ahmad, Hamizah Khuntontong, Puttachat Wong, Chee Chung Wang, Zhiping Puiu, Poenar Daniel Soo, Ross Rahman, Abdur Rub Abdur School of Electrical and Electronic Engineering Circulating tumor cells (CTCs) Cancer cell isolation Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on biomarker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell loss due to extensive sample processing and handling which result in a low recovery efficiency. We present a method that performs negative enrichment in two steps from 2 ml of whole blood in a total assay processing time of 60 min. This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCI-H1975, in the whole blood show an average of >90 % cell recovery over a range of spiked cell numbers. We also successfully recovered circulating tumor cells from 15 cancer patient samples. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-07-01T08:14:38Z 2019-12-06T14:35:23Z 2016-07-01T08:14:38Z 2019-12-06T14:35:23Z 2014 Journal Article Sajay, B. N. G., Chang, C.-P., Ahmad, H., Khuntontong, P., Wong, C. C., Wang, Z., et al. (2014). Microfluidic platform for negative enrichment of circulating tumor cells. Biomedical Microdevices, 16(4), 537-548. 1387-2176 https://hdl.handle.net/10356/81639 http://hdl.handle.net/10220/40875 10.1007/s10544-014-9856-2 en Biomedical Microdevices © 2014 Springer Science+Business Media New York. |
| spellingShingle | Circulating tumor cells (CTCs) Cancer cell isolation Sajay, Bhuvanendran Nair Gourikutty Chang, Chia-Pin Ahmad, Hamizah Khuntontong, Puttachat Wong, Chee Chung Wang, Zhiping Puiu, Poenar Daniel Soo, Ross Rahman, Abdur Rub Abdur Microfluidic platform for negative enrichment of circulating tumor cells |
| title | Microfluidic platform for negative enrichment of circulating tumor cells |
| title_full | Microfluidic platform for negative enrichment of circulating tumor cells |
| title_fullStr | Microfluidic platform for negative enrichment of circulating tumor cells |
| title_full_unstemmed | Microfluidic platform for negative enrichment of circulating tumor cells |
| title_short | Microfluidic platform for negative enrichment of circulating tumor cells |
| title_sort | microfluidic platform for negative enrichment of circulating tumor cells |
| topic | Circulating tumor cells (CTCs) Cancer cell isolation |
| url | https://hdl.handle.net/10356/81639 http://hdl.handle.net/10220/40875 |
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