Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors
Graphene quantum dots (GQDs) are emerging zero-dimensional materials promising a wide spectrum of novel applications including development of optical sensors. Herein, a GQD-based fluorometric sensor is devised to detect acetylcholinesterase (AChE, a critical enzyme in central nervous system and neur...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/81884 http://hdl.handle.net/10220/39723 |
| _version_ | 1811683326607491072 |
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| author | Li, Nan Wang, Xuewan Chen, Jie Sun, Lei Chen, Peng |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Li, Nan Wang, Xuewan Chen, Jie Sun, Lei Chen, Peng |
| author_sort | Li, Nan |
| collection | NTU |
| description | Graphene quantum dots (GQDs) are emerging zero-dimensional materials promising a wide spectrum of novel applications including development of optical sensors. Herein, a GQD-based fluorometric sensor is devised to detect acetylcholinesterase (AChE, a critical enzyme in central nervous system and neuromuscular junctions) with an ultralow detection limit (0.58 pM with S/N of 5.0), using a photoluminescence 'turn-off' mechanism. This simple 'mix-and-detect' platform can also be employed to sense a variety of compounds that can directly or indirectly inhibit the enzymatic activities of AChE, such as nerve gases, pesticides, and therapeutic drugs. As the proof-of-concept demonstrations, we show the sensitive detection of paraoxon (a pesticide), tacrine (a drug to treat Alzheimer's disease), and dopamine (an important neurotransmitter). |
| first_indexed | 2024-10-01T04:10:58Z |
| format | Journal Article |
| id | ntu-10356/81884 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:10:58Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/818842023-12-29T06:47:30Z Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors Li, Nan Wang, Xuewan Chen, Jie Sun, Lei Chen, Peng School of Chemical and Biomedical Engineering 2D Materials Acetylcholinesterase Fluorometric sensor Graphene quantum dots (GQDs) are emerging zero-dimensional materials promising a wide spectrum of novel applications including development of optical sensors. Herein, a GQD-based fluorometric sensor is devised to detect acetylcholinesterase (AChE, a critical enzyme in central nervous system and neuromuscular junctions) with an ultralow detection limit (0.58 pM with S/N of 5.0), using a photoluminescence 'turn-off' mechanism. This simple 'mix-and-detect' platform can also be employed to sense a variety of compounds that can directly or indirectly inhibit the enzymatic activities of AChE, such as nerve gases, pesticides, and therapeutic drugs. As the proof-of-concept demonstrations, we show the sensitive detection of paraoxon (a pesticide), tacrine (a drug to treat Alzheimer's disease), and dopamine (an important neurotransmitter). NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2016-01-20T08:28:46Z 2019-12-06T14:42:19Z 2016-01-20T08:28:46Z 2019-12-06T14:42:19Z 2015 Journal Article Li, N., Wang, X., Chen, J., Sun, L., & Chen, P. (2015). Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors. 2D Materials, 2(3), 034018-. 2053-1583 https://hdl.handle.net/10356/81884 http://hdl.handle.net/10220/39723 10.1088/2053-1583/2/3/034018 en 2D Materials © 2015 IOP Publishing Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by 2D Materials, IOP Publishing Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1088/2053-1583/2/3/034018]. 21 p. application/msword |
| spellingShingle | 2D Materials Acetylcholinesterase Fluorometric sensor Li, Nan Wang, Xuewan Chen, Jie Sun, Lei Chen, Peng Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title | Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title_full | Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title_fullStr | Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title_full_unstemmed | Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title_short | Graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| title_sort | graphene quantum dots for ultrasensitive detection of acetylcholinesterase and its inhibitors |
| topic | 2D Materials Acetylcholinesterase Fluorometric sensor |
| url | https://hdl.handle.net/10356/81884 http://hdl.handle.net/10220/39723 |
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