Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography
The mucosal microanatomy of the large intestine is characterized by the presence of crypts of Lieberkühn, which is associated predominantly with goblet cells. Such cellular-level intestinal microstructures undergo morphological changes during the progression of bowel diseases, such as colon cancer...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/81959 http://hdl.handle.net/10220/42283 |
| _version_ | 1826110885417975808 |
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| author | Yu, Xiaojun Luo, Yuemei Liu, Xinyu Chen, Si Wang, Xianghong Chen, Shi Liu, Linbo |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yu, Xiaojun Luo, Yuemei Liu, Xinyu Chen, Si Wang, Xianghong Chen, Shi Liu, Linbo |
| author_sort | Yu, Xiaojun |
| collection | NTU |
| description | The mucosal microanatomy of the large intestine is characterized by the presence of crypts of Lieberkühn, which is associated predominantly with goblet cells. Such cellular-level intestinal microstructures undergo morphological changes during the progression of bowel diseases, such as colon cancer or ulcerative colitis. As an indicator of gastric cancers, intestinal metaplasia in the large intestine is characterized by the appearance of goblet cells in gastric epithelium, and therefore, visualization of intestinal microstructure changes in cross-sectional view, particularly in vivo, in a high-speed fashion would assist early disease diagnosis and its treatment. In this paper, we investigated the capability of micro-optical coherence tomography (μOCT) for high-speed cellular-level crypt and goblet cell structures imaging ex vivo and in vivo . The adopted μOCT system achieved a resolution of 2.0 μm in both the lateral and axial directions in air. Ex vivo and video-rate in vivo images acquired in 3-D at respective imaging rates of 20 and 60 frames/s are presented and compared with the histology images. Imaging results show that the detailed microstructures, such as the crypt lumen and the goblet cells, could be clearly identified and are also comparable with those in histology images. Such comparisons also indicate that high-resolution μOCT could be a powerful tool to perform “optical biopsy” in colorectal tissue. This is the first work, to the best of our knowledge, on cellular-level structure imaging in intestinal mucosa using spectral-domain OCT. |
| first_indexed | 2024-10-01T02:41:29Z |
| format | Journal Article |
| id | ntu-10356/81959 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:41:29Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/819592020-03-07T11:35:25Z Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography Yu, Xiaojun Luo, Yuemei Liu, Xinyu Chen, Si Wang, Xianghong Chen, Shi Liu, Linbo School of Chemical and Biomedical Engineering School of Electrical and Electronic Engineering Optical coherence tomography Biophotonics instrumentation The mucosal microanatomy of the large intestine is characterized by the presence of crypts of Lieberkühn, which is associated predominantly with goblet cells. Such cellular-level intestinal microstructures undergo morphological changes during the progression of bowel diseases, such as colon cancer or ulcerative colitis. As an indicator of gastric cancers, intestinal metaplasia in the large intestine is characterized by the appearance of goblet cells in gastric epithelium, and therefore, visualization of intestinal microstructure changes in cross-sectional view, particularly in vivo, in a high-speed fashion would assist early disease diagnosis and its treatment. In this paper, we investigated the capability of micro-optical coherence tomography (μOCT) for high-speed cellular-level crypt and goblet cell structures imaging ex vivo and in vivo . The adopted μOCT system achieved a resolution of 2.0 μm in both the lateral and axial directions in air. Ex vivo and video-rate in vivo images acquired in 3-D at respective imaging rates of 20 and 60 frames/s are presented and compared with the histology images. Imaging results show that the detailed microstructures, such as the crypt lumen and the goblet cells, could be clearly identified and are also comparable with those in histology images. Such comparisons also indicate that high-resolution μOCT could be a powerful tool to perform “optical biopsy” in colorectal tissue. This is the first work, to the best of our knowledge, on cellular-level structure imaging in intestinal mucosa using spectral-domain OCT. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) NMRC (Natl Medical Research Council, S’pore) Published version 2017-04-19T03:33:58Z 2019-12-06T14:43:49Z 2017-04-19T03:33:58Z 2019-12-06T14:43:49Z 2016 2016 Journal Article Yu, X., Luo, Y., Liu, X., Chen, S., Wang, X., Chen, S., et al. (2016). Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography. IEEE Photonics Journal, 8(4), 3900308-. 1943-0655 https://hdl.handle.net/10356/81959 http://hdl.handle.net/10220/42283 10.1109/JPHOT.2016.2589659 198111 en IEEE Photonics Journal © 2016 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. The published version is available at: [https://doi.org/10.1109/JPHOT.2016.2589659]. 9 p. application/pdf |
| spellingShingle | Optical coherence tomography Biophotonics instrumentation Yu, Xiaojun Luo, Yuemei Liu, Xinyu Chen, Si Wang, Xianghong Chen, Shi Liu, Linbo Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title | Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title_full | Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title_fullStr | Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title_full_unstemmed | Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title_short | Toward High-Speed Imaging of Cellular Structures in Rat Colon Using Micro-optical Coherence Tomography |
| title_sort | toward high speed imaging of cellular structures in rat colon using micro optical coherence tomography |
| topic | Optical coherence tomography Biophotonics instrumentation |
| url | https://hdl.handle.net/10356/81959 http://hdl.handle.net/10220/42283 |
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