Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy
Gold nanorods have the potential to localize the treatment procedure by hyperthermia and influence the fluorescence. The longitudinal plasmon peak contributes to the photothermal effect by converting light to heat. When these nanorods are PEGylated, it not only makes it biocompatible but also acts a...
| Main Authors: | , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/82003 http://hdl.handle.net/10220/39751 |
| _version_ | 1826113268136017920 |
|---|---|
| author | Kannadorai, Ravi Kumar Chiew, Geraldine Giap Ying Luo, Kathy Qian Liu, Quan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Kannadorai, Ravi Kumar Chiew, Geraldine Giap Ying Luo, Kathy Qian Liu, Quan |
| author_sort | Kannadorai, Ravi Kumar |
| collection | NTU |
| description | Gold nanorods have the potential to localize the treatment procedure by hyperthermia and influence the fluorescence. The longitudinal plasmon peak contributes to the photothermal effect by converting light to heat. When these nanorods are PEGylated, it not only makes it biocompatible but also acts as a spacer layer during fluorescence enhancement. When the PEGylated nanorods are internalized inside the cells through endocytosis, the transverse plasmonic peak combined with the enhanced absorption and scattering properties of the nanorods can enhance the autofluorescence emission intensity from the cell. The autofluorescence from the mitochondria inside cells which reflects the respiratory status of the cell was enhanced two times by the presence of nanorods within the cell. At four minutes, the nanorods incubated cells reached the hyperthermic temperature when illuminated continuously with near infrared laser. The cell viability test and autofluorescence intensity curve showed a similar trend indicating the progress of cell death over time. This is the first report to the best of our knowledge to suggest the potential of exploiting the dual capabilities of gold nanorods as photothermal agents and autofluorescence enhancer to track cell death. |
| first_indexed | 2024-10-01T03:20:24Z |
| format | Journal Article |
| id | ntu-10356/82003 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:20:24Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/820032023-12-29T06:46:28Z Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy Kannadorai, Ravi Kumar Chiew, Geraldine Giap Ying Luo, Kathy Qian Liu, Quan School of Chemical and Biomedical Engineering Photothermal therapy Gold nanorods Necrosis Autofluorescence Renal cell carcinoma Hyperthermia Gold nanorods have the potential to localize the treatment procedure by hyperthermia and influence the fluorescence. The longitudinal plasmon peak contributes to the photothermal effect by converting light to heat. When these nanorods are PEGylated, it not only makes it biocompatible but also acts as a spacer layer during fluorescence enhancement. When the PEGylated nanorods are internalized inside the cells through endocytosis, the transverse plasmonic peak combined with the enhanced absorption and scattering properties of the nanorods can enhance the autofluorescence emission intensity from the cell. The autofluorescence from the mitochondria inside cells which reflects the respiratory status of the cell was enhanced two times by the presence of nanorods within the cell. At four minutes, the nanorods incubated cells reached the hyperthermic temperature when illuminated continuously with near infrared laser. The cell viability test and autofluorescence intensity curve showed a similar trend indicating the progress of cell death over time. This is the first report to the best of our knowledge to suggest the potential of exploiting the dual capabilities of gold nanorods as photothermal agents and autofluorescence enhancer to track cell death. ASTAR (Agency for Sci., Tech. and Research, S’pore) Accepted version 2016-01-22T03:32:36Z 2019-12-06T14:44:32Z 2016-01-22T03:32:36Z 2019-12-06T14:44:32Z 2014 Journal Article Kannadorai, R. K., Chiew, G. G. Y., Luo, K. Q., & Liu, Q. (2014). Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy. Cancer Letters, 357(1), 152-159. 0304-3835 https://hdl.handle.net/10356/82003 http://hdl.handle.net/10220/39751 10.1016/j.canlet.2014.11.022 en Cancer Letters © 2014 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Cancer Letters, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.canlet.2014.11.022]. 24 p. application/pdf |
| spellingShingle | Photothermal therapy Gold nanorods Necrosis Autofluorescence Renal cell carcinoma Hyperthermia Kannadorai, Ravi Kumar Chiew, Geraldine Giap Ying Luo, Kathy Qian Liu, Quan Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title | Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title_full | Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title_fullStr | Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title_full_unstemmed | Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title_short | Dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| title_sort | dual functions of gold nanorods as photothermal agent and autofluorescence enhancer to track cell death during plasmonic photothermal therapy |
| topic | Photothermal therapy Gold nanorods Necrosis Autofluorescence Renal cell carcinoma Hyperthermia |
| url | https://hdl.handle.net/10356/82003 http://hdl.handle.net/10220/39751 |
| work_keys_str_mv | AT kannadorairavikumar dualfunctionsofgoldnanorodsasphotothermalagentandautofluorescenceenhancertotrackcelldeathduringplasmonicphotothermaltherapy AT chiewgeraldinegiapying dualfunctionsofgoldnanorodsasphotothermalagentandautofluorescenceenhancertotrackcelldeathduringplasmonicphotothermaltherapy AT luokathyqian dualfunctionsofgoldnanorodsasphotothermalagentandautofluorescenceenhancertotrackcelldeathduringplasmonicphotothermaltherapy AT liuquan dualfunctionsofgoldnanorodsasphotothermalagentandautofluorescenceenhancertotrackcelldeathduringplasmonicphotothermaltherapy |