Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots
We designed and fabricated two new nanostructured biosensing chips, with which the sensitive detection of prostate specific antigen (PSA) as low as 100 pg ml−1 can be achieved, by measuring the plasmon enhanced fluorescence through a conventional dark field microscope. The gold nanostructure arrays,...
| Main Authors: | , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Online Access: | https://hdl.handle.net/10356/82075 http://hdl.handle.net/10220/41105 |
| _version_ | 1826127796683931648 |
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| author | Song, Hong Yan Wong, Ten It Sadovoy, Anton Wu, Lin Bai, Ping Deng, Jie Guo, Shifeng Wang, Yi Knoll, Wolfgang Zhou, Xiaodong |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Song, Hong Yan Wong, Ten It Sadovoy, Anton Wu, Lin Bai, Ping Deng, Jie Guo, Shifeng Wang, Yi Knoll, Wolfgang Zhou, Xiaodong |
| author_sort | Song, Hong Yan |
| collection | NTU |
| description | We designed and fabricated two new nanostructured biosensing chips, with which the sensitive detection of prostate specific antigen (PSA) as low as 100 pg ml−1 can be achieved, by measuring the plasmon enhanced fluorescence through a conventional dark field microscope. The gold nanostructure arrays, one with gold nanopillars of 140 nm, the other with gold nanoholes of 140 nm, were fabricated via nanoimprinting onto glass substrate, as localized surface plasmon resonance (LSPR) generators to enhance the fluorescent emission of fluorophore, e.g. quantum dot (QD). A sandwich bioassay of capture anti-PSA antibody (cAb)/PSA/detection anti-PSA (dAb) labeled by QD-655 was established on the nanostructures, and the perfect LSPR excitation distance (10–15 nm) between the nanostructure and QD-655 was simulated and controlled by a cleft cAb fragment and streptavidin modified QD. QD was chosen in this study due to its photo stability, broad Stokes shift, and long lifetime. As far as we know, this is the first time that QD is applied for PSA detection on the uniform nanostructured sensing chips based on the LSPR enhanced fluorescence. Due to the miniaturized nanoarray sensing chip (1.8 mm × 1.8 mm), the convenience and specificity for the detection of PSA via the sandwich assay, and the high optical detection sensitivity, the platform has great potential for the development of a portable point-of-care (POC) system for outpatient diagnosis and treatment monitoring. |
| first_indexed | 2024-10-01T07:14:22Z |
| format | Journal Article |
| id | ntu-10356/82075 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:14:22Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/820752020-06-01T10:13:44Z Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots Song, Hong Yan Wong, Ten It Sadovoy, Anton Wu, Lin Bai, Ping Deng, Jie Guo, Shifeng Wang, Yi Knoll, Wolfgang Zhou, Xiaodong School of Materials Science & Engineering We designed and fabricated two new nanostructured biosensing chips, with which the sensitive detection of prostate specific antigen (PSA) as low as 100 pg ml−1 can be achieved, by measuring the plasmon enhanced fluorescence through a conventional dark field microscope. The gold nanostructure arrays, one with gold nanopillars of 140 nm, the other with gold nanoholes of 140 nm, were fabricated via nanoimprinting onto glass substrate, as localized surface plasmon resonance (LSPR) generators to enhance the fluorescent emission of fluorophore, e.g. quantum dot (QD). A sandwich bioassay of capture anti-PSA antibody (cAb)/PSA/detection anti-PSA (dAb) labeled by QD-655 was established on the nanostructures, and the perfect LSPR excitation distance (10–15 nm) between the nanostructure and QD-655 was simulated and controlled by a cleft cAb fragment and streptavidin modified QD. QD was chosen in this study due to its photo stability, broad Stokes shift, and long lifetime. As far as we know, this is the first time that QD is applied for PSA detection on the uniform nanostructured sensing chips based on the LSPR enhanced fluorescence. Due to the miniaturized nanoarray sensing chip (1.8 mm × 1.8 mm), the convenience and specificity for the detection of PSA via the sandwich assay, and the high optical detection sensitivity, the platform has great potential for the development of a portable point-of-care (POC) system for outpatient diagnosis and treatment monitoring. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-08-05T07:26:45Z 2019-12-06T14:46:00Z 2016-08-05T07:26:45Z 2019-12-06T14:46:00Z 2014 Journal Article Song, H. Y., Wong, T. I., Sadovoy, A., Wu, L., Bai, P., Deng, J., et al. (2015). Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots. Lab Chip, 15(1), 253-263. https://hdl.handle.net/10356/82075 http://hdl.handle.net/10220/41105 10.1039/C4LC00978A en Lab Chip © 2015 The Royal Society of Chemistry. |
| spellingShingle | Song, Hong Yan Wong, Ten It Sadovoy, Anton Wu, Lin Bai, Ping Deng, Jie Guo, Shifeng Wang, Yi Knoll, Wolfgang Zhou, Xiaodong Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title | Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title_full | Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title_fullStr | Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title_full_unstemmed | Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title_short | Imprinted gold 2D nanoarray for highly sensitive and convenient PSA detection via plasmon excited quantum dots |
| title_sort | imprinted gold 2d nanoarray for highly sensitive and convenient psa detection via plasmon excited quantum dots |
| url | https://hdl.handle.net/10356/82075 http://hdl.handle.net/10220/41105 |
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