Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system
This study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanom...
| Autores principales: | , , , , |
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| Otros Autores: | |
| Formato: | Journal Article |
| Lenguaje: | English |
| Publicado: |
2016
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| Acceso en línea: | https://hdl.handle.net/10356/82795 http://hdl.handle.net/10220/40285 |
| _version_ | 1826113857334018048 |
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| author | Xiao, Keke Guo, Chenghong Zhou, Yan Maspolim, Yogananda Ng, Wun-Jern |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Xiao, Keke Guo, Chenghong Zhou, Yan Maspolim, Yogananda Ng, Wun-Jern |
| author_sort | Xiao, Keke |
| collection | NTU |
| description | This study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanomicrobiales was the predominant archaeal population in the second stage (>99% of the total archaeal community). Second stage biomass degraded HAc up to 4200 mg HAc L-1 without observable lag phase. However, at HAc-shock loading of 7400 mg HAc L-1, it showed a one day lag phase associated with decreased biomass activity. After stepwise HAc-acclimation over 27 d, the biomass degraded HAc of up to 8200 mg HAc L-1 without observable lag phase. The dominance of Methanomicrobiales had remained unchanged in proportion - while the total archaeal population increased during acclimation. This study showed stepwise acclimation could be an approach to accommodate HAc accumulation and hence higher concentrations resulting from an enhanced first stage. Keywords: Acclimation; Acetic acid inhibition; Biomass activity; Hydrogenotrophic methanogens; Pyrosequencing; Two-stage anaerobic system |
| first_indexed | 2024-10-01T03:30:05Z |
| format | Journal Article |
| id | ntu-10356/82795 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:30:05Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/827952020-09-26T21:58:37Z Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system Xiao, Keke Guo, Chenghong Zhou, Yan Maspolim, Yogananda Ng, Wun-Jern School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute Two-stage anaerobic system Acetic acid inhibition Acclimation Hydrogenotrophic methanogens Pyrosequencing Biomass activity This study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanomicrobiales was the predominant archaeal population in the second stage (>99% of the total archaeal community). Second stage biomass degraded HAc up to 4200 mg HAc L-1 without observable lag phase. However, at HAc-shock loading of 7400 mg HAc L-1, it showed a one day lag phase associated with decreased biomass activity. After stepwise HAc-acclimation over 27 d, the biomass degraded HAc of up to 8200 mg HAc L-1 without observable lag phase. The dominance of Methanomicrobiales had remained unchanged in proportion - while the total archaeal population increased during acclimation. This study showed stepwise acclimation could be an approach to accommodate HAc accumulation and hence higher concentrations resulting from an enhanced first stage. Keywords: Acclimation; Acetic acid inhibition; Biomass activity; Hydrogenotrophic methanogens; Pyrosequencing; Two-stage anaerobic system NRF (Natl Research Foundation, S’pore) Accepted version 2016-03-15T07:23:05Z 2019-12-06T15:05:44Z 2016-03-15T07:23:05Z 2019-12-06T15:05:44Z 2015 Journal Article Xiao, K., Guo, C., Zhou, Y., Maspolim, Y., & Ng, W.-J. (2016). Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system. Chemosphere, 144, 1498-1504. 0045-6535 https://hdl.handle.net/10356/82795 http://hdl.handle.net/10220/40285 10.1016/j.chemosphere.2015.10.035 en Chemosphere © 2015 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemosphere, Elsevier Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.chemosphere.2015.10.035]. 20 p. application/pdf |
| spellingShingle | Two-stage anaerobic system Acetic acid inhibition Acclimation Hydrogenotrophic methanogens Pyrosequencing Biomass activity Xiao, Keke Guo, Chenghong Zhou, Yan Maspolim, Yogananda Ng, Wun-Jern Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title | Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title_full | Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title_fullStr | Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title_full_unstemmed | Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title_short | Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system |
| title_sort | acetic acid effects on methanogens in the second stage of a two stage anaerobic system |
| topic | Two-stage anaerobic system Acetic acid inhibition Acclimation Hydrogenotrophic methanogens Pyrosequencing Biomass activity |
| url | https://hdl.handle.net/10356/82795 http://hdl.handle.net/10220/40285 |
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