An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures
We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the natur...
| Main Authors: | , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/82904 http://hdl.handle.net/10220/40359 |
| _version_ | 1826122305980334080 |
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| author | Zhang, Hao Ba, Sai Co Co, Sarajane Lee, Jasmine Yiqin Guo, Juanjuan Ye, Ruijuan Liang, Zhaoxun Huang, Dejian Li, Tianhu |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Zhang, Hao Ba, Sai Co Co, Sarajane Lee, Jasmine Yiqin Guo, Juanjuan Ye, Ruijuan Liang, Zhaoxun Huang, Dejian Li, Tianhu |
| author_sort | Zhang, Hao |
| collection | NTU |
| description | We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence. |
| first_indexed | 2024-10-01T05:45:59Z |
| format | Journal Article |
| id | ntu-10356/82904 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:45:59Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/829042020-03-07T12:18:08Z An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures Zhang, Hao Ba, Sai Co Co, Sarajane Lee, Jasmine Yiqin Guo, Juanjuan Ye, Ruijuan Liang, Zhaoxun Huang, Dejian Li, Tianhu School of Biological Sciences School of Physical and Mathematical Sciences Oligonucleotides DNA ligases We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the nature of the loop sequence. MOE (Min. of Education, S’pore) 2016-03-31T09:21:07Z 2019-12-06T15:07:53Z 2016-03-31T09:21:07Z 2019-12-06T15:07:53Z 2015 Journal Article Zhang, H., Ba, S., Co Co, S., Lee, J. Y., Guo, J., Ye, R., et al. (2015). An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures. Bulletin of the Chemical Society of Japan, 88(9), 1314-1316. 0009-2673 https://hdl.handle.net/10356/82904 http://hdl.handle.net/10220/40359 10.1246/bcsj.20150120 en Bulletin of the Chemical Society of Japan © 2015 The Chemical Society of Japan. |
| spellingShingle | Oligonucleotides DNA ligases Zhang, Hao Ba, Sai Co Co, Sarajane Lee, Jasmine Yiqin Guo, Juanjuan Ye, Ruijuan Liang, Zhaoxun Huang, Dejian Li, Tianhu An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title | An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title_full | An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title_fullStr | An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title_full_unstemmed | An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title_short | An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures |
| title_sort | alternative method for evaluating stabilities of dna hairpin structures |
| topic | Oligonucleotides DNA ligases |
| url | https://hdl.handle.net/10356/82904 http://hdl.handle.net/10220/40359 |
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