Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector
Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scatterin...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/83020 http://hdl.handle.net/10220/44134 |
| _version_ | 1811678863711797248 |
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| author | Tian, Yao Su, Joshua Weiming Ju, Jian Liu, Quan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Tian, Yao Su, Joshua Weiming Ju, Jian Liu, Quan |
| author_sort | Tian, Yao |
| collection | NTU |
| description | Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scattering of photons. To achieve a high signal level, usually a high numerical aperture objective is employed. One drawback with these objectives is that their working distance is very short. However, in many cases of clinical diagnostics, a long working distance is preferable. We propose a practical solution to this problem by enhancing the Raman signal using a parabolic reflector. The high signal level is achieved through the large light collection solid angle of the parabolic reflector while the long working distance is ensured by the novel design of our microscope. The enhancement capability of the microscope was demonstrated on four types of samples. Among these samples, we find that this microscope design is most suitable for turbid samples. |
| first_indexed | 2024-10-01T03:00:01Z |
| format | Journal Article |
| id | ntu-10356/83020 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:00:01Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/830202023-12-29T06:45:59Z Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector Tian, Yao Su, Joshua Weiming Ju, Jian Liu, Quan School of Chemical and Biomedical Engineering Raman signal enhancement Parabolic reflector Raman spectroscopy is well suited for readily revealing information about bio-samples. As such, this technique has been applied to a wide range of areas, particularly in bio-medical diagnostics. Raman scattering in bio-samples typically has a low signal level due to the nature of inelastic scattering of photons. To achieve a high signal level, usually a high numerical aperture objective is employed. One drawback with these objectives is that their working distance is very short. However, in many cases of clinical diagnostics, a long working distance is preferable. We propose a practical solution to this problem by enhancing the Raman signal using a parabolic reflector. The high signal level is achieved through the large light collection solid angle of the parabolic reflector while the long working distance is ensured by the novel design of our microscope. The enhancement capability of the microscope was demonstrated on four types of samples. Among these samples, we find that this microscope design is most suitable for turbid samples. MOE (Min. of Education, S’pore) Accepted version 2017-12-13T02:05:37Z 2019-12-06T15:10:19Z 2017-12-13T02:05:37Z 2019-12-06T15:10:19Z 2017 2017 Journal Article Tian, Y., Su, J. W., Ju, J., & Liu, Q. (2017). Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector. Biomedical Optics Express, 8(11), 5243-5252. https://hdl.handle.net/10356/83020 http://hdl.handle.net/10220/44134 10.1364/BOE.8.005243 199663 en Biomedical Optics Express © 2017 Optical Society of America. This is the author created version of a work that has been peer reviewed and accepted for publication by Biomedical Optics Express, Optical Society of America. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1364/BOE.8.005243]. 11 p. application/pdf |
| spellingShingle | Raman signal enhancement Parabolic reflector Tian, Yao Su, Joshua Weiming Ju, Jian Liu, Quan Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_full | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_fullStr | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_full_unstemmed | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_short | Efficiency enhancement of Raman spectroscopy at long working distance by parabolic reflector |
| title_sort | efficiency enhancement of raman spectroscopy at long working distance by parabolic reflector |
| topic | Raman signal enhancement Parabolic reflector |
| url | https://hdl.handle.net/10356/83020 http://hdl.handle.net/10220/44134 |
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