Functional impact of splice isoform diversity in individual cells
Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such...
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/83044 http://hdl.handle.net/10220/42378 |
| _version_ | 1811682695959281664 |
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| author | Yap, Karen Makeyev, Eugene V. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Yap, Karen Makeyev, Eugene V. |
| author_sort | Yap, Karen |
| collection | NTU |
| description | Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such co-expression is biologically meaningful or, rather, represents insufficiently stringent splicing regulation. Here we argue that isoform co-expression may produce functional outcomes that are difficult and sometimes impossible to achieve using other regulation strategies. Far from being a ‘splicing noise’, co-expression is often established through co-ordinated activity of specific cis-elements and trans-acting factors. Further work in this area may uncover new biological functions of alternative splicing (AS) and generate important insights into mechanisms allowing different cell types to attain their unique molecular identities. |
| first_indexed | 2024-10-01T04:00:56Z |
| format | Journal Article |
| id | ntu-10356/83044 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:00:56Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/830442023-02-28T17:00:09Z Functional impact of splice isoform diversity in individual cells Yap, Karen Makeyev, Eugene V. School of Biological Sciences Alternative splicing Isoform co-expression Alternative pre-mRNA splicing provides an effective means for expanding coding capacity of eukaryotic genomes. Recent studies suggest that co-expression of different splice isoforms may increase diversity of RNAs and proteins at a single-cell level. A pertinent question in the field is whether such co-expression is biologically meaningful or, rather, represents insufficiently stringent splicing regulation. Here we argue that isoform co-expression may produce functional outcomes that are difficult and sometimes impossible to achieve using other regulation strategies. Far from being a ‘splicing noise’, co-expression is often established through co-ordinated activity of specific cis-elements and trans-acting factors. Further work in this area may uncover new biological functions of alternative splicing (AS) and generate important insights into mechanisms allowing different cell types to attain their unique molecular identities. NMRC (Natl Medical Research Council, S’pore) Published version 2017-05-11T07:20:53Z 2019-12-06T15:10:47Z 2017-05-11T07:20:53Z 2019-12-06T15:10:47Z 2016 Journal Article Yap, K., & Makeyev, E. V. (2016). Functional impact of splice isoform diversity in individual cells. Biochemical Society Transactions, 44(4), 1079-1085. 0300-5127 https://hdl.handle.net/10356/83044 http://hdl.handle.net/10220/42378 10.1042/BST20160103 27528755 en Biochemical Society Transactions © 2016 The Author(s). This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY). 7 p. application/pdf |
| spellingShingle | Alternative splicing Isoform co-expression Yap, Karen Makeyev, Eugene V. Functional impact of splice isoform diversity in individual cells |
| title | Functional impact of splice isoform diversity in individual cells |
| title_full | Functional impact of splice isoform diversity in individual cells |
| title_fullStr | Functional impact of splice isoform diversity in individual cells |
| title_full_unstemmed | Functional impact of splice isoform diversity in individual cells |
| title_short | Functional impact of splice isoform diversity in individual cells |
| title_sort | functional impact of splice isoform diversity in individual cells |
| topic | Alternative splicing Isoform co-expression |
| url | https://hdl.handle.net/10356/83044 http://hdl.handle.net/10220/42378 |
| work_keys_str_mv | AT yapkaren functionalimpactofspliceisoformdiversityinindividualcells AT makeyeveugenev functionalimpactofspliceisoformdiversityinindividualcells |