Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles
Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/83047 http://hdl.handle.net/10220/42374 |
| _version_ | 1826124638784061440 |
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| author | Li, Yan Wong, Ying Lei Lee, Michelle Yueqi Li, Qingxin Wang, Qing-Yin Lescar, Julien Shi, Pei-Yong Kang, CongBao |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Li, Yan Wong, Ying Lei Lee, Michelle Yueqi Li, Qingxin Wang, Qing-Yin Lescar, Julien Shi, Pei-Yong Kang, CongBao |
| author_sort | Li, Yan |
| collection | NTU |
| description | Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections. |
| first_indexed | 2024-10-01T06:23:39Z |
| format | Journal Article |
| id | ntu-10356/83047 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:23:39Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/830472023-02-28T17:00:13Z Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles Li, Yan Wong, Ying Lei Lee, Michelle Yueqi Li, Qingxin Wang, Qing-Yin Lescar, Julien Shi, Pei-Yong Kang, CongBao School of Biological Sciences Dengue-Virus Membranproteine Dengue virus nonstructural protein 4B (NS4B) is a membrane protein consisting of 248 residues with a crucial role in virus replication and interference with the host innate immunity. The dengue virus serotype 3 NS4B was reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. Backbone resonance assignment of NS4B was obtained using conventional solution NMR experiments. Further studies suggested that NS4B contained eleven helices and six of them form five potential transmembrane regions. This study provides atomic level information for an important drug target to control flavivirus infections. ASTAR (Agency for Sci., Tech. and Research, S’pore) NMRC (Natl Medical Research Council, S’pore) Accepted version 2017-05-11T06:21:31Z 2019-12-06T15:10:50Z 2017-05-11T06:21:31Z 2019-12-06T15:10:50Z 2016 Journal Article Li, Y., Wong, Y. L., Lee, M. Y., Li, Q., Wang, Q.-Y., Lescar, J., et al. (2016). Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles. Angewandte Chemie International Edition, 55(39), 12068-12072. 1433-7851 https://hdl.handle.net/10356/83047 http://hdl.handle.net/10220/42374 10.1002/anie.201606609 en Angewandte Chemie International Edition © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Angewandte Chemie International Edition, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/anie.201606609]. 9 p. application/pdf |
| spellingShingle | Dengue-Virus Membranproteine Li, Yan Wong, Ying Lei Lee, Michelle Yueqi Li, Qingxin Wang, Qing-Yin Lescar, Julien Shi, Pei-Yong Kang, CongBao Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title | Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title_full | Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title_fullStr | Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title_full_unstemmed | Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title_short | Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles |
| title_sort | secondary structure and membrane topology of the full length dengue virus ns4b in micelles |
| topic | Dengue-Virus Membranproteine |
| url | https://hdl.handle.net/10356/83047 http://hdl.handle.net/10220/42374 |
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