Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis
Reactive oxygen species (ROS) are key signalling intermediates in plant metabolism, defence, and stress adaptation. In plants, both the chloroplast and mitochondria are centres of metabolic control and ROS production, which coordinate stress responses in other cell compartments. The herbicide and ex...
| Główni autorzy: | , , , , , , , , , , |
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| Kolejni autorzy: | |
| Format: | Journal Article |
| Język: | English |
| Wydane: |
2019
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| Hasła przedmiotowe: | |
| Dostęp online: | https://hdl.handle.net/10356/83076 http://hdl.handle.net/10220/49743 |
| _version_ | 1826120605945036800 |
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| author | Cui, Fuqiang Brosché, Mikael Shapiguzov, Alexey He, Xin-Qiang Vainonen, Julia P. Leppälä, Johanna Trotta, Andrea Kangasjärvi, Saijaliisa Salojärvi, Jarkko Kangasjärvi, Jaakko Overmyer, Kirk |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Cui, Fuqiang Brosché, Mikael Shapiguzov, Alexey He, Xin-Qiang Vainonen, Julia P. Leppälä, Johanna Trotta, Andrea Kangasjärvi, Saijaliisa Salojärvi, Jarkko Kangasjärvi, Jaakko Overmyer, Kirk |
| author_sort | Cui, Fuqiang |
| collection | NTU |
| description | Reactive oxygen species (ROS) are key signalling intermediates in plant metabolism, defence, and stress adaptation. In plants, both the chloroplast and mitochondria are centres of metabolic control and ROS production, which coordinate stress responses in other cell compartments. The herbicide and experimental tool, methyl viologen (MV) induces ROS generation in the chloroplast under illumination, but is also toxic in non-photosynthetic organisms. We used MV to probe plant ROS signalling in compartments other than the chloroplast. Taking a genetic approach in the model plant Arabidopsis (Arabidopsis thaliana), we used natural variation, QTL mapping, and mutant studies with MV in the light, but also under dark conditions, when the chloroplast electron transport is inactive. These studies revealed a light-independent MV-induced ROS-signalling pathway, suggesting mitochondrial involvement. Mitochondrial Mn SUPEROXIDE DISMUTASE was required for ROS-tolerance and the effect of MV was enhanced by exogenous sugar, providing further evidence for the role of mitochondria. Mutant and hormone feeding assays revealed roles for stress hormones in organellar ROS-responses. The radical-induced cell death1 mutant, which is tolerant to MV-induced ROS and exhibits altered mitochondrial signalling, was used to probe interactions between organelles. Our studies suggest that mitochondria are involved in the response to ROS induced by MV in plants. |
| first_indexed | 2024-10-01T05:19:11Z |
| format | Journal Article |
| id | ntu-10356/83076 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:19:11Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/830762023-02-28T17:00:15Z Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis Cui, Fuqiang Brosché, Mikael Shapiguzov, Alexey He, Xin-Qiang Vainonen, Julia P. Leppälä, Johanna Trotta, Andrea Kangasjärvi, Saijaliisa Salojärvi, Jarkko Kangasjärvi, Jaakko Overmyer, Kirk School of Biological Sciences Chloroplast Science::Biological sciences Arabidopsis Thaliana Reactive oxygen species (ROS) are key signalling intermediates in plant metabolism, defence, and stress adaptation. In plants, both the chloroplast and mitochondria are centres of metabolic control and ROS production, which coordinate stress responses in other cell compartments. The herbicide and experimental tool, methyl viologen (MV) induces ROS generation in the chloroplast under illumination, but is also toxic in non-photosynthetic organisms. We used MV to probe plant ROS signalling in compartments other than the chloroplast. Taking a genetic approach in the model plant Arabidopsis (Arabidopsis thaliana), we used natural variation, QTL mapping, and mutant studies with MV in the light, but also under dark conditions, when the chloroplast electron transport is inactive. These studies revealed a light-independent MV-induced ROS-signalling pathway, suggesting mitochondrial involvement. Mitochondrial Mn SUPEROXIDE DISMUTASE was required for ROS-tolerance and the effect of MV was enhanced by exogenous sugar, providing further evidence for the role of mitochondria. Mutant and hormone feeding assays revealed roles for stress hormones in organellar ROS-responses. The radical-induced cell death1 mutant, which is tolerant to MV-induced ROS and exhibits altered mitochondrial signalling, was used to probe interactions between organelles. Our studies suggest that mitochondria are involved in the response to ROS induced by MV in plants. Published version 2019-08-22T04:38:45Z 2019-12-06T15:11:20Z 2019-08-22T04:38:45Z 2019-12-06T15:11:20Z 2019 Journal Article Cui, F., Brosché, M., Shapiguzov, A., He, X.-Q., Vainonen, J. P., Leppälä, J., . . . Overmyer, K. (2019). Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis. Free Radical Biology and Medicine, 134, 555-566. doi:10.1016/j.freeradbiomed.2019.02.006 0891-5849 https://hdl.handle.net/10356/83076 http://hdl.handle.net/10220/49743 10.1016/j.freeradbiomed.2019.02.006 en Free Radical Biology and Medicine © 2019 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). 12 p. application/pdf |
| spellingShingle | Chloroplast Science::Biological sciences Arabidopsis Thaliana Cui, Fuqiang Brosché, Mikael Shapiguzov, Alexey He, Xin-Qiang Vainonen, Julia P. Leppälä, Johanna Trotta, Andrea Kangasjärvi, Saijaliisa Salojärvi, Jarkko Kangasjärvi, Jaakko Overmyer, Kirk Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title | Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title_full | Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title_fullStr | Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title_full_unstemmed | Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title_short | Interaction of methyl viologen-induced chloroplast and mitochondrial signalling in Arabidopsis |
| title_sort | interaction of methyl viologen induced chloroplast and mitochondrial signalling in arabidopsis |
| topic | Chloroplast Science::Biological sciences Arabidopsis Thaliana |
| url | https://hdl.handle.net/10356/83076 http://hdl.handle.net/10220/49743 |
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