Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing
Antisense oligodeoxynucleotides targeting the mRNA of the gap junction protein Cx43 promote tissue repair in a variety of different wounds. Delivery of the antisense drug has most often been achieved by a thermoreversible hydrogel, Pluronic F-127, which is very effective in the short term but does n...
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| Format: | Journal Article |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/83373 http://hdl.handle.net/10220/41421 |
| _version_ | 1824453516194742272 |
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| author | Gilmartin, Daniel J. Soon, Allyson Thrasivoulou, Christopher Phillips, Anthony R. J. Jayasinghe, Suwan N. Becker, David Lawrence |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Gilmartin, Daniel J. Soon, Allyson Thrasivoulou, Christopher Phillips, Anthony R. J. Jayasinghe, Suwan N. Becker, David Lawrence |
| author_sort | Gilmartin, Daniel J. |
| collection | NTU |
| description | Antisense oligodeoxynucleotides targeting the mRNA of the gap junction protein Cx43 promote tissue repair in a variety of different wounds. Delivery of the antisense drug has most often been achieved by a thermoreversible hydrogel, Pluronic F-127, which is very effective in the short term but does not allow for sustained delivery over several days. For chronic wounds that take a long time to heal, repeated dosing with the drug may be desirable but is not always compatible with conventional treatments such as the weekly changing of compression bandages on venous leg ulcers. Here the coating of collagen scaffolds with antisense oligonucleotides is investigated and a way to provide protection of the oligodeoxynucleotide drug is found in conjunction with sustained release over a 7 d period. This approach significantly reduces the normal foreign body reaction to the scaffold, which induces an increase of Cx43 protein and an inhibition of healing. As a result of the antisense integration into the scaffold, inflammation is reduced with the rate of wound healing and contracture is significantly improved. This coated scaffold approach may be very useful for treating venous leg ulcers and also for providing a sustained release of any other types of oligonucleotide drugs that are being developed. |
| first_indexed | 2025-02-19T03:07:39Z |
| format | Journal Article |
| id | ntu-10356/83373 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:07:39Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/833732021-03-10T02:50:20Z Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing Gilmartin, Daniel J. Soon, Allyson Thrasivoulou, Christopher Phillips, Anthony R. J. Jayasinghe, Suwan N. Becker, David Lawrence Lee Kong Chian School of Medicine (LKCMedicine) Lee Kong Chian School of Medicine Tissue Repair Oligonucleotide Delivery Antisense oligodeoxynucleotides targeting the mRNA of the gap junction protein Cx43 promote tissue repair in a variety of different wounds. Delivery of the antisense drug has most often been achieved by a thermoreversible hydrogel, Pluronic F-127, which is very effective in the short term but does not allow for sustained delivery over several days. For chronic wounds that take a long time to heal, repeated dosing with the drug may be desirable but is not always compatible with conventional treatments such as the weekly changing of compression bandages on venous leg ulcers. Here the coating of collagen scaffolds with antisense oligonucleotides is investigated and a way to provide protection of the oligodeoxynucleotide drug is found in conjunction with sustained release over a 7 d period. This approach significantly reduces the normal foreign body reaction to the scaffold, which induces an increase of Cx43 protein and an inhibition of healing. As a result of the antisense integration into the scaffold, inflammation is reduced with the rate of wound healing and contracture is significantly improved. This coated scaffold approach may be very useful for treating venous leg ulcers and also for providing a sustained release of any other types of oligonucleotide drugs that are being developed. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Accepted version 2016-09-06T05:37:38Z 2019-12-06T15:21:02Z 2016-09-06T05:37:38Z 2019-12-06T15:21:02Z 2016 Journal Article Gilmartin, D. J., Soon, A., Thrasivoulou, C., Phillips, A. R. J., Jayasinghe, S. N., & Becker, D. L. (2016). Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing. Advanced Healthcare Materials, 5(14), 1786-1799. 2192-2640 https://hdl.handle.net/10356/83373 http://hdl.handle.net/10220/41421 10.1002/adhm.201600175 en Advanced Healthcare Materials © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Advanced Healthcare Materials, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/adhm.201600175]. 35 p. application/pdf |
| spellingShingle | Tissue Repair Oligonucleotide Delivery Gilmartin, Daniel J. Soon, Allyson Thrasivoulou, Christopher Phillips, Anthony R. J. Jayasinghe, Suwan N. Becker, David Lawrence Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title | Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title_full | Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title_fullStr | Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title_full_unstemmed | Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title_short | Sustained Release of Cx43 Antisense Oligodeoxynucleotides from Coated Collagen Scaffolds Promotes Wound Healing |
| title_sort | sustained release of cx43 antisense oligodeoxynucleotides from coated collagen scaffolds promotes wound healing |
| topic | Tissue Repair Oligonucleotide Delivery |
| url | https://hdl.handle.net/10356/83373 http://hdl.handle.net/10220/41421 |
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