25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells
25-Hydroxyvitamin D3 [25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3 deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3 on osteogenic differentiatio...
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/83510 http://hdl.handle.net/10220/42643 |
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| author | Lou, Yan-Ru Toh, Tai Chong Tee, Yee Han Yu, Hanry |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lou, Yan-Ru Toh, Tai Chong Tee, Yee Han Yu, Hanry |
| author_sort | Lou, Yan-Ru |
| collection | NTU |
| description | 25-Hydroxyvitamin D3 [25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3 deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3 on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We also studied the effect of 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3], a metabolite of 25(OH)D3. One of the vitamin D responsive genes, 25(OH)D3-24-hydroxylase (cytochrome P450 family 24 subfamily A member 1) mRNA expression is up-regulated by 25(OH)D3 at 250–500 nM and by 1α,25-(OH)2D3 at 1–10 nM. 25(OH)D3 and 1α,25-(OH)2D3 at a time-dependent manner alter cell morphology towards osteoblast-associated characteristics. The osteogenic markers, alkaline phosphatase, secreted phosphoprotein 1 (osteopontin), and bone gamma-carboxyglutamate protein (osteocalcin) are increased by 25(OH)D3 and 1α,25-(OH)2D3 in a dose-dependent manner. Finally, mineralisation is significantly increased by 25(OH)D3 but not by 1α,25-(OH)2D3. Moreover, we found that hMSCs express very low level of 25(OH)D3-1α-hydroxylase (cytochrome P450 family 27 subfamily B member 1), and there is no detectable 1α,25-(OH)2D3 product. Taken together, our findings provide evidence that 25(OH)D3 at 250–500 nM can induce osteogenic differentiation and that 25(OH)D3 has great potential for cell-based bone tissue engineering. |
| first_indexed | 2024-10-01T04:43:42Z |
| format | Journal Article |
| id | ntu-10356/83510 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:43:42Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/835102023-02-28T16:59:04Z 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells Lou, Yan-Ru Toh, Tai Chong Tee, Yee Han Yu, Hanry School of Biological Sciences Mesenchymal stem cells Endocrine system and metabolic diseases 25-Hydroxyvitamin D3 [25(OH)D3] has recently been found to be an active hormone. Its biological actions are demonstrated in various cell types. 25(OH)D3 deficiency results in failure in bone formation and skeletal deformation. Here, we investigated the effect of 25(OH)D3 on osteogenic differentiation of human mesenchymal stem cells (hMSCs). We also studied the effect of 1α,25-dihydroxyvitamin D3 [1α,25-(OH)2D3], a metabolite of 25(OH)D3. One of the vitamin D responsive genes, 25(OH)D3-24-hydroxylase (cytochrome P450 family 24 subfamily A member 1) mRNA expression is up-regulated by 25(OH)D3 at 250–500 nM and by 1α,25-(OH)2D3 at 1–10 nM. 25(OH)D3 and 1α,25-(OH)2D3 at a time-dependent manner alter cell morphology towards osteoblast-associated characteristics. The osteogenic markers, alkaline phosphatase, secreted phosphoprotein 1 (osteopontin), and bone gamma-carboxyglutamate protein (osteocalcin) are increased by 25(OH)D3 and 1α,25-(OH)2D3 in a dose-dependent manner. Finally, mineralisation is significantly increased by 25(OH)D3 but not by 1α,25-(OH)2D3. Moreover, we found that hMSCs express very low level of 25(OH)D3-1α-hydroxylase (cytochrome P450 family 27 subfamily B member 1), and there is no detectable 1α,25-(OH)2D3 product. Taken together, our findings provide evidence that 25(OH)D3 at 250–500 nM can induce osteogenic differentiation and that 25(OH)D3 has great potential for cell-based bone tissue engineering. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2017-06-09T05:49:33Z 2019-12-06T15:24:32Z 2017-06-09T05:49:33Z 2019-12-06T15:24:32Z 2017 Journal Article Lou, Y.-R., Toh, T. C., Tee, Y. H., & Yu, H. (2017). 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells. Scientific Reports, 7, 42816-. 2045-2322 https://hdl.handle.net/10356/83510 http://hdl.handle.net/10220/42643 10.1038/srep42816 en Scientific Reports © 2017 The Author(s) (Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ 12 p. application/pdf |
| spellingShingle | Mesenchymal stem cells Endocrine system and metabolic diseases Lou, Yan-Ru Toh, Tai Chong Tee, Yee Han Yu, Hanry 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title | 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title_full | 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title_fullStr | 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title_full_unstemmed | 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title_short | 25-Hydroxyvitamin D3 induces osteogenic differentiation of human mesenchymal stem cells |
| title_sort | 25 hydroxyvitamin d3 induces osteogenic differentiation of human mesenchymal stem cells |
| topic | Mesenchymal stem cells Endocrine system and metabolic diseases |
| url | https://hdl.handle.net/10356/83510 http://hdl.handle.net/10220/42643 |
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