A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles
Cellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation o...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/83591 http://hdl.handle.net/10220/42663 |
| _version_ | 1826130311722827776 |
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| author | Gao, Yu Lim, Jing Yeo, David Chen Loong Liao, Shanshan Lans, Malin Wang, Yaqi Teoh, Swee-Hin Goh, Bee Tin Xu, Chenjie |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Gao, Yu Lim, Jing Yeo, David Chen Loong Liao, Shanshan Lans, Malin Wang, Yaqi Teoh, Swee-Hin Goh, Bee Tin Xu, Chenjie |
| author_sort | Gao, Yu |
| collection | NTU |
| description | Cellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation of cultured cells with the nanoparticles that eventually reach the intracellular compartment through specific or nonspecific internalization. This classic method is advantageous in terms of simplicity and convenience, but it suffers from issues such as difficulties in fully removing free nanoparticles (suspended in solution) and the lack of selectivity on cell types. This article reports an innovative strategy for the specific labeling of adherent cells without the concern of freely suspended nanoparticles. This method relies on a nanocomposite film that is prepared by homogeneously dispersing nanoparticles within a biodegradable polymeric film. When adherent cells are seeded on the film, they adhere, spread, and filtrate into the film through the micropores formed during the film fabrication. The pre-embedded nanoparticles are thus internalized by the cells during this infiltration process. As an example, fluorescent silica nanoparticles were homogeneously distributed within a polycaprolactone film by utilizing cryomilling and heat pressing. Upon incubation within physiological buffer, no silica nanoparticles were released from the nanocomposite film even after 20 d of incubation. However, when adherent cells (e.g., human mesenchymal stem cells) were grown on the film, they became fluorescent after 3 d, which suggests internalization of silica nanoparticles by cells. In comparison, the suspension cells (e.g., monocytes) in the medium remained nonfluorescent no matter whether there was the presence of adherent cells or not. This strategy eventually allowed the selective and concomitant labeling of mesenchymal stem cells during their harvest from bone marrow aspiration. |
| first_indexed | 2024-10-01T07:54:24Z |
| format | Journal Article |
| id | ntu-10356/83591 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:54:24Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/835912023-12-29T06:53:00Z A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles Gao, Yu Lim, Jing Yeo, David Chen Loong Liao, Shanshan Lans, Malin Wang, Yaqi Teoh, Swee-Hin Goh, Bee Tin Xu, Chenjie School of Chemical and Biomedical Engineering NTU-Northwestern Institute for Nanomedicine Selective cell labeling Nanoparticles Cellular labeling with inorganic nanoparticles such as magnetic iron oxide nanoparticles, quantum dots, and fluorescent silica nanoparticles is an important method for the noninvasive visualization of cells using various imaging modalities. Currently, this is mainly achieved through the incubation of cultured cells with the nanoparticles that eventually reach the intracellular compartment through specific or nonspecific internalization. This classic method is advantageous in terms of simplicity and convenience, but it suffers from issues such as difficulties in fully removing free nanoparticles (suspended in solution) and the lack of selectivity on cell types. This article reports an innovative strategy for the specific labeling of adherent cells without the concern of freely suspended nanoparticles. This method relies on a nanocomposite film that is prepared by homogeneously dispersing nanoparticles within a biodegradable polymeric film. When adherent cells are seeded on the film, they adhere, spread, and filtrate into the film through the micropores formed during the film fabrication. The pre-embedded nanoparticles are thus internalized by the cells during this infiltration process. As an example, fluorescent silica nanoparticles were homogeneously distributed within a polycaprolactone film by utilizing cryomilling and heat pressing. Upon incubation within physiological buffer, no silica nanoparticles were released from the nanocomposite film even after 20 d of incubation. However, when adherent cells (e.g., human mesenchymal stem cells) were grown on the film, they became fluorescent after 3 d, which suggests internalization of silica nanoparticles by cells. In comparison, the suspension cells (e.g., monocytes) in the medium remained nonfluorescent no matter whether there was the presence of adherent cells or not. This strategy eventually allowed the selective and concomitant labeling of mesenchymal stem cells during their harvest from bone marrow aspiration. MOE (Min. of Education, S’pore) Accepted version 2017-06-13T04:06:15Z 2019-12-06T15:26:17Z 2017-06-13T04:06:15Z 2019-12-06T15:26:17Z 2016 Journal Article Gao, Y., Lim, J., Yeo, D. C. L., Liao, S., Lans, M., Wang, Y., et al. (2016). A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles. ACS Applied Materials & Interfaces, 8(10), 6336-6343. 1944-8244 https://hdl.handle.net/10356/83591 http://hdl.handle.net/10220/42663 10.1021/acsami.5b12409 en ACS Applied Materials & Interfaces © 2016 American Chemical Society. This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Applied Materials & Interfaces, American Chemical Society. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acsami.5b12409]. 21 p. application/pdf |
| spellingShingle | Selective cell labeling Nanoparticles Gao, Yu Lim, Jing Yeo, David Chen Loong Liao, Shanshan Lans, Malin Wang, Yaqi Teoh, Swee-Hin Goh, Bee Tin Xu, Chenjie A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title | A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title_full | A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title_fullStr | A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title_full_unstemmed | A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title_short | A Selective and Purification-Free Strategy for Labeling Adherent Cells with Inorganic Nanoparticles |
| title_sort | selective and purification free strategy for labeling adherent cells with inorganic nanoparticles |
| topic | Selective cell labeling Nanoparticles |
| url | https://hdl.handle.net/10356/83591 http://hdl.handle.net/10220/42663 |
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