The gut microbiota influences blood-brain barrier permeability in mice
Pivotal to brain development and function is an intact blood-brain barrier (BBB), which acts as a gatekeeper to control the passage and exchange of molecules and nutrients between the circulatory system and the brain parenchyma. The BBB also ensures homeostasis of the central nervous system (CNS). W...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2016
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/83794 http://hdl.handle.net/10220/41486 |
| _version_ | 1826115932645228544 |
|---|---|
| author | Braniste, Viorica Al-Asmakh, Maha Kowal, Czeslawa Anuar, Farhana Abbaspour, Afrouz Tóth, Miklós Korecka, Agata Bakocevic, Nadja Ng, Lai Guan Kundu, Parag Gulyás, Balázs Halldin, Christer Hultenby, Kjell Nilsson, Harriet Hebert, Hans Volpe, Bruce T. Diamond, Betty Pettersson, Sven |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Braniste, Viorica Al-Asmakh, Maha Kowal, Czeslawa Anuar, Farhana Abbaspour, Afrouz Tóth, Miklós Korecka, Agata Bakocevic, Nadja Ng, Lai Guan Kundu, Parag Gulyás, Balázs Halldin, Christer Hultenby, Kjell Nilsson, Harriet Hebert, Hans Volpe, Bruce T. Diamond, Betty Pettersson, Sven |
| author_sort | Braniste, Viorica |
| collection | NTU |
| description | Pivotal to brain development and function is an intact blood-brain barrier (BBB), which acts as a gatekeeper to control the passage and exchange of molecules and nutrients between the circulatory system and the brain parenchyma. The BBB also ensures homeostasis of the central nervous system (CNS). We report that germ-free mice, beginning with intrauterine life, displayed increased BBB permeability compared to pathogen-free mice with a normal gut flora. The increased BBB permeability was maintained in germ-free mice after birth and during adulthood and was associated with reduced expression of the tight junction proteins occludin and claudin-5, which are known to regulate barrier function in endothelial tissues. Exposure of germ-free adult mice to a pathogen-free gut microbiota decreased BBB permeability and up-regulated the expression of tight junction proteins. Our results suggest that gut microbiota–BBB communication is initiated during gestation and propagated throughout life. |
| first_indexed | 2024-10-01T04:03:12Z |
| format | Journal Article |
| id | ntu-10356/83794 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:03:12Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/837942022-02-16T16:30:14Z The gut microbiota influences blood-brain barrier permeability in mice Braniste, Viorica Al-Asmakh, Maha Kowal, Czeslawa Anuar, Farhana Abbaspour, Afrouz Tóth, Miklós Korecka, Agata Bakocevic, Nadja Ng, Lai Guan Kundu, Parag Gulyás, Balázs Halldin, Christer Hultenby, Kjell Nilsson, Harriet Hebert, Hans Volpe, Bruce T. Diamond, Betty Pettersson, Sven Lee Kong Chian School of Medicine (LKCMedicine) Singapore Centre for Environmental Life Sciences Engineering Blood brain barrier Microbiota Pivotal to brain development and function is an intact blood-brain barrier (BBB), which acts as a gatekeeper to control the passage and exchange of molecules and nutrients between the circulatory system and the brain parenchyma. The BBB also ensures homeostasis of the central nervous system (CNS). We report that germ-free mice, beginning with intrauterine life, displayed increased BBB permeability compared to pathogen-free mice with a normal gut flora. The increased BBB permeability was maintained in germ-free mice after birth and during adulthood and was associated with reduced expression of the tight junction proteins occludin and claudin-5, which are known to regulate barrier function in endothelial tissues. Exposure of germ-free adult mice to a pathogen-free gut microbiota decreased BBB permeability and up-regulated the expression of tight junction proteins. Our results suggest that gut microbiota–BBB communication is initiated during gestation and propagated throughout life. ASTAR (Agency for Sci., Tech. and Research, S’pore) 2016-09-23T07:04:43Z 2019-12-06T15:32:12Z 2016-09-23T07:04:43Z 2019-12-06T15:32:12Z 2014 Journal Article Braniste, V., Al-Asmakh, M., Kowal, C., Anuar, F., Abbaspour, A., Toth, M., et al. (2014). The gut microbiota influences blood-brain barrier permeability in mice. Science Translational Medicine, 6(263), 263ra158-. 1946-6234 https://hdl.handle.net/10356/83794 http://hdl.handle.net/10220/41486 10.1126/scitranslmed.3009759 25411471 en Science Translational Medicine © 2014 American Association for the Advancement of Science. |
| spellingShingle | Blood brain barrier Microbiota Braniste, Viorica Al-Asmakh, Maha Kowal, Czeslawa Anuar, Farhana Abbaspour, Afrouz Tóth, Miklós Korecka, Agata Bakocevic, Nadja Ng, Lai Guan Kundu, Parag Gulyás, Balázs Halldin, Christer Hultenby, Kjell Nilsson, Harriet Hebert, Hans Volpe, Bruce T. Diamond, Betty Pettersson, Sven The gut microbiota influences blood-brain barrier permeability in mice |
| title | The gut microbiota influences blood-brain barrier permeability in mice |
| title_full | The gut microbiota influences blood-brain barrier permeability in mice |
| title_fullStr | The gut microbiota influences blood-brain barrier permeability in mice |
| title_full_unstemmed | The gut microbiota influences blood-brain barrier permeability in mice |
| title_short | The gut microbiota influences blood-brain barrier permeability in mice |
| title_sort | gut microbiota influences blood brain barrier permeability in mice |
| topic | Blood brain barrier Microbiota |
| url | https://hdl.handle.net/10356/83794 http://hdl.handle.net/10220/41486 |
| work_keys_str_mv | AT branisteviorica thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT alasmakhmaha thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT kowalczeslawa thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT anuarfarhana thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT abbaspourafrouz thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT tothmiklos thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT koreckaagata thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT bakocevicnadja thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT nglaiguan thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT kunduparag thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT gulyasbalazs thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT halldinchrister thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT hultenbykjell thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT nilssonharriet thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT heberthans thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT volpebrucet thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT diamondbetty thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT petterssonsven thegutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT branisteviorica gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT alasmakhmaha gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT kowalczeslawa gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT anuarfarhana gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT abbaspourafrouz gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT tothmiklos gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT koreckaagata gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT bakocevicnadja gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT nglaiguan gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT kunduparag gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT gulyasbalazs gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT halldinchrister gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT hultenbykjell gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT nilssonharriet gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT heberthans gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT volpebrucet gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT diamondbetty gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice AT petterssonsven gutmicrobiotainfluencesbloodbrainbarrierpermeabilityinmice |