Investigation into the Properties of Telomeric Mono-nucleosomes
One of the hallmark of eukaryotic cells is the presence of telomeres at the ends of their linear chromosomes. These sequences are highly conserved across different eukaryotic organisms, and in vertebrates, exist as repeats of (TTAGGG)n which would eventually end with a G-rich overhang [1]. Telomeres...
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| Format: | Student Research Poster |
| Language: | English |
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2016
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| Online Access: | https://hdl.handle.net/10356/84142 http://hdl.handle.net/10220/41623 |
| _version_ | 1811693655002447872 |
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| author | Tiu, Charles Kevin Dee |
| author2 | Lars Nordenskiold |
| author_facet | Lars Nordenskiold Tiu, Charles Kevin Dee |
| author_sort | Tiu, Charles Kevin Dee |
| collection | NTU |
| description | One of the hallmark of eukaryotic cells is the presence of telomeres at the ends of their linear chromosomes. These sequences are highly conserved across different eukaryotic organisms, and in vertebrates, exist as repeats of (TTAGGG)n which would eventually end with a G-rich overhang [1]. Telomeres are key in regulating genome stability, and their mis-regulation has been implicated in cancer and senescence [2]. Similar to non-telomeric regions of the genome, telomeres are wrapped around core histones, which, in turn, form the Nucleosome Core Particle (NCP). These NCPs further compacts and forms chromatin [3]. Core histones are multimeric proteins composed of 8 subunits (two of each: H2A, H2B, H3 and H4); and usually has a 147-bp DNA wrapped around this disc-shaped octamer. The structure and biophysics of telomere chromatin are not very well characterized [1]. This project aims to reconstitute telomeric DNA with human histone octamer in vitro, and use these reconstituted NCPs for structural and biophysical characterizations. In addition, large scale expression and purification of the telomeric DNA construct and the components of the histone octamer will be performed to produce samples for biophysical and structural characterizations. This poster reports preliminary work on the large scale expression and purification of human histone H2B (hH2B) and 147-bp telomeric DNA. [1st Award] |
| first_indexed | 2024-10-01T06:55:07Z |
| format | Student Research Poster |
| id | ntu-10356/84142 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:55:07Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | ntu-10356/841422020-09-27T20:29:57Z Investigation into the Properties of Telomeric Mono-nucleosomes Tiu, Charles Kevin Dee Lars Nordenskiold School of Biological Sciences Telomeres Nucleosomes One of the hallmark of eukaryotic cells is the presence of telomeres at the ends of their linear chromosomes. These sequences are highly conserved across different eukaryotic organisms, and in vertebrates, exist as repeats of (TTAGGG)n which would eventually end with a G-rich overhang [1]. Telomeres are key in regulating genome stability, and their mis-regulation has been implicated in cancer and senescence [2]. Similar to non-telomeric regions of the genome, telomeres are wrapped around core histones, which, in turn, form the Nucleosome Core Particle (NCP). These NCPs further compacts and forms chromatin [3]. Core histones are multimeric proteins composed of 8 subunits (two of each: H2A, H2B, H3 and H4); and usually has a 147-bp DNA wrapped around this disc-shaped octamer. The structure and biophysics of telomere chromatin are not very well characterized [1]. This project aims to reconstitute telomeric DNA with human histone octamer in vitro, and use these reconstituted NCPs for structural and biophysical characterizations. In addition, large scale expression and purification of the telomeric DNA construct and the components of the histone octamer will be performed to produce samples for biophysical and structural characterizations. This poster reports preliminary work on the large scale expression and purification of human histone H2B (hH2B) and 147-bp telomeric DNA. [1st Award] 2016-11-04T04:47:15Z 2019-12-06T15:39:12Z 2016-11-04T04:47:15Z 2019-12-06T15:39:12Z 2016 Student Research Poster Tiu, C. K. D. (2016, March). Investigation into the Properties of Telomeric Mono-nucleosomes. Presented at Discover URECA @ NTU poster exhibition and competition, Nanyang Technological University, Singapore. https://hdl.handle.net/10356/84142 http://hdl.handle.net/10220/41623 en © 2016 The Author(s). application/pdf |
| spellingShingle | Telomeres Nucleosomes Tiu, Charles Kevin Dee Investigation into the Properties of Telomeric Mono-nucleosomes |
| title | Investigation into the Properties of Telomeric Mono-nucleosomes |
| title_full | Investigation into the Properties of Telomeric Mono-nucleosomes |
| title_fullStr | Investigation into the Properties of Telomeric Mono-nucleosomes |
| title_full_unstemmed | Investigation into the Properties of Telomeric Mono-nucleosomes |
| title_short | Investigation into the Properties of Telomeric Mono-nucleosomes |
| title_sort | investigation into the properties of telomeric mono nucleosomes |
| topic | Telomeres Nucleosomes |
| url | https://hdl.handle.net/10356/84142 http://hdl.handle.net/10220/41623 |
| work_keys_str_mv | AT tiucharleskevindee investigationintothepropertiesoftelomericmononucleosomes |