Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma
Fluorescence microscopy has been widely used in characterizing the pathological states of tissues because intensity and spectra arise from fluorescence emission can reveal structural and biochemical information of biological samples and the fluorescence excited state lifetime has been verified to id...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Conference Paper |
| Language: | English |
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2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/84658 http://hdl.handle.net/10220/12170 |
| _version_ | 1826127154772967424 |
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| author | Jun, Gu. Yaw, Fu Chit. Koon, Ng Beng. Gulam Razul, Sirajudeen. Kim, Lim Soo. |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Jun, Gu. Yaw, Fu Chit. Koon, Ng Beng. Gulam Razul, Sirajudeen. Kim, Lim Soo. |
| author_sort | Jun, Gu. |
| collection | NTU |
| description | Fluorescence microscopy has been widely used in characterizing the pathological states of tissues because intensity and spectra arise from fluorescence emission can reveal structural and biochemical information of biological samples and the fluorescence excited state lifetime has been verified to identify tissue pathology due to its sensitivity to the fluorophore microenvironment. In this study, we have demonstrated that early cervical cancer can be quantitatively diagnosed using intensity and lifetime derived from the stroma fluorescence in conjunction with extreme learning machine (ELM) classifier which can result in a concurrently high sensitivity of 99.1% and specificity of 99.6%. The results suggest that the proposed technique can be used to aid and supplement the traditional histopathological examination of cervical precancer. |
| first_indexed | 2024-10-01T07:04:08Z |
| format | Conference Paper |
| id | ntu-10356/84658 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:04:08Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/846582020-03-07T13:24:45Z Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma Jun, Gu. Yaw, Fu Chit. Koon, Ng Beng. Gulam Razul, Sirajudeen. Kim, Lim Soo. School of Electrical and Electronic Engineering Photonics Global Conference (2012 : Singapore) DRNTU::Engineering::Electrical and electronic engineering Fluorescence microscopy has been widely used in characterizing the pathological states of tissues because intensity and spectra arise from fluorescence emission can reveal structural and biochemical information of biological samples and the fluorescence excited state lifetime has been verified to identify tissue pathology due to its sensitivity to the fluorophore microenvironment. In this study, we have demonstrated that early cervical cancer can be quantitatively diagnosed using intensity and lifetime derived from the stroma fluorescence in conjunction with extreme learning machine (ELM) classifier which can result in a concurrently high sensitivity of 99.1% and specificity of 99.6%. The results suggest that the proposed technique can be used to aid and supplement the traditional histopathological examination of cervical precancer. 2013-07-25T03:20:37Z 2019-12-06T15:49:00Z 2013-07-25T03:20:37Z 2019-12-06T15:49:00Z 2012 2012 Conference Paper Jun, G., Yaw, F. C., Koon, N. B., Gulam Razul, S., & Kim, L. S. (2012). Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma . 2012 Photonics Global Conference (PGC). https://hdl.handle.net/10356/84658 http://hdl.handle.net/10220/12170 10.1109/PGC.2012.6458087 en © 2012 IEEE. |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering Jun, Gu. Yaw, Fu Chit. Koon, Ng Beng. Gulam Razul, Sirajudeen. Kim, Lim Soo. Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title | Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title_full | Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title_fullStr | Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title_full_unstemmed | Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title_short | Quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| title_sort | quantitative diagnosis of cervical precancer using fluorescence intensity and lifetime imaging from the stroma |
| topic | DRNTU::Engineering::Electrical and electronic engineering |
| url | https://hdl.handle.net/10356/84658 http://hdl.handle.net/10220/12170 |
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