Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA
Non-invasive and longitudinal monitoring of gene expression in living cells is essential for understanding and monitoring cellular activities. Herein, a smart magnetic nanosensor is constructed for the real-time, noninvasive, and longitudinal monitoring of cellular mRNA expression through the layer-...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2017
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| Online Access: | https://hdl.handle.net/10356/84823 http://hdl.handle.net/10220/42016 |
| _version_ | 1826115531595317248 |
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| author | Xu, Zhichuan Jason Wang, Min Hou, Xiaochun Wiraja, Christian Sun, Libo Xu, Chenjie |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Xu, Zhichuan Jason Wang, Min Hou, Xiaochun Wiraja, Christian Sun, Libo Xu, Chenjie |
| author_sort | Xu, Zhichuan Jason |
| collection | NTU |
| description | Non-invasive and longitudinal monitoring of gene expression in living cells is essential for understanding and monitoring cellular activities. Herein, a smart magnetic nanosensor is constructed for the real-time, noninvasive, and longitudinal monitoring of cellular mRNA expression through the layer-by-layer deposition of molecular beacons (MBs) and polyethylenimine on the iron oxide nanoparticles. The loading of MBs, responsible for the signal intensity and the tracking time, was easily tuned with the number of layers incorporated. The idea was first demonstrated with the magnetic nanosensors for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, which was efficiently internalized into the cells under the influence of magnetic field. This nanosensor allowed the continuous monitoring of the cellular GAPDH mRNA expression for 1 month. Then this platform was further utilized to incorporate two kinds of MBs for alkaline phosphatase (ALP) and GAPDH mRNAs, respectively. The multifunctional nanosensors permitted the simultaneous monitoring of the reference gene (GAPDH mRNA) and the early osteogenic differentiation marker (ALP mRNA) expression. When the fluorescence signal ratio between ALP mRNA MBs and GAPDH mRNA MBs was taken, the dynamic osteogenic differentiation process of MSCs was accurately monitored. |
| first_indexed | 2024-10-01T03:56:41Z |
| format | Journal Article |
| id | ntu-10356/84823 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:56:41Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/848232023-12-29T06:47:12Z Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA Xu, Zhichuan Jason Wang, Min Hou, Xiaochun Wiraja, Christian Sun, Libo Xu, Chenjie School of Chemical and Biomedical Engineering School of Materials Science & Engineering NTU-Northwestern Institute for Nanomedicine Magnetic nanoparticle Nanosensor Non-invasive and longitudinal monitoring of gene expression in living cells is essential for understanding and monitoring cellular activities. Herein, a smart magnetic nanosensor is constructed for the real-time, noninvasive, and longitudinal monitoring of cellular mRNA expression through the layer-by-layer deposition of molecular beacons (MBs) and polyethylenimine on the iron oxide nanoparticles. The loading of MBs, responsible for the signal intensity and the tracking time, was easily tuned with the number of layers incorporated. The idea was first demonstrated with the magnetic nanosensors for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA, which was efficiently internalized into the cells under the influence of magnetic field. This nanosensor allowed the continuous monitoring of the cellular GAPDH mRNA expression for 1 month. Then this platform was further utilized to incorporate two kinds of MBs for alkaline phosphatase (ALP) and GAPDH mRNAs, respectively. The multifunctional nanosensors permitted the simultaneous monitoring of the reference gene (GAPDH mRNA) and the early osteogenic differentiation marker (ALP mRNA) expression. When the fluorescence signal ratio between ALP mRNA MBs and GAPDH mRNA MBs was taken, the dynamic osteogenic differentiation process of MSCs was accurately monitored. Accepted version 2017-01-13T08:21:43Z 2019-12-06T15:51:41Z 2017-01-13T08:21:43Z 2019-12-06T15:51:41Z 2016 Journal Article Wang, M., Hou, X., Wiraja, C., Sun, L., Xu, Z. J., & Xu, C. (2016). Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA. ACS Applied Materials & Interfaces, 8(9), 5877-5886. 1944-8244 https://hdl.handle.net/10356/84823 http://hdl.handle.net/10220/42016 10.1021/acsami.5b12234 en ACS Applied Materials & Interfaces © 2016 American Chemical Society (ACS). This is the author created version of a work that has been peer reviewed and accepted for publication by ACS Applied Materials & Interfaces, American Chemical Society (ACS). It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1021/acsami.5b12234]. 31 p. application/pdf |
| spellingShingle | Magnetic nanoparticle Nanosensor Xu, Zhichuan Jason Wang, Min Hou, Xiaochun Wiraja, Christian Sun, Libo Xu, Chenjie Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title | Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title_full | Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title_fullStr | Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title_full_unstemmed | Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title_short | Smart Magnetic Nanosensors Synthesized through Layer-by-Layer Deposition of Molecular Beacons for Noninvasive and Longitudinal Monitoring of Cellular mRNA |
| title_sort | smart magnetic nanosensors synthesized through layer by layer deposition of molecular beacons for noninvasive and longitudinal monitoring of cellular mrna |
| topic | Magnetic nanoparticle Nanosensor |
| url | https://hdl.handle.net/10356/84823 http://hdl.handle.net/10220/42016 |
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