Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity
Profilin functions with formin in actin assembly, a process that regulates multiple aspects of plant development and immune responses. High-level eukaryotes contain multiple isoforms of profilin, formin, and actin, whose partner-specific interactions in actin assembly are not completely understood i...
| Main Authors: | , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/84886 http://hdl.handle.net/10220/49174 |
| _version_ | 1811680940604260352 |
|---|---|
| author | Sun, He Qiao, Zhu Chua, Khi Pin Tursic, Alma Liu, Xu Gao, Yong-Gui Mu, Yuguang Hou, Xingliang Miao, Yansong |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Sun, He Qiao, Zhu Chua, Khi Pin Tursic, Alma Liu, Xu Gao, Yong-Gui Mu, Yuguang Hou, Xingliang Miao, Yansong |
| author_sort | Sun, He |
| collection | NTU |
| description | Profilin functions with formin in actin assembly, a process that regulates multiple aspects of plant development and immune responses. High-level eukaryotes contain multiple isoforms of profilin, formin, and actin, whose partner-specific interactions in actin assembly are not completely understood in plant development and defense responses. To examine the functionally distinct interactions between profilin and formin, we studied all five Arabidopsis profilins and their interactions with formin by using both in vitro biochemical and in vivo cell biology approaches. Unexpectedly, we found a previously undescribed negative regulatory function of AtPRF3 in AtFH1-mediated actin polymerization. The N-terminal 37 residues of AtPRF3 were identified to play a predominant role in inhibiting formin-mediated actin nucleation via their high affinity for the formin polyproline region and their triggering of the oligomerization of AtPRF3. Both in vivo and in vitro mechanistic studies of AtPRF3 revealed a universal mechanism in which the weak interaction between profilin and formin positively regulates actin assembly by ensuring rapid recycling of profilin, whereas profilin oligomerization negatively regulates actin polymerization. Upon recognition of the pathogen-associated molecular pattern, the gene transcription and protein degradation of AtPRF3 are modulated for actin assembly during plant innate immunity. The prf3 Arabidopsis plants show higher sensitivity to the bacterial flagellum peptide in both the plant growth and ROS responses. These findings demonstrate a profilin-mediated actin assembly mechanism underlying the plant immune responses. |
| first_indexed | 2024-10-01T03:33:02Z |
| format | Journal Article |
| id | ntu-10356/84886 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:33:02Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/848862020-11-01T04:44:29Z Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity Sun, He Qiao, Zhu Chua, Khi Pin Tursic, Alma Liu, Xu Gao, Yong-Gui Mu, Yuguang Hou, Xingliang Miao, Yansong School of Chemical and Biomedical Engineering School of Biological Sciences Interdisciplinary Graduate School (IGS) Science::Biological sciences Actin Cytoskeleton Profilin Profilin functions with formin in actin assembly, a process that regulates multiple aspects of plant development and immune responses. High-level eukaryotes contain multiple isoforms of profilin, formin, and actin, whose partner-specific interactions in actin assembly are not completely understood in plant development and defense responses. To examine the functionally distinct interactions between profilin and formin, we studied all five Arabidopsis profilins and their interactions with formin by using both in vitro biochemical and in vivo cell biology approaches. Unexpectedly, we found a previously undescribed negative regulatory function of AtPRF3 in AtFH1-mediated actin polymerization. The N-terminal 37 residues of AtPRF3 were identified to play a predominant role in inhibiting formin-mediated actin nucleation via their high affinity for the formin polyproline region and their triggering of the oligomerization of AtPRF3. Both in vivo and in vitro mechanistic studies of AtPRF3 revealed a universal mechanism in which the weak interaction between profilin and formin positively regulates actin assembly by ensuring rapid recycling of profilin, whereas profilin oligomerization negatively regulates actin polymerization. Upon recognition of the pathogen-associated molecular pattern, the gene transcription and protein degradation of AtPRF3 are modulated for actin assembly during plant innate immunity. The prf3 Arabidopsis plants show higher sensitivity to the bacterial flagellum peptide in both the plant growth and ROS responses. These findings demonstrate a profilin-mediated actin assembly mechanism underlying the plant immune responses. MOE (Min. of Education, S’pore) Accepted version 2019-07-08T04:45:35Z 2019-12-06T15:53:02Z 2019-07-08T04:45:35Z 2019-12-06T15:53:02Z 2018 Journal Article Sun, H., Qiao, Z., Chua, K. P., Tursic, A., Liu, X., Gao, Y.-G., . . . Miao, Y. (2018). Profilin Negatively Regulates Formin-Mediated Actin Assembly to Modulate PAMP-Triggered Plant Immunity. Current Biology, 28(12), 1882-1895. doi:10.1016/j.cub.2018.04.045 0960-9822 https://hdl.handle.net/10356/84886 http://hdl.handle.net/10220/49174 10.1016/j.cub.2018.04.045 en Current Biology © 2018 Elsevier Ltd. All rights reserved. This paper was published in Current Biology and is made available with permission of Elsevier Ltd. 25 p. application/pdf |
| spellingShingle | Science::Biological sciences Actin Cytoskeleton Profilin Sun, He Qiao, Zhu Chua, Khi Pin Tursic, Alma Liu, Xu Gao, Yong-Gui Mu, Yuguang Hou, Xingliang Miao, Yansong Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title | Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title_full | Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title_fullStr | Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title_full_unstemmed | Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title_short | Profilin negatively regulates formin-mediated actin assembly to modulate pamp-triggered plant immunity |
| title_sort | profilin negatively regulates formin mediated actin assembly to modulate pamp triggered plant immunity |
| topic | Science::Biological sciences Actin Cytoskeleton Profilin |
| url | https://hdl.handle.net/10356/84886 http://hdl.handle.net/10220/49174 |
| work_keys_str_mv | AT sunhe profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT qiaozhu profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT chuakhipin profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT tursicalma profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT liuxu profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT gaoyonggui profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT muyuguang profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT houxingliang profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity AT miaoyansong profilinnegativelyregulatesforminmediatedactinassemblytomodulatepamptriggeredplantimmunity |