Investigation of the microbial communities colonizing prepainted steel used for roofing and walling
Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2017
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/84996 http://hdl.handle.net/10220/42093 |
| _version_ | 1826112559788326912 |
|---|---|
| author | Huynh, Tran T. Jamil, Ili Pianegonda, Nicole A. Blanksby, Stephen J. Barker, Philip J. Manefield, Mike Rice, Scott A. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Huynh, Tran T. Jamil, Ili Pianegonda, Nicole A. Blanksby, Stephen J. Barker, Philip J. Manefield, Mike Rice, Scott A. |
| author_sort | Huynh, Tran T. |
| collection | NTU |
| description | Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design fouling prevention strategies. Here, next-generation sequencing and microbial community finger printing (T-RFLP) were used to study the community composition of microbes colonizing prepainted steel roofing materials at Burrawang, Australia and Kapar, Malaysia over a 52-week period. Community diversity was low and was dominated by Bacillus spp., cyanobacteria, actinobacteria, Cladosporium sp., Epicoccum nigrum, and Teratosphaeriaceae sp. Cultivation-based methods isolated approximately 20 different fungi and bacteria, some of which, such as E. nigrum and Cladosporium sp., were represented in the community sequence data. Fluorescence in situ hybridization imaging showed that fungi were the most dominant organisms present. Analysis of the sequence and T-RFLP data indicated that the microbial communities differed significantly between locations and changed significantly over time. The study demonstrates the utility of molecular ecology tools to identify and characterize microbial communities associated with the fouling of painted steel surfaces and ultimately can enable the targeted development of control strategies based on the dominant species responsible for fouling. |
| first_indexed | 2024-10-01T03:09:03Z |
| format | Journal Article |
| id | ntu-10356/84996 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:09:03Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/849962020-09-21T11:32:21Z Investigation of the microbial communities colonizing prepainted steel used for roofing and walling Huynh, Tran T. Jamil, Ili Pianegonda, Nicole A. Blanksby, Stephen J. Barker, Philip J. Manefield, Mike Rice, Scott A. School of Biological Sciences Singapore Centre for Environmental Life Sciences Engineering Biofilms Biofouling Microbial colonization of prepainted steel, commonly used in roofing applications, impacts their aesthetics, durability, and functionality. Understanding the relevant organisms and the mechanisms by which colonization occurs would provide valuable information that can be subsequently used to design fouling prevention strategies. Here, next-generation sequencing and microbial community finger printing (T-RFLP) were used to study the community composition of microbes colonizing prepainted steel roofing materials at Burrawang, Australia and Kapar, Malaysia over a 52-week period. Community diversity was low and was dominated by Bacillus spp., cyanobacteria, actinobacteria, Cladosporium sp., Epicoccum nigrum, and Teratosphaeriaceae sp. Cultivation-based methods isolated approximately 20 different fungi and bacteria, some of which, such as E. nigrum and Cladosporium sp., were represented in the community sequence data. Fluorescence in situ hybridization imaging showed that fungi were the most dominant organisms present. Analysis of the sequence and T-RFLP data indicated that the microbial communities differed significantly between locations and changed significantly over time. The study demonstrates the utility of molecular ecology tools to identify and characterize microbial communities associated with the fouling of painted steel surfaces and ultimately can enable the targeted development of control strategies based on the dominant species responsible for fouling. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Published version 2017-02-16T05:54:33Z 2019-12-06T15:55:08Z 2017-02-16T05:54:33Z 2019-12-06T15:55:08Z 2016 Journal Article Huynh, T. T., Jamil, I., Pianegonda, N. A., Blanksby, S. J., Barker, P. J., Manefield, M., et al. (2016). Investigation of the microbial communities colonizing prepainted steel used for roofing and walling. MicrobiologyOpen, 6(2), e00425-. https://hdl.handle.net/10356/84996 http://hdl.handle.net/10220/42093 10.1002/mbo3.425 en MicrobiologyOpen © 2016 John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium provided the original work is properly cited. 11 p. application/pdf |
| spellingShingle | Biofilms Biofouling Huynh, Tran T. Jamil, Ili Pianegonda, Nicole A. Blanksby, Stephen J. Barker, Philip J. Manefield, Mike Rice, Scott A. Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title | Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title_full | Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title_fullStr | Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title_full_unstemmed | Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title_short | Investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| title_sort | investigation of the microbial communities colonizing prepainted steel used for roofing and walling |
| topic | Biofilms Biofouling |
| url | https://hdl.handle.net/10356/84996 http://hdl.handle.net/10220/42093 |
| work_keys_str_mv | AT huynhtrant investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT jamilili investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT pianegondanicolea investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT blanksbystephenj investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT barkerphilipj investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT manefieldmike investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling AT ricescotta investigationofthemicrobialcommunitiescolonizingprepaintedsteelusedforroofingandwalling |