Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation
Bacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/85341 http://hdl.handle.net/10220/49804 |
| _version_ | 1811691916749701120 |
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| author | Riau, Andri Kartasasmita Aung, Thet Tun Setiawan, Melina Yang, Liang Yam, Gary Hin Fai Beuerman, Roger W. Venkatraman, Subbu Subramanian Mehta, Jodhbir Singh |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Riau, Andri Kartasasmita Aung, Thet Tun Setiawan, Melina Yang, Liang Yam, Gary Hin Fai Beuerman, Roger W. Venkatraman, Subbu Subramanian Mehta, Jodhbir Singh |
| author_sort | Riau, Andri Kartasasmita |
| collection | NTU |
| description | Bacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature dissolution, particularly in harsh diseased tissue microenvironment, leading to rapid nAg clearance. It stands to reason that impregnating nAg directly onto the device, at the surface, is a more ideal solution. We tested this concept for a corneal prosthesis by immobilizing nAg and nano-hydroxyapatite (nHAp) on poly(methyl methacrylate), and tested its biocompatibility with human stromal cells and antimicrobial performance against biofilm-forming pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Three different dual-functionalized substrates—high Ag (referred to as 75:25 HAp:Ag); intermediate Ag (95:5 HAp:Ag); and low Ag (99:1 HAp:Ag) were studied. The 75:25 HAp:Ag was effective in inhibiting biofilm formation, but was cytotoxic. The 95:5 HAp:Ag showed the best selectivity among the three substrates; it prevented biofilm formation of both pathogens and had excellent biocompatibility. The coating was also effective in eliminating non-adherent bacteria in the culture media. However, a 28-day incubation in artificial tear fluid revealed a ~40% reduction in Ag+ release, compared to freshly-coated substrates. The reduction affected the inhibition of S. aureus growth, but not the P. aeruginosa. Our findings suggest that Ag+ released from surface-immobilized nAg diminishes over time and becomes less effective in suppressing biofilm formation of Gram-positive bacteria, such as S. aureus. This advocates the coating, more as a protection against perioperative and early postoperative infections, and less as a long-term preventive solution. |
| first_indexed | 2024-10-01T06:27:30Z |
| format | Journal Article |
| id | ntu-10356/85341 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:27:30Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/853412023-07-14T15:50:54Z Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation Riau, Andri Kartasasmita Aung, Thet Tun Setiawan, Melina Yang, Liang Yam, Gary Hin Fai Beuerman, Roger W. Venkatraman, Subbu Subramanian Mehta, Jodhbir Singh School of Materials Science & Engineering Engineering::Materials Silver Toxicity Bacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature dissolution, particularly in harsh diseased tissue microenvironment, leading to rapid nAg clearance. It stands to reason that impregnating nAg directly onto the device, at the surface, is a more ideal solution. We tested this concept for a corneal prosthesis by immobilizing nAg and nano-hydroxyapatite (nHAp) on poly(methyl methacrylate), and tested its biocompatibility with human stromal cells and antimicrobial performance against biofilm-forming pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Three different dual-functionalized substrates—high Ag (referred to as 75:25 HAp:Ag); intermediate Ag (95:5 HAp:Ag); and low Ag (99:1 HAp:Ag) were studied. The 75:25 HAp:Ag was effective in inhibiting biofilm formation, but was cytotoxic. The 95:5 HAp:Ag showed the best selectivity among the three substrates; it prevented biofilm formation of both pathogens and had excellent biocompatibility. The coating was also effective in eliminating non-adherent bacteria in the culture media. However, a 28-day incubation in artificial tear fluid revealed a ~40% reduction in Ag+ release, compared to freshly-coated substrates. The reduction affected the inhibition of S. aureus growth, but not the P. aeruginosa. Our findings suggest that Ag+ released from surface-immobilized nAg diminishes over time and becomes less effective in suppressing biofilm formation of Gram-positive bacteria, such as S. aureus. This advocates the coating, more as a protection against perioperative and early postoperative infections, and less as a long-term preventive solution. Published version 2019-08-28T01:53:58Z 2019-12-06T16:01:58Z 2019-08-28T01:53:58Z 2019-12-06T16:01:58Z 2019 Journal Article Riau, A. K., Aung, T. T., Setiawan, M., Yang, L., Yam, G. H. F., Beuerman, R. W., . . . Mehta, S. S. (2019). Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation. Pathogens, 8(3), 93-. doi:10.3390/pathogens8030093 2076-0817 https://hdl.handle.net/10356/85341 http://hdl.handle.net/10220/49804 10.3390/pathogens8030093 en Pathogens © 2019 by the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). 17 p. application/pdf |
| spellingShingle | Engineering::Materials Silver Toxicity Riau, Andri Kartasasmita Aung, Thet Tun Setiawan, Melina Yang, Liang Yam, Gary Hin Fai Beuerman, Roger W. Venkatraman, Subbu Subramanian Mehta, Jodhbir Singh Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title | Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title_full | Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title_fullStr | Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title_full_unstemmed | Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title_short | Surface immobilization of nano-silver on polymeric medical devices to prevent bacterial biofilm formation |
| title_sort | surface immobilization of nano silver on polymeric medical devices to prevent bacterial biofilm formation |
| topic | Engineering::Materials Silver Toxicity |
| url | https://hdl.handle.net/10356/85341 http://hdl.handle.net/10220/49804 |
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