Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex
Objective: To define the physiological properties of neurons projecting from the claustrum to the anterior cingulate cortex (ACC). Design: To identify the claustrum in live slices, we used a transgenic mouse line that expresses yellow fluorescent protein (YFP)-tagged Volvox channelrhodopsin-1 at...
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/85591 http://hdl.handle.net/10220/43764 |
| _version_ | 1811682449561747456 |
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| author | Chia, Zach Silberberg, Gilad Augustine, George James |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Chia, Zach Silberberg, Gilad Augustine, George James |
| author_sort | Chia, Zach |
| collection | NTU |
| description | Objective: To define the physiological properties of neurons projecting from the claustrum to the anterior cingulate cortex (ACC).
Design: To identify the claustrum in live slices, we used a transgenic mouse line that expresses yellow fluorescent protein (YFP)-tagged Volvox channelrhodopsin-1 at high levels within the claustrum. Claustrum cells projecting to the ACC were identified by retrograde labelling. Whole-cell patch-clamp recordings from labelled claustrum neurons were used to characterize the intrinsic electrical properties of these neurons. Cells were classified according to their intrinsic electrical properties, based on a previous classification scheme.
Results: Labelled neurons were found in the claustrum but not the insular cortex. Four types of ACC-projecting neurons were identified based on action potential adaptation and waveform: strongly adapting (SA) cell types 2, 3 and 4, and moderately adapting (MA) cell type 2. Labelled cells were predominantly SA4 in the anterior (44%) and posterior (63%) claustrum, while MA2 predominated (77%) in the central claustrum. The male anterior claustrum showed a bias toward SA3 cells (53%) while the female anterior claustrum showed a bias toward SA3 cells (76%).
Conclusions: There is ipsilateral dominance for ACC-projecting claustrum neurons, with the intrinsic properties of these neurons varying along the anterior–posterior axis. Sexual dimorphism was observed in ACC-projecting claustrum cells. Our results are consistent with the hypothesis that the claustrum serves as a link between the insular cortex and the ACC. |
| first_indexed | 2024-10-01T03:57:01Z |
| format | Journal Article |
| id | ntu-10356/85591 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:57:01Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/855912020-11-01T05:15:53Z Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex Chia, Zach Silberberg, Gilad Augustine, George James Lee Kong Chian School of Medicine (LKCMedicine) School of Biological Sciences Anterior cingulate cortex Claustrum Objective: To define the physiological properties of neurons projecting from the claustrum to the anterior cingulate cortex (ACC). Design: To identify the claustrum in live slices, we used a transgenic mouse line that expresses yellow fluorescent protein (YFP)-tagged Volvox channelrhodopsin-1 at high levels within the claustrum. Claustrum cells projecting to the ACC were identified by retrograde labelling. Whole-cell patch-clamp recordings from labelled claustrum neurons were used to characterize the intrinsic electrical properties of these neurons. Cells were classified according to their intrinsic electrical properties, based on a previous classification scheme. Results: Labelled neurons were found in the claustrum but not the insular cortex. Four types of ACC-projecting neurons were identified based on action potential adaptation and waveform: strongly adapting (SA) cell types 2, 3 and 4, and moderately adapting (MA) cell type 2. Labelled cells were predominantly SA4 in the anterior (44%) and posterior (63%) claustrum, while MA2 predominated (77%) in the central claustrum. The male anterior claustrum showed a bias toward SA3 cells (53%) while the female anterior claustrum showed a bias toward SA3 cells (76%). Conclusions: There is ipsilateral dominance for ACC-projecting claustrum neurons, with the intrinsic properties of these neurons varying along the anterior–posterior axis. Sexual dimorphism was observed in ACC-projecting claustrum cells. Our results are consistent with the hypothesis that the claustrum serves as a link between the insular cortex and the ACC. MOE (Min. of Education, S’pore) Published version 2017-09-19T03:23:19Z 2019-12-06T16:06:44Z 2017-09-19T03:23:19Z 2019-12-06T16:06:44Z 2017 Journal Article Chia, Z., Silberberg, G., & Augustine, G. J. (2017). Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex. Claustrum, 2(1), 1357412-. https://hdl.handle.net/10356/85591 http://hdl.handle.net/10220/43764 10.1080/20023294.2017.1357412 en Claustrum © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 13 p. application/pdf |
| spellingShingle | Anterior cingulate cortex Claustrum Chia, Zach Silberberg, Gilad Augustine, George James Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title | Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title_full | Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title_fullStr | Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title_full_unstemmed | Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title_short | Functional properties, topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| title_sort | functional properties topological organization and sexual dimorphism of claustrum neurons projecting to anterior cingulate cortex |
| topic | Anterior cingulate cortex Claustrum |
| url | https://hdl.handle.net/10356/85591 http://hdl.handle.net/10220/43764 |
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