Rapid generation of chemical combinations on a magnetic digital microfluidic array
Combinatorial screening is frequently used to identify chemicals with synergistic effects by measuring the response of biological entities exposed to various chemical-dose combinations. Conventional microwell-based combinatorial screening is resource-demanding, and the closed microfluidics-based scr...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2019
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| Online Access: | https://hdl.handle.net/10356/85713 http://hdl.handle.net/10220/49836 |
| _version_ | 1826110239310610432 |
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| author | Zhang, Yi Wang, Tza-Huei |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Zhang, Yi Wang, Tza-Huei |
| author_sort | Zhang, Yi |
| collection | NTU |
| description | Combinatorial screening is frequently used to identify chemicals with synergistic effects by measuring the response of biological entities exposed to various chemical-dose combinations. Conventional microwell-based combinatorial screening is resource-demanding, and the closed microfluidics-based screening requires sophisticated fluidic control systems. In this work, we present a novel combinatorial screening platform based on the surface energy trap (SET)-assisted magnetic digital microfluidics. This platform, known as FlipDrop, rapidly generates chemical combinations by coupling two droplet arrays with orthogonal chemical concentration gradients with a simple flip. We have illustrated the working principle of FlipDrop by generating combinations of quantum dots. We have also successfully demonstrated the screening of quantum dot fluorescence resonance energy transfer (QD-FRET) on the FlipDrop platform by measuring the FRET response. This report demonstrates that FlipDrop is capable of rapidly generating chemical combinations with unprecedented ease for combinatorial screening. |
| first_indexed | 2024-10-01T02:31:08Z |
| format | Journal Article |
| id | ntu-10356/85713 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:31:08Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/857132023-03-04T17:12:11Z Rapid generation of chemical combinations on a magnetic digital microfluidic array Zhang, Yi Wang, Tza-Huei School of Mechanical and Aerospace Engineering Microfluidic Chemical Combination Engineering::Mechanical engineering Combinatorial screening is frequently used to identify chemicals with synergistic effects by measuring the response of biological entities exposed to various chemical-dose combinations. Conventional microwell-based combinatorial screening is resource-demanding, and the closed microfluidics-based screening requires sophisticated fluidic control systems. In this work, we present a novel combinatorial screening platform based on the surface energy trap (SET)-assisted magnetic digital microfluidics. This platform, known as FlipDrop, rapidly generates chemical combinations by coupling two droplet arrays with orthogonal chemical concentration gradients with a simple flip. We have illustrated the working principle of FlipDrop by generating combinations of quantum dots. We have also successfully demonstrated the screening of quantum dot fluorescence resonance energy transfer (QD-FRET) on the FlipDrop platform by measuring the FRET response. This report demonstrates that FlipDrop is capable of rapidly generating chemical combinations with unprecedented ease for combinatorial screening. MOE (Min. of Education, S’pore) Published version 2019-09-02T04:52:53Z 2019-12-06T16:08:53Z 2019-09-02T04:52:53Z 2019-12-06T16:08:53Z 2019 Journal Article Zhang, Y., & Wang, T.-H. (2019). Rapid generation of chemical combinations on a magnetic digital microfluidic array. RSC Advances, 9(38), 21741-21747. doi:10.1039/C9RA03469B https://hdl.handle.net/10356/85713 http://hdl.handle.net/10220/49836 10.1039/C9RA03469B en RSC Advances © 2019 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. 7 p. application/pdf |
| spellingShingle | Microfluidic Chemical Combination Engineering::Mechanical engineering Zhang, Yi Wang, Tza-Huei Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title | Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title_full | Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title_fullStr | Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title_full_unstemmed | Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title_short | Rapid generation of chemical combinations on a magnetic digital microfluidic array |
| title_sort | rapid generation of chemical combinations on a magnetic digital microfluidic array |
| topic | Microfluidic Chemical Combination Engineering::Mechanical engineering |
| url | https://hdl.handle.net/10356/85713 http://hdl.handle.net/10220/49836 |
| work_keys_str_mv | AT zhangyi rapidgenerationofchemicalcombinationsonamagneticdigitalmicrofluidicarray AT wangtzahuei rapidgenerationofchemicalcombinationsonamagneticdigitalmicrofluidicarray |