Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte

One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemil...

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Main Authors: Ye, Kehan, Sinawang, Prima Dewi, Tok, Alfred Iing Yoong, Marks, Robert S.
Other Authors: School of Materials Science & Engineering
Format: Journal Article
Language:English
Published: 2019
Subjects:
Online Access:https://hdl.handle.net/10356/85760
http://hdl.handle.net/10220/49245
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author Ye, Kehan
Sinawang, Prima Dewi
Tok, Alfred Iing Yoong
Marks, Robert S.
author2 School of Materials Science & Engineering
author_facet School of Materials Science & Engineering
Ye, Kehan
Sinawang, Prima Dewi
Tok, Alfred Iing Yoong
Marks, Robert S.
author_sort Ye, Kehan
collection NTU
description One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. After optimization, it has shown superior adaptability over traditional chemiluminescent ELISA and a commonly used glutaraldehyde chemical immobilization method in detection limit (6.44 * 102 CFU/ml) and dynamic range (6.44 *105 to 6.44 *108 CFU/ml, r2 = 0.98; 6.44 *102 to 6.44 *105, r2 = 0.92). The newly developed immobilization method has enhanced the function of FOBS as an affordable and dispatchable tool in the early warning monitoring of the putative presence of E. coli cells. It can be used either in individual sample screening or in continuous monitoring of the environment to indicate the need for further investigation. The diazirine crosslinking method can also be adopted in the immobilization of other protein-based bioentities thus expanding its real-world utility.
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spelling ntu-10356/857602020-06-01T10:21:16Z Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte Ye, Kehan Sinawang, Prima Dewi Tok, Alfred Iing Yoong Marks, Robert S. School of Materials Science & Engineering Diazirine Silane Photoinducible Crosslinker Engineering::Materials One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. After optimization, it has shown superior adaptability over traditional chemiluminescent ELISA and a commonly used glutaraldehyde chemical immobilization method in detection limit (6.44 * 102 CFU/ml) and dynamic range (6.44 *105 to 6.44 *108 CFU/ml, r2 = 0.98; 6.44 *102 to 6.44 *105, r2 = 0.92). The newly developed immobilization method has enhanced the function of FOBS as an affordable and dispatchable tool in the early warning monitoring of the putative presence of E. coli cells. It can be used either in individual sample screening or in continuous monitoring of the environment to indicate the need for further investigation. The diazirine crosslinking method can also be adopted in the immobilization of other protein-based bioentities thus expanding its real-world utility. NRF (Natl Research Foundation, S’pore) 2019-07-10T04:10:05Z 2019-12-06T16:09:47Z 2019-07-10T04:10:05Z 2019-12-06T16:09:47Z 2018 Journal Article Ye, K., Sinawang, P. D., Tok, A. I. Y., & Marks, R. S. (2018). Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 256, 234-242. doi:10.1016/j.snb.2017.10.058 0925-4005 https://hdl.handle.net/10356/85760 http://hdl.handle.net/10220/49245 10.1016/j.snb.2017.10.058 en Sensors and Actuators B: Chemical © 2017 Elsevier B.V. All rights reserved.
spellingShingle Diazirine Silane
Photoinducible Crosslinker
Engineering::Materials
Ye, Kehan
Sinawang, Prima Dewi
Tok, Alfred Iing Yoong
Marks, Robert S.
Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title_full Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title_fullStr Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title_full_unstemmed Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title_short Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
title_sort photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model e coli analyte
topic Diazirine Silane
Photoinducible Crosslinker
Engineering::Materials
url https://hdl.handle.net/10356/85760
http://hdl.handle.net/10220/49245
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