Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte
One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemil...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2019
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| Online Access: | https://hdl.handle.net/10356/85760 http://hdl.handle.net/10220/49245 |
| _version_ | 1826125088997507072 |
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| author | Ye, Kehan Sinawang, Prima Dewi Tok, Alfred Iing Yoong Marks, Robert S. |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Ye, Kehan Sinawang, Prima Dewi Tok, Alfred Iing Yoong Marks, Robert S. |
| author_sort | Ye, Kehan |
| collection | NTU |
| description | One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. After optimization, it has shown superior adaptability over traditional chemiluminescent ELISA and a commonly used glutaraldehyde chemical immobilization method in detection limit (6.44 * 102 CFU/ml) and dynamic range (6.44 *105 to 6.44 *108 CFU/ml, r2 = 0.98; 6.44 *102 to 6.44 *105, r2 = 0.92). The newly developed immobilization method has enhanced the function of FOBS as an affordable and dispatchable tool in the early warning monitoring of the putative presence of E. coli cells. It can be used either in individual sample screening or in continuous monitoring of the environment to indicate the need for further investigation. The diazirine crosslinking method can also be adopted in the immobilization of other protein-based bioentities thus expanding its real-world utility. |
| first_indexed | 2024-10-01T06:31:03Z |
| format | Journal Article |
| id | ntu-10356/85760 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:31:03Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | ntu-10356/857602020-06-01T10:21:16Z Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte Ye, Kehan Sinawang, Prima Dewi Tok, Alfred Iing Yoong Marks, Robert S. School of Materials Science & Engineering Diazirine Silane Photoinducible Crosslinker Engineering::Materials One of the greatest challenges that define the performance of a fibre optic biosensor (FOBS) is the immobilization of the bio-receptor molecules onto the surface of fibre optic. In this study, a photoinducible silane diazirine was developed as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. After optimization, it has shown superior adaptability over traditional chemiluminescent ELISA and a commonly used glutaraldehyde chemical immobilization method in detection limit (6.44 * 102 CFU/ml) and dynamic range (6.44 *105 to 6.44 *108 CFU/ml, r2 = 0.98; 6.44 *102 to 6.44 *105, r2 = 0.92). The newly developed immobilization method has enhanced the function of FOBS as an affordable and dispatchable tool in the early warning monitoring of the putative presence of E. coli cells. It can be used either in individual sample screening or in continuous monitoring of the environment to indicate the need for further investigation. The diazirine crosslinking method can also be adopted in the immobilization of other protein-based bioentities thus expanding its real-world utility. NRF (Natl Research Foundation, S’pore) 2019-07-10T04:10:05Z 2019-12-06T16:09:47Z 2019-07-10T04:10:05Z 2019-12-06T16:09:47Z 2018 Journal Article Ye, K., Sinawang, P. D., Tok, A. I. Y., & Marks, R. S. (2018). Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte. Sensors and Actuators B: Chemical, 256, 234-242. doi:10.1016/j.snb.2017.10.058 0925-4005 https://hdl.handle.net/10356/85760 http://hdl.handle.net/10220/49245 10.1016/j.snb.2017.10.058 en Sensors and Actuators B: Chemical © 2017 Elsevier B.V. All rights reserved. |
| spellingShingle | Diazirine Silane Photoinducible Crosslinker Engineering::Materials Ye, Kehan Sinawang, Prima Dewi Tok, Alfred Iing Yoong Marks, Robert S. Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title | Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title_full | Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title_fullStr | Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title_full_unstemmed | Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title_short | Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte |
| title_sort | photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model e coli analyte |
| topic | Diazirine Silane Photoinducible Crosslinker Engineering::Materials |
| url | https://hdl.handle.net/10356/85760 http://hdl.handle.net/10220/49245 |
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