Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells
RNA interfering (RNAi) using short interfering RNA (siRNA) is becoming a promising approach for cancer gene therapy. However, owing to the lack of safe and efficient carriers, the application of RNAi for clinical use is still very limited. In this study, we have developed cadmium sulphoselenide/Zinc...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/86204 http://hdl.handle.net/10220/45381 |
| _version_ | 1824453808259858432 |
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| author | Lin, Guimiao Chen, Ting Zou, Jinyun Wang, Yucheng Wang, Xiaomei Li, Jiefeng Huang, Qijun Fu, Zicai Zhao, Yingying Lin, Marie Chia-Mi Xu, Gaixia Yong, Ken-Tye |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Lin, Guimiao Chen, Ting Zou, Jinyun Wang, Yucheng Wang, Xiaomei Li, Jiefeng Huang, Qijun Fu, Zicai Zhao, Yingying Lin, Marie Chia-Mi Xu, Gaixia Yong, Ken-Tye |
| author_sort | Lin, Guimiao |
| collection | NTU |
| description | RNA interfering (RNAi) using short interfering RNA (siRNA) is becoming a promising approach for cancer gene therapy. However, owing to the lack of safe and efficient carriers, the application of RNAi for clinical use is still very limited. In this study, we have developed cadmium sulphoselenide/Zinc sulfide quantum dots (CdSSe/ZnS QDs)-based nanocarriers for in vitro gene delivery. These CdSSe/ZnS QDs are functionalized with polyethyleneimine (PEI) to form stable nanoplex (QD-PEI) and subsequently they are used for siRNA loading which specially targets human telomerase reverse transcriptase (TERT). High gene transfection efficiency (>80%) was achieved on two glioblastoma cell lines, U87 and U251. The gene expression level (49.99 ± 10.23% for U87, 43.28 ± 9.66% for U251) and protein expression level (51.58 ± 7.88% for U87, 50.69 ± 7.59% for U251) of TERT is observed to decrease substantially after transfecting the tumor cells for 48 h. More importantly, the silencing of TERT gene expression significantly suppressed the proliferation of glioblastoma cells. No obvious cytotoxicity from these QD-PEI nanoplexes were observed over at 10 times of the transfected doses. Based on these results, we envision that QDs engineered here can be used as a safe and efficient gene nanocarrier for siRNA delivery and a promising tool for future cancer gene therapy applications. |
| first_indexed | 2025-02-19T03:12:18Z |
| format | Journal Article |
| id | ntu-10356/86204 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:12:18Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/862042020-03-07T13:57:26Z Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells Lin, Guimiao Chen, Ting Zou, Jinyun Wang, Yucheng Wang, Xiaomei Li, Jiefeng Huang, Qijun Fu, Zicai Zhao, Yingying Lin, Marie Chia-Mi Xu, Gaixia Yong, Ken-Tye School of Electrical and Electronic Engineering Quantum Dots RNA Interfering RNA interfering (RNAi) using short interfering RNA (siRNA) is becoming a promising approach for cancer gene therapy. However, owing to the lack of safe and efficient carriers, the application of RNAi for clinical use is still very limited. In this study, we have developed cadmium sulphoselenide/Zinc sulfide quantum dots (CdSSe/ZnS QDs)-based nanocarriers for in vitro gene delivery. These CdSSe/ZnS QDs are functionalized with polyethyleneimine (PEI) to form stable nanoplex (QD-PEI) and subsequently they are used for siRNA loading which specially targets human telomerase reverse transcriptase (TERT). High gene transfection efficiency (>80%) was achieved on two glioblastoma cell lines, U87 and U251. The gene expression level (49.99 ± 10.23% for U87, 43.28 ± 9.66% for U251) and protein expression level (51.58 ± 7.88% for U87, 50.69 ± 7.59% for U251) of TERT is observed to decrease substantially after transfecting the tumor cells for 48 h. More importantly, the silencing of TERT gene expression significantly suppressed the proliferation of glioblastoma cells. No obvious cytotoxicity from these QD-PEI nanoplexes were observed over at 10 times of the transfected doses. Based on these results, we envision that QDs engineered here can be used as a safe and efficient gene nanocarrier for siRNA delivery and a promising tool for future cancer gene therapy applications. ASTAR (Agency for Sci., Tech. and Research, S’pore) Published version 2018-07-30T06:53:01Z 2019-12-06T16:17:59Z 2018-07-30T06:53:01Z 2019-12-06T16:17:59Z 2017 Journal Article Lin, G., Chen, T., Zou, J., Wang, Y., Wang, X., Li, J., et al. (2017). Quantum Dots-siRNA Nanoplexes for Gene Silencing in Central Nervous System Tumor Cells. Frontiers in Pharmacology, 8, 182-. https://hdl.handle.net/10356/86204 http://hdl.handle.net/10220/45381 10.3389/fphar.2017.00182 en Frontiers in Pharmacology © 2017 Lin, Chen, Zou, Wang, Wang, Li, Huang, Fu, Zhao, Lin, Xu and Yong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. 10 p. application/pdf |
| spellingShingle | Quantum Dots RNA Interfering Lin, Guimiao Chen, Ting Zou, Jinyun Wang, Yucheng Wang, Xiaomei Li, Jiefeng Huang, Qijun Fu, Zicai Zhao, Yingying Lin, Marie Chia-Mi Xu, Gaixia Yong, Ken-Tye Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title | Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title_full | Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title_fullStr | Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title_full_unstemmed | Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title_short | Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells |
| title_sort | quantum dots sirna nanoplexes for gene silencing in central nervous system tumor cells |
| topic | Quantum Dots RNA Interfering |
| url | https://hdl.handle.net/10356/86204 http://hdl.handle.net/10220/45381 |
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