Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy
Aptamer-based biosensing, which uses short, single-stranded nucleic acid segments to bind to a target, can be advantageous over antibody-based diagnostics due to the ease of synthesis and high stability of aptamers. However, the development of most aptamer-based sensors (aptasensors) is still in its...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/86263 http://hdl.handle.net/10220/44007 |
| _version_ | 1811688183944970240 |
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| author | Geldert, Alisha Kenry Zhang, Xiao Zhang, Hua Lim, Chwee Teck |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Geldert, Alisha Kenry Zhang, Xiao Zhang, Hua Lim, Chwee Teck |
| author_sort | Geldert, Alisha |
| collection | NTU |
| description | Aptamer-based biosensing, which uses short, single-stranded nucleic acid segments to bind to a target, can be advantageous over antibody-based diagnostics due to the ease of synthesis and high stability of aptamers. However, the development of most aptamer-based sensors (aptasensors) is still in its initial stages and many factors affecting their performance have not been studied in great detail. Here, we enhance the sensing specificity of a fluorescence resonance energy transfer (FRET)-based MoS2 nanosheet aptasensor in detecting the malarial biomarker Plasmodium lactate dehydrogenase (pLDH). In this sensing scheme, the presence of target is signaled by an increase in fluorescence when fluorescently-labeled aptamers bind to pLDH and release from a quenching material. Interestingly, unlike most of the reported literature on aptasensors, we observe that non-target proteins also cause a considerable increase in the detected fluorescence. This may be due to the nonspecific adsorption of proteins onto the fluorescence quencher, leading to the displacement of aptamers from the quencher surface. To reduce this nonspecific association and to enhance the sensor specificity, we propose the application of a surface blocking agent to the quenching material. Importantly, we demonstrate that the sensing specificity of the MoS2 nanosheet-based aptasensor towards target pLDH biomolecules can be significantly enhanced through surface passivation, thus contributing to the development of highly selective and robust point-of-care malaria diagnostics. |
| first_indexed | 2024-10-01T05:28:10Z |
| format | Journal Article |
| id | ntu-10356/86263 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:28:10Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/862632021-01-08T07:08:24Z Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy Geldert, Alisha Kenry Zhang, Xiao Zhang, Hua Lim, Chwee Teck School of Materials Science & Engineering Interdisciplinary Graduate School (IGS) Energy Research Institute @ NTU (ERI@N) Biosensing Aptasensor Aptamer-based biosensing, which uses short, single-stranded nucleic acid segments to bind to a target, can be advantageous over antibody-based diagnostics due to the ease of synthesis and high stability of aptamers. However, the development of most aptamer-based sensors (aptasensors) is still in its initial stages and many factors affecting their performance have not been studied in great detail. Here, we enhance the sensing specificity of a fluorescence resonance energy transfer (FRET)-based MoS2 nanosheet aptasensor in detecting the malarial biomarker Plasmodium lactate dehydrogenase (pLDH). In this sensing scheme, the presence of target is signaled by an increase in fluorescence when fluorescently-labeled aptamers bind to pLDH and release from a quenching material. Interestingly, unlike most of the reported literature on aptasensors, we observe that non-target proteins also cause a considerable increase in the detected fluorescence. This may be due to the nonspecific adsorption of proteins onto the fluorescence quencher, leading to the displacement of aptamers from the quencher surface. To reduce this nonspecific association and to enhance the sensor specificity, we propose the application of a surface blocking agent to the quenching material. Importantly, we demonstrate that the sensing specificity of the MoS2 nanosheet-based aptasensor towards target pLDH biomolecules can be significantly enhanced through surface passivation, thus contributing to the development of highly selective and robust point-of-care malaria diagnostics. NRF (Natl Research Foundation, S’pore) MOE (Min. of Education, S’pore) Accepted version 2017-11-08T04:57:44Z 2019-12-06T16:19:12Z 2017-11-08T04:57:44Z 2019-12-06T16:19:12Z 2017 Journal Article Geldert, A., Kenry, Zhang, X., Zhang, H., & Lim, C. T. (2017). Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy. Analyst, 142(14), 2570-2577. 0003-2654 https://hdl.handle.net/10356/86263 http://hdl.handle.net/10220/44007 10.1039/C7AN00640C en Analyst © 2017 The Royal Society of Chemistry. This is the author created version of a work that has been peer reviewed and accepted for publication by Analyst, The Royal Society of Chemistry. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1039/C7AN00640C]. 16 p. application/pdf |
| spellingShingle | Biosensing Aptasensor Geldert, Alisha Kenry Zhang, Xiao Zhang, Hua Lim, Chwee Teck Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title | Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title_full | Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title_fullStr | Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title_full_unstemmed | Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title_short | Enhancing the sensing specificity of a MoS2 nanosheet-based FRET aptasensor using a surface blocking strategy |
| title_sort | enhancing the sensing specificity of a mos2 nanosheet based fret aptasensor using a surface blocking strategy |
| topic | Biosensing Aptasensor |
| url | https://hdl.handle.net/10356/86263 http://hdl.handle.net/10220/44007 |
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