Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex
TMEM132D is a candidate gene, where risk genotypes have been associated with anxiety severity along with higher mRNA expression in the frontal cortex of panic disorder patients. Concurrently, in a high (HAB) and low (LAB) trait anxiety mouse model, Tmem132d was found to show increased expression in...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/86287 http://hdl.handle.net/10220/45290 |
| _version_ | 1811681468523479040 |
|---|---|
| author | Naik, Roshan R. Sotnikov, Sergey V. Diepold, Rebekka P. Iurato, Stella Markt, Patrick O. Bultmann, Andrea Brehm, Nadine Mattheus, Tobias Lutz, Beat Erhardt, Angelika Binder, Elisabeth B. Schmidt, Ulrike Holsboer, Florian Landgraf, Rainer Czibere, Ludwig |
| author2 | Lee Kong Chian School of Medicine (LKCMedicine) |
| author_facet | Lee Kong Chian School of Medicine (LKCMedicine) Naik, Roshan R. Sotnikov, Sergey V. Diepold, Rebekka P. Iurato, Stella Markt, Patrick O. Bultmann, Andrea Brehm, Nadine Mattheus, Tobias Lutz, Beat Erhardt, Angelika Binder, Elisabeth B. Schmidt, Ulrike Holsboer, Florian Landgraf, Rainer Czibere, Ludwig |
| author_sort | Naik, Roshan R. |
| collection | NTU |
| description | TMEM132D is a candidate gene, where risk genotypes have been associated with anxiety severity along with higher mRNA expression in the frontal cortex of panic disorder patients. Concurrently, in a high (HAB) and low (LAB) trait anxiety mouse model, Tmem132d was found to show increased expression in the anterior cingulate cortex (aCC) of HAB as compared to LAB mice. To understand the molecular underpinnings underlying the differential expression, we sequenced the gene and found two single-nucleotide polymorphisms (SNPs) in the promoter differing between both lines which could explain the observed mRNA expression profiles using gene reporter assays. In addition, there was no difference in basal DNA methylation in the CpG Island that encompasses the HAB vs. LAB Tmem132d promoter region. Furthermore, we found significantly higher binding of RNA polymerase II (POLR2A) to the proximal HAB-specific SNP (rs233264624) than the corresponding LAB locus in an oligonucleotide pull-down assay, suggesting increased transcription. Virus mediated overexpression of Tmem132d in the aCC of C57BL/6 J mice could confirm its role in mediating an anxiogenic phenotype. To model gene–environmental interactions, HAB mice exposed to enriched environment (HAB-EE) responded with decreased anxiety levels but, had enhanced Tmem132d mRNA expression as compared to standard-housed HAB (HAB-SH) mice. While LAB mice subjected to unpredictable chronic mild stress (LAB-UCMS) exhibited higher anxiety levels and had lower mRNA expression compared to standard-housed LAB (LAB-SH) mice. Chromatin immunoprecipitation revealed significantly higher binding of POLR2A to rs233264624 in HAB-EE, while LAB-UCMS had lower POLR2A binding at this locus, thus explaining the enhanced or attenuated expression of Tmem132d compared to their respective SH controls. To further investigate gene–environment interactions, DNA methylation was assessed using Illumina 450 K BeadChip in 74 panic disorder patients. Significant methylation differences were observed in two CpGs (cg26322591 and cg03283235) located in TMEM132D depending on the number of positive life events supporting the results of an influence of positive environmental cues on regulation of Tmem132d expression in mice. |
| first_indexed | 2024-10-01T03:41:26Z |
| format | Journal Article |
| id | ntu-10356/86287 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:41:26Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/862872020-11-01T05:15:10Z Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex Naik, Roshan R. Sotnikov, Sergey V. Diepold, Rebekka P. Iurato, Stella Markt, Patrick O. Bultmann, Andrea Brehm, Nadine Mattheus, Tobias Lutz, Beat Erhardt, Angelika Binder, Elisabeth B. Schmidt, Ulrike Holsboer, Florian Landgraf, Rainer Czibere, Ludwig Lee Kong Chian School of Medicine (LKCMedicine) Clinical Genetics Genomics TMEM132D is a candidate gene, where risk genotypes have been associated with anxiety severity along with higher mRNA expression in the frontal cortex of panic disorder patients. Concurrently, in a high (HAB) and low (LAB) trait anxiety mouse model, Tmem132d was found to show increased expression in the anterior cingulate cortex (aCC) of HAB as compared to LAB mice. To understand the molecular underpinnings underlying the differential expression, we sequenced the gene and found two single-nucleotide polymorphisms (SNPs) in the promoter differing between both lines which could explain the observed mRNA expression profiles using gene reporter assays. In addition, there was no difference in basal DNA methylation in the CpG Island that encompasses the HAB vs. LAB Tmem132d promoter region. Furthermore, we found significantly higher binding of RNA polymerase II (POLR2A) to the proximal HAB-specific SNP (rs233264624) than the corresponding LAB locus in an oligonucleotide pull-down assay, suggesting increased transcription. Virus mediated overexpression of Tmem132d in the aCC of C57BL/6 J mice could confirm its role in mediating an anxiogenic phenotype. To model gene–environmental interactions, HAB mice exposed to enriched environment (HAB-EE) responded with decreased anxiety levels but, had enhanced Tmem132d mRNA expression as compared to standard-housed HAB (HAB-SH) mice. While LAB mice subjected to unpredictable chronic mild stress (LAB-UCMS) exhibited higher anxiety levels and had lower mRNA expression compared to standard-housed LAB (LAB-SH) mice. Chromatin immunoprecipitation revealed significantly higher binding of POLR2A to rs233264624 in HAB-EE, while LAB-UCMS had lower POLR2A binding at this locus, thus explaining the enhanced or attenuated expression of Tmem132d compared to their respective SH controls. To further investigate gene–environment interactions, DNA methylation was assessed using Illumina 450 K BeadChip in 74 panic disorder patients. Significant methylation differences were observed in two CpGs (cg26322591 and cg03283235) located in TMEM132D depending on the number of positive life events supporting the results of an influence of positive environmental cues on regulation of Tmem132d expression in mice. Published version 2018-07-27T03:23:58Z 2019-12-06T16:19:40Z 2018-07-27T03:23:58Z 2019-12-06T16:19:40Z 2017 Journal Article Naik, R. R., Sotnikov, S. V., Diepold, R. P., Iurato, S., Markt, P. O., Bultmann, A., et al. (2017). Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex. Translational Psychiatry, 8(1), 25-. https://hdl.handle.net/10356/86287 http://hdl.handle.net/10220/45290 10.1038/s41398-017-0025-2 en Translational Psychiatry © 2017 The Author(s) (Nature Publishing Group). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. 18 p. application/pdf |
| spellingShingle | Clinical Genetics Genomics Naik, Roshan R. Sotnikov, Sergey V. Diepold, Rebekka P. Iurato, Stella Markt, Patrick O. Bultmann, Andrea Brehm, Nadine Mattheus, Tobias Lutz, Beat Erhardt, Angelika Binder, Elisabeth B. Schmidt, Ulrike Holsboer, Florian Landgraf, Rainer Czibere, Ludwig Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title | Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title_full | Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title_fullStr | Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title_full_unstemmed | Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title_short | Polymorphism in Tmem132d regulates expression and anxiety-related behavior through binding of RNA polymerase II complex |
| title_sort | polymorphism in tmem132d regulates expression and anxiety related behavior through binding of rna polymerase ii complex |
| topic | Clinical Genetics Genomics |
| url | https://hdl.handle.net/10356/86287 http://hdl.handle.net/10220/45290 |
| work_keys_str_mv | AT naikroshanr polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT sotnikovsergeyv polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT diepoldrebekkap polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT iuratostella polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT marktpatricko polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT bultmannandrea polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT brehmnadine polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT mattheustobias polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT lutzbeat polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT erhardtangelika polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT binderelisabethb polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT schmidtulrike polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT holsboerflorian polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT landgrafrainer polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex AT czibereludwig polymorphismintmem132dregulatesexpressionandanxietyrelatedbehaviorthroughbindingofrnapolymeraseiicomplex |