Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo
Host defence peptides (HDPs) derived from the C-terminus of thrombin are proteolytically generated by enzymes released during inflammation and wounding. In this work, we studied the effects of the prototypic peptide GKY25 (GKYGFYTHVFRLKKWIQKVIDQFGE), on neutrophil functions. In vitro, GKY25 was show...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/86509 http://hdl.handle.net/10220/44087 |
| _version_ | 1811697157630066688 |
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| author | Lim, Chun Hwee Puthia, Manoj Butrym, Marta Tay, Hui Min Lee, Michelle Zi Yi Hou, Han Wei Schmidtchen, Artur |
| author2 | Interdisciplinary Graduate School (IGS) |
| author_facet | Interdisciplinary Graduate School (IGS) Lim, Chun Hwee Puthia, Manoj Butrym, Marta Tay, Hui Min Lee, Michelle Zi Yi Hou, Han Wei Schmidtchen, Artur |
| author_sort | Lim, Chun Hwee |
| collection | NTU |
| description | Host defence peptides (HDPs) derived from the C-terminus of thrombin are proteolytically generated by enzymes released during inflammation and wounding. In this work, we studied the effects of the prototypic peptide GKY25 (GKYGFYTHVFRLKKWIQKVIDQFGE), on neutrophil functions. In vitro, GKY25 was shown to decrease LPS-induced neutrophil activation. In addition, the peptide induced CD62L shedding on neutrophils without inducing their activation. Correspondingly, GKY25-treated neutrophils showed reduced attachment and rolling behaviour on surfaces coated with the CD62L ligand E-selectin. The GKY25-treated neutrophils also displayed a dampened chemotactic response against the chemokine IL-8. Furthermore, in vivo, mice treated with GKY25 exhibited a reduced local ROS response against LPS. Taken together, our results show that GKY25 can modulate neutrophil functions in vitro and in vivo. |
| first_indexed | 2024-10-01T07:50:48Z |
| format | Journal Article |
| id | ntu-10356/86509 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:50:48Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/865092020-11-01T04:45:40Z Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo Lim, Chun Hwee Puthia, Manoj Butrym, Marta Tay, Hui Min Lee, Michelle Zi Yi Hou, Han Wei Schmidtchen, Artur Interdisciplinary Graduate School (IGS) Lee Kong Chian School of Medicine (LKCMedicine) Neutrophils Sepsis Host defence peptides (HDPs) derived from the C-terminus of thrombin are proteolytically generated by enzymes released during inflammation and wounding. In this work, we studied the effects of the prototypic peptide GKY25 (GKYGFYTHVFRLKKWIQKVIDQFGE), on neutrophil functions. In vitro, GKY25 was shown to decrease LPS-induced neutrophil activation. In addition, the peptide induced CD62L shedding on neutrophils without inducing their activation. Correspondingly, GKY25-treated neutrophils showed reduced attachment and rolling behaviour on surfaces coated with the CD62L ligand E-selectin. The GKY25-treated neutrophils also displayed a dampened chemotactic response against the chemokine IL-8. Furthermore, in vivo, mice treated with GKY25 exhibited a reduced local ROS response against LPS. Taken together, our results show that GKY25 can modulate neutrophil functions in vitro and in vivo. MOE (Min. of Education, S’pore) Published version 2017-11-24T08:44:05Z 2019-12-06T16:23:38Z 2017-11-24T08:44:05Z 2019-12-06T16:23:38Z 2017 Journal Article Lim, C. H., Puthia, M., Butrym, M., Tay, H. M., Lee, M. Z. Y., Hou, H. W., et al. (2017). Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo. Scientific Reports, 7, 11201-. https://hdl.handle.net/10356/86509 http://hdl.handle.net/10220/44087 10.1038/s41598-017-11464-x en Scientific Reports © 2017 The Author(s). Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. 12 p. application/pdf |
| spellingShingle | Neutrophils Sepsis Lim, Chun Hwee Puthia, Manoj Butrym, Marta Tay, Hui Min Lee, Michelle Zi Yi Hou, Han Wei Schmidtchen, Artur Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title | Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title_full | Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title_fullStr | Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title_full_unstemmed | Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title_short | Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| title_sort | thrombin derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo |
| topic | Neutrophils Sepsis |
| url | https://hdl.handle.net/10356/86509 http://hdl.handle.net/10220/44087 |
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