Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation
Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiat...
| Main Authors: | , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/86534 http://hdl.handle.net/10220/44040 |
| _version_ | 1811691290198278144 |
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| author | Edupuganti, Raghu Ram Harikumar, Arigela Aaronson, Yair Biran, Alva Sailaja, Badi Sri Nissim-Rafinia, Malka Azad, Gajendra Kumar Cohen, Malkiel A. Park, Jung Eun Shivalila, Chikdu S. Markoulaki, Styliani Sze, Siu Kwan Jaenisch, Rudolf Meshorer, Eran |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Edupuganti, Raghu Ram Harikumar, Arigela Aaronson, Yair Biran, Alva Sailaja, Badi Sri Nissim-Rafinia, Malka Azad, Gajendra Kumar Cohen, Malkiel A. Park, Jung Eun Shivalila, Chikdu S. Markoulaki, Styliani Sze, Siu Kwan Jaenisch, Rudolf Meshorer, Eran |
| author_sort | Edupuganti, Raghu Ram |
| collection | NTU |
| description | Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage. |
| first_indexed | 2024-10-01T06:17:32Z |
| format | Journal Article |
| id | ntu-10356/86534 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:17:32Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/865342023-02-28T17:01:26Z Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation Edupuganti, Raghu Ram Harikumar, Arigela Aaronson, Yair Biran, Alva Sailaja, Badi Sri Nissim-Rafinia, Malka Azad, Gajendra Kumar Cohen, Malkiel A. Park, Jung Eun Shivalila, Chikdu S. Markoulaki, Styliani Sze, Siu Kwan Jaenisch, Rudolf Meshorer, Eran School of Biological Sciences SET Embryonic Stem Cells Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage. Published version 2017-11-15T03:34:29Z 2019-12-06T16:24:10Z 2017-11-15T03:34:29Z 2019-12-06T16:24:10Z 2017 Journal Article Edupuganti, R. R., Harikumar, A., Aaronson, Y., Biran, A., Sailaja, B. S., Nissim-Rafinia, M., et al. (2017). Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. Stem Cell Reports, 9(4), 1291-1303. 2213-6711 https://hdl.handle.net/10356/86534 http://hdl.handle.net/10220/44040 10.1016/j.stemcr.2017.08.021 en Stem Cell Reports © 2017 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 13 p. application/pdf |
| spellingShingle | SET Embryonic Stem Cells Edupuganti, Raghu Ram Harikumar, Arigela Aaronson, Yair Biran, Alva Sailaja, Badi Sri Nissim-Rafinia, Malka Azad, Gajendra Kumar Cohen, Malkiel A. Park, Jung Eun Shivalila, Chikdu S. Markoulaki, Styliani Sze, Siu Kwan Jaenisch, Rudolf Meshorer, Eran Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title | Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title_full | Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title_fullStr | Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title_full_unstemmed | Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title_short | Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation |
| title_sort | alternative set tafi promoters regulate embryonic stem cell differentiation |
| topic | SET Embryonic Stem Cells |
| url | https://hdl.handle.net/10356/86534 http://hdl.handle.net/10220/44040 |
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