The central element of the synaptonemal complex in mice is organized as a bilayered junction structure
The synaptonemal complex transiently stabilizes pairing interactions between homologous chromosomes during meiosis. Assembly of the synaptonemal complex is mediated through integration of opposing transverse filaments into a central element, a process that is poorly understood. We have, here, analyz...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2017
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| Online Access: | https://hdl.handle.net/10356/86678 http://hdl.handle.net/10220/44123 |
| _version_ | 1824453072843177984 |
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| author | Hernández-Hernández, Abrahan Masich, Sergej Fukuda, Tomoyuki Kouznetsova, Anna Sandin, Sara Daneholt, Bertil Höög, Christer |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Hernández-Hernández, Abrahan Masich, Sergej Fukuda, Tomoyuki Kouznetsova, Anna Sandin, Sara Daneholt, Bertil Höög, Christer |
| author_sort | Hernández-Hernández, Abrahan |
| collection | NTU |
| description | The synaptonemal complex transiently stabilizes pairing interactions between homologous chromosomes during meiosis. Assembly of the synaptonemal complex is mediated through integration of opposing transverse filaments into a central element, a process that is poorly understood. We have, here, analyzed the localization of the transverse filament protein SYCP1 and the central element proteins SYCE1, SYCE2 and SYCE3 within the central region of the synaptonemal complex in mouse spermatocytes using immunoelectron microscopy. Distribution of immuno-gold particles in a lateral view of the synaptonemal complex, supported by protein interaction data, suggest that the N-terminal region of SYCP1 and SYCE3 form a joint bilayered central structure, and that SYCE1 and SYCE2 localize in between the two layers. We find that disruption of SYCE2 and TEX12 (a fourth central element protein) localization to the central element abolishes central alignment of the N-terminal region of SYCP1. Thus, our results show that all four central element proteins, in an interdependent manner, contribute to stabilization of opposing N-terminal regions of SYCP1, forming a bilayered transverse-filament–central-element junction structure that promotes synaptonemal complex formation and synapsis. |
| first_indexed | 2024-10-01T02:59:02Z |
| format | Journal Article |
| id | ntu-10356/86678 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:59:02Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/866782023-02-28T17:01:42Z The central element of the synaptonemal complex in mice is organized as a bilayered junction structure Hernández-Hernández, Abrahan Masich, Sergej Fukuda, Tomoyuki Kouznetsova, Anna Sandin, Sara Daneholt, Bertil Höög, Christer School of Biological Sciences Synaptonemal Complex Meiosis The synaptonemal complex transiently stabilizes pairing interactions between homologous chromosomes during meiosis. Assembly of the synaptonemal complex is mediated through integration of opposing transverse filaments into a central element, a process that is poorly understood. We have, here, analyzed the localization of the transverse filament protein SYCP1 and the central element proteins SYCE1, SYCE2 and SYCE3 within the central region of the synaptonemal complex in mouse spermatocytes using immunoelectron microscopy. Distribution of immuno-gold particles in a lateral view of the synaptonemal complex, supported by protein interaction data, suggest that the N-terminal region of SYCP1 and SYCE3 form a joint bilayered central structure, and that SYCE1 and SYCE2 localize in between the two layers. We find that disruption of SYCE2 and TEX12 (a fourth central element protein) localization to the central element abolishes central alignment of the N-terminal region of SYCP1. Thus, our results show that all four central element proteins, in an interdependent manner, contribute to stabilization of opposing N-terminal regions of SYCP1, forming a bilayered transverse-filament–central-element junction structure that promotes synaptonemal complex formation and synapsis. MOE (Min. of Education, S’pore) Published version 2017-12-12T05:41:19Z 2019-12-06T16:27:09Z 2017-12-12T05:41:19Z 2019-12-06T16:27:09Z 2016 Journal Article Hernández-Hernández, A., Masich, S., Fukuda, T., Kouznetsova, A., Sandin, S., Daneholt, B., et al. (2016). The central element of the synaptonemal complex in mice is organized as a bilayered junction structure. Journal of Cell Science, 129(11), 2239-2249. 0021-9533 https://hdl.handle.net/10356/86678 http://hdl.handle.net/10220/44123 10.1242/jcs.182477 en Journal of Cell Science © 2016 The Author(s) (published by The Company of Biologists Ltd). This paper was published in Journal of Cell Science and is made available as an electronic reprint (preprint) with permission of The Author(s) (published by The Company of Biologists Ltd). The published version is available at: [http://dx.doi.org/10.1242/jcs.182477]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 11 p. application/pdf |
| spellingShingle | Synaptonemal Complex Meiosis Hernández-Hernández, Abrahan Masich, Sergej Fukuda, Tomoyuki Kouznetsova, Anna Sandin, Sara Daneholt, Bertil Höög, Christer The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title | The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title_full | The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title_fullStr | The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title_full_unstemmed | The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title_short | The central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| title_sort | central element of the synaptonemal complex in mice is organized as a bilayered junction structure |
| topic | Synaptonemal Complex Meiosis |
| url | https://hdl.handle.net/10356/86678 http://hdl.handle.net/10220/44123 |
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