The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation
Starvation triggers global alterations in the synthesis and turnover of proteins. Under such conditions, the recycling of essential nutrients by using autophagy is indispensable for survival. By screening known kinases in the yeast genome, we newly identified a regulator of autophagy, the Ccl1–Kin28...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2017
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| Online Access: | https://hdl.handle.net/10356/86679 http://hdl.handle.net/10220/44122 |
| _version_ | 1826118221839728640 |
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| author | Zhu, Jing Deng, Shuangsheng Lu, Puzhong Bu, Wenting Li, Tian Yu, Li Xie, Zhiping |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Zhu, Jing Deng, Shuangsheng Lu, Puzhong Bu, Wenting Li, Tian Yu, Li Xie, Zhiping |
| author_sort | Zhu, Jing |
| collection | NTU |
| description | Starvation triggers global alterations in the synthesis and turnover of proteins. Under such conditions, the recycling of essential nutrients by using autophagy is indispensable for survival. By screening known kinases in the yeast genome, we newly identified a regulator of autophagy, the Ccl1–Kin28 kinase complex (the equivalent of the mammalian cyclin-H–Cdk7 complex), which is known to play key roles in RNA-polymerase-II-mediated transcription. We show that inactivation of Ccl1 caused complete block of autophagy. Interestingly, Ccl1 itself was subject to proteasomal degradation, limiting the level of autophagy during prolonged starvation. We present further evidence that the Ccl1–Kin28 complex regulates the expression of Atg29 and Atg31, which is crucial in the assembly of the Atg1 kinase complex. The identification of this previously unknown regulatory pathway sheds new light on the complex signaling network that governs autophagy activity. |
| first_indexed | 2024-10-01T04:40:09Z |
| format | Journal Article |
| id | ntu-10356/86679 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:40:09Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | ntu-10356/866792023-02-28T17:01:43Z The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation Zhu, Jing Deng, Shuangsheng Lu, Puzhong Bu, Wenting Li, Tian Yu, Li Xie, Zhiping School of Biological Sciences Autophagy Kinase Starvation triggers global alterations in the synthesis and turnover of proteins. Under such conditions, the recycling of essential nutrients by using autophagy is indispensable for survival. By screening known kinases in the yeast genome, we newly identified a regulator of autophagy, the Ccl1–Kin28 kinase complex (the equivalent of the mammalian cyclin-H–Cdk7 complex), which is known to play key roles in RNA-polymerase-II-mediated transcription. We show that inactivation of Ccl1 caused complete block of autophagy. Interestingly, Ccl1 itself was subject to proteasomal degradation, limiting the level of autophagy during prolonged starvation. We present further evidence that the Ccl1–Kin28 complex regulates the expression of Atg29 and Atg31, which is crucial in the assembly of the Atg1 kinase complex. The identification of this previously unknown regulatory pathway sheds new light on the complex signaling network that governs autophagy activity. Published version 2017-12-12T05:41:06Z 2019-12-06T16:27:10Z 2017-12-12T05:41:06Z 2019-12-06T16:27:10Z 2016 Journal Article Zhu, J., Deng, S., Lu, P., Bu, W., Li, T., Yu, L., et al. (2016). The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation. Journal of Cell Science, 129(1), 135-144. 0021-9533 https://hdl.handle.net/10356/86679 http://hdl.handle.net/10220/44122 10.1242/jcs.177071 en Journal of Cell Science © 2016 The Author(s) (published by The Company of Biologists Ltd). This paper was published in Journal of Cell Science and is made available as an electronic reprint (preprint) with permission of The Author(s) (published by The Company of Biologists Ltd). The published version is available at: [http://dx.doi.org/10.1242/jcs.177071]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. 10 p. application/pdf |
| spellingShingle | Autophagy Kinase Zhu, Jing Deng, Shuangsheng Lu, Puzhong Bu, Wenting Li, Tian Yu, Li Xie, Zhiping The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title | The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title_full | The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title_fullStr | The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title_full_unstemmed | The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title_short | The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation |
| title_sort | ccl1 kin28 kinase complex regulates autophagy under nitrogen starvation |
| topic | Autophagy Kinase |
| url | https://hdl.handle.net/10356/86679 http://hdl.handle.net/10220/44122 |
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