Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid
Early detection of skin diseases is imperative for their effective treatment. However, fluorescence molecular probes that allow this are rare. The first activatable near-infrared (NIR) fluorescent molecular probe is reported for sensitive imaging of keloid cells, skin cells from abnormal scar fibrou...
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/87047 http://hdl.handle.net/10220/44322 |
| _version_ | 1811697789829120000 |
|---|---|
| author | Miao, Qingqing Yeo, David Chenloong Wiraja, Christian Zhang, Jianjian Ning, Xiaoyu Xu, Chenjie Pu, Kanyi |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Miao, Qingqing Yeo, David Chenloong Wiraja, Christian Zhang, Jianjian Ning, Xiaoyu Xu, Chenjie Pu, Kanyi |
| author_sort | Miao, Qingqing |
| collection | NTU |
| description | Early detection of skin diseases is imperative for their effective treatment. However, fluorescence molecular probes that allow this are rare. The first activatable near-infrared (NIR) fluorescent molecular probe is reported for sensitive imaging of keloid cells, skin cells from abnormal scar fibrous lesions. As keloid cells have high expression levels of fibroblast activation protein-alpha (FAPα), the probe (FNP1) is designed to have a caged NIR dye and a FAPα-cleavable peptide substrate linked by a self-immolative segment. FNP1 can quickly and specifically turn on its fluorescence at 710 nm by 45-fold in the presence of FAPα, allowing it to effectively recognize keloid cells from normal skin cells. Integration of FNP1 with a simple microneedle-assisted topical application enables sensitive detection of keloid cells in metabolically-active human skin tissue with a theoretical limit of detection down to 20 000 cells. |
| first_indexed | 2024-10-01T08:00:51Z |
| format | Journal Article |
| id | ntu-10356/87047 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T08:00:51Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/870472023-12-29T06:53:09Z Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid Miao, Qingqing Yeo, David Chenloong Wiraja, Christian Zhang, Jianjian Ning, Xiaoyu Xu, Chenjie Pu, Kanyi School of Chemical and Biomedical Engineering Fibroblast Activation Protein-alpha Molecular Probe DRNTU::Engineering::Chemical engineering Early detection of skin diseases is imperative for their effective treatment. However, fluorescence molecular probes that allow this are rare. The first activatable near-infrared (NIR) fluorescent molecular probe is reported for sensitive imaging of keloid cells, skin cells from abnormal scar fibrous lesions. As keloid cells have high expression levels of fibroblast activation protein-alpha (FAPα), the probe (FNP1) is designed to have a caged NIR dye and a FAPα-cleavable peptide substrate linked by a self-immolative segment. FNP1 can quickly and specifically turn on its fluorescence at 710 nm by 45-fold in the presence of FAPα, allowing it to effectively recognize keloid cells from normal skin cells. Integration of FNP1 with a simple microneedle-assisted topical application enables sensitive detection of keloid cells in metabolically-active human skin tissue with a theoretical limit of detection down to 20 000 cells. MOE (Min. of Education, S’pore) Accepted version 2018-01-18T03:14:53Z 2019-12-06T16:33:58Z 2018-01-18T03:14:53Z 2019-12-06T16:33:58Z 2018 2018 Journal Article Miao, Q., Yeo, D. C., Wiraja, C., Zhang, J., Ning, X., Xu, C., et al. (2018). Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid. Angewandte Chemie International Edition, in press. 1433-7851 https://hdl.handle.net/10356/87047 http://hdl.handle.net/10220/44322 10.1002/anie.201710727 203210 en Angewandte Chemie International Edition © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Angewandte Chemie International Edition, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1002/anie.201710727]. 6 p. application/pdf |
| spellingShingle | Fibroblast Activation Protein-alpha Molecular Probe DRNTU::Engineering::Chemical engineering Miao, Qingqing Yeo, David Chenloong Wiraja, Christian Zhang, Jianjian Ning, Xiaoyu Xu, Chenjie Pu, Kanyi Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title | Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title_full | Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title_fullStr | Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title_full_unstemmed | Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title_short | Near-Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid |
| title_sort | near infrared fluorescent molecular probe for sensitive imaging of keloid |
| topic | Fibroblast Activation Protein-alpha Molecular Probe DRNTU::Engineering::Chemical engineering |
| url | https://hdl.handle.net/10356/87047 http://hdl.handle.net/10220/44322 |
| work_keys_str_mv | AT miaoqingqing nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT yeodavidchenloong nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT wirajachristian nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT zhangjianjian nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT ningxiaoyu nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT xuchenjie nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid AT pukanyi nearinfraredfluorescentmolecularprobeforsensitiveimagingofkeloid |