DNA accumulation on ventilation system filters in university buildings in Singapore
Introduction: Biological particles deposit on air handling system filters as they process air. This study reports and interprets abundance and diversity information regarding biomass accumulation on ordinarily used filters acquired from several locations in a university environment. Methods: DNA-bas...
| প্রধান লেখক: | , , , , , |
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| অন্যান্য লেখক: | |
| বিন্যাস: | Journal Article |
| ভাষা: | English |
| প্রকাশিত: |
2018
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| অনলাইন ব্যবহার করুন: | https://hdl.handle.net/10356/87077 http://hdl.handle.net/10220/44281 |
| _version_ | 1826109454265876480 |
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| author | Luhung, Irvan Wu, Yan Xu, Siyu Yamamoto, Naomichi Chang, Victor Wei-Chung Nazaroff, William W. |
| author2 | Larcombe, Alexander |
| author_facet | Larcombe, Alexander Luhung, Irvan Wu, Yan Xu, Siyu Yamamoto, Naomichi Chang, Victor Wei-Chung Nazaroff, William W. |
| author_sort | Luhung, Irvan |
| collection | NTU |
| description | Introduction: Biological particles deposit on air handling system filters as they process air. This study reports and interprets abundance and diversity information regarding biomass accumulation on ordinarily used filters acquired from several locations in a university environment. Methods: DNA-based analysis was applied both to quantify (via DNA fluorometry and qPCR) and to characterize (via high-throughput sequencing) the microbial material on filters, which mainly processed recirculated indoor air. Results were interpreted in relation to building occupancy and ventilation system operational parameters. Results: Based on accumulated biomass, average DNA concentrations per AHU filter surface area across nine indoor locations after twelve weeks of filter use were in the respective ranges 1.1 to 41 ng per cm2 for total DNA, 0.02 to 3.3 ng per cm2 for bacterial DNA and 0.2 to 2.0 ng DNA per cm2 for fungal DNA. The most abundant genera detected on the AHU filter samples were Clostridium, Streptophyta, Bacillus, Acinetobacter and Ktedonobacter for bacteria and Aspergillus, Cladosporium, Nigrospora, Rigidoporus and Lentinus for fungi. Conditional indoor airborne DNA concentrations (median (range)) were estimated to be 13 (2.6–107) pg/m3 for total DNA, 0.4 (0.05–8.4) pg/m3 for bacterial DNA and 2.3 (1.0–5.1) pg/m3 for fungal DNA. Conclusion: Conditional airborne concentrations and the relative abundances of selected groups of genera correlate well with occupancy level. Bacterial DNA was found to be more responsive than fungal DNA to differences in occupancy level and indoor environmental conditions. |
| first_indexed | 2024-10-01T02:18:29Z |
| format | Journal Article |
| id | ntu-10356/87077 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:18:29Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/870772020-03-07T11:43:29Z DNA accumulation on ventilation system filters in university buildings in Singapore Luhung, Irvan Wu, Yan Xu, Siyu Yamamoto, Naomichi Chang, Victor Wei-Chung Nazaroff, William W. Larcombe, Alexander School of Civil and Environmental Engineering Introduction: Biological particles deposit on air handling system filters as they process air. This study reports and interprets abundance and diversity information regarding biomass accumulation on ordinarily used filters acquired from several locations in a university environment. Methods: DNA-based analysis was applied both to quantify (via DNA fluorometry and qPCR) and to characterize (via high-throughput sequencing) the microbial material on filters, which mainly processed recirculated indoor air. Results were interpreted in relation to building occupancy and ventilation system operational parameters. Results: Based on accumulated biomass, average DNA concentrations per AHU filter surface area across nine indoor locations after twelve weeks of filter use were in the respective ranges 1.1 to 41 ng per cm2 for total DNA, 0.02 to 3.3 ng per cm2 for bacterial DNA and 0.2 to 2.0 ng DNA per cm2 for fungal DNA. The most abundant genera detected on the AHU filter samples were Clostridium, Streptophyta, Bacillus, Acinetobacter and Ktedonobacter for bacteria and Aspergillus, Cladosporium, Nigrospora, Rigidoporus and Lentinus for fungi. Conditional indoor airborne DNA concentrations (median (range)) were estimated to be 13 (2.6–107) pg/m3 for total DNA, 0.4 (0.05–8.4) pg/m3 for bacterial DNA and 2.3 (1.0–5.1) pg/m3 for fungal DNA. Conclusion: Conditional airborne concentrations and the relative abundances of selected groups of genera correlate well with occupancy level. Bacterial DNA was found to be more responsive than fungal DNA to differences in occupancy level and indoor environmental conditions. Published version 2018-01-09T05:22:21Z 2019-12-06T16:34:39Z 2018-01-09T05:22:21Z 2019-12-06T16:34:39Z 2017 Journal Article Luhung, I., Wu, Y., Xu, S., Yamamoto, N., Chang, V. W.-C., & Nazaroff, W. W. (2017). DNA accumulation on ventilation system filters in university buildings in Singapore. PLOS ONE, 12(10), e0186295-. https://hdl.handle.net/10356/87077 http://hdl.handle.net/10220/44281 10.1371/journal.pone.0186295 en PLOS ONE © 2017 The Author(s) (Public Library of Science). This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. 18 p. application/pdf |
| spellingShingle | Luhung, Irvan Wu, Yan Xu, Siyu Yamamoto, Naomichi Chang, Victor Wei-Chung Nazaroff, William W. DNA accumulation on ventilation system filters in university buildings in Singapore |
| title | DNA accumulation on ventilation system filters in university buildings in Singapore |
| title_full | DNA accumulation on ventilation system filters in university buildings in Singapore |
| title_fullStr | DNA accumulation on ventilation system filters in university buildings in Singapore |
| title_full_unstemmed | DNA accumulation on ventilation system filters in university buildings in Singapore |
| title_short | DNA accumulation on ventilation system filters in university buildings in Singapore |
| title_sort | dna accumulation on ventilation system filters in university buildings in singapore |
| url | https://hdl.handle.net/10356/87077 http://hdl.handle.net/10220/44281 |
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