Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals
In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal’s muscular activity. He...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2018
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| Online Access: | https://hdl.handle.net/10356/87221 http://hdl.handle.net/10220/44381 |
| _version_ | 1826125413069357056 |
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| author | Miyagawa, Takuya Fujie, Toshinori Ferdinandus Vo Doan, Tat Thang Sato, Hirotaka Takeoka, Shinji |
| author2 | School of Mechanical and Aerospace Engineering |
| author_facet | School of Mechanical and Aerospace Engineering Miyagawa, Takuya Fujie, Toshinori Ferdinandus Vo Doan, Tat Thang Sato, Hirotaka Takeoka, Shinji |
| author_sort | Miyagawa, Takuya |
| collection | NTU |
| description | In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal’s muscular activity. Herein, we report super flexible and self-adhesive (no need of glue) nanothermosensor consisting of stacked two different polymeric nanosheets with thermosensitive (Eu-tris (dinaphthoylmethane)-bis-trioctylphosphine oxide: EuDT) and insensitive (Rhodamine 800) dyes being embedded. Such stacked nanosheets allow for the ratiometric thermometry, with which the undesired luminescence intensity shift due to focal drift or animal’s z-axis displacement is eliminated and the desired intensity shift solely due to the temperature shift of the sample (living muscle) can be acquired. With the stacked luminescent nanosheets, we achieved the first-ever demonstration of video filming of chronologically changing temperature-shift distribution from the rest state to the active state of the muscles in the living animal. The polymer nanosheet engineering and in vivo microthermography presented in the paper are promising technologies to microscopically explore the heat production and heat transfer in living cells, tissues, and organisms with high spatial resolution beyond what existing thermometric technologies such as infrared thermography have ever achieved. |
| first_indexed | 2024-10-01T06:36:13Z |
| format | Journal Article |
| id | ntu-10356/87221 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:36:13Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/872212023-03-04T17:15:00Z Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals Miyagawa, Takuya Fujie, Toshinori Ferdinandus Vo Doan, Tat Thang Sato, Hirotaka Takeoka, Shinji School of Mechanical and Aerospace Engineering Heat Production Luminescent In this paper, a microthermograph, temperature mapping with high spatial resolution, was established using luminescent molecules embedded ultrathin polymeric films (nanosheets), and demonstrated in a living small animal to map out and visualize temperature shift due to animal’s muscular activity. Herein, we report super flexible and self-adhesive (no need of glue) nanothermosensor consisting of stacked two different polymeric nanosheets with thermosensitive (Eu-tris (dinaphthoylmethane)-bis-trioctylphosphine oxide: EuDT) and insensitive (Rhodamine 800) dyes being embedded. Such stacked nanosheets allow for the ratiometric thermometry, with which the undesired luminescence intensity shift due to focal drift or animal’s z-axis displacement is eliminated and the desired intensity shift solely due to the temperature shift of the sample (living muscle) can be acquired. With the stacked luminescent nanosheets, we achieved the first-ever demonstration of video filming of chronologically changing temperature-shift distribution from the rest state to the active state of the muscles in the living animal. The polymer nanosheet engineering and in vivo microthermography presented in the paper are promising technologies to microscopically explore the heat production and heat transfer in living cells, tissues, and organisms with high spatial resolution beyond what existing thermometric technologies such as infrared thermography have ever achieved. ASTAR (Agency for Sci., Tech. and Research, S’pore) MOE (Min. of Education, S’pore) Published version 2018-02-02T03:49:21Z 2019-12-06T16:37:32Z 2018-02-02T03:49:21Z 2019-12-06T16:37:32Z 2016 Journal Article Miyagawa, T., Fujie, T., Ferdinandus, Vo Doan, T. T., Sato, H., & Takeoka, S. (2016). Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals. ACS Applied Materials & Interfaces, 8(49), 33377-33385. 1944-8244 https://hdl.handle.net/10356/87221 http://hdl.handle.net/10220/44381 10.1021/acsami.6b06075 en ACS Applied Materials & Interfaces © 2016 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. 9 p. application/pdf |
| spellingShingle | Heat Production Luminescent Miyagawa, Takuya Fujie, Toshinori Ferdinandus Vo Doan, Tat Thang Sato, Hirotaka Takeoka, Shinji Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title | Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title_full | Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title_fullStr | Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title_full_unstemmed | Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title_short | Glue-Free Stacked Luminescent Nanosheets Enable High-Resolution Ratiometric Temperature Mapping in Living Small Animals |
| title_sort | glue free stacked luminescent nanosheets enable high resolution ratiometric temperature mapping in living small animals |
| topic | Heat Production Luminescent |
| url | https://hdl.handle.net/10356/87221 http://hdl.handle.net/10220/44381 |
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