Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae
In this study, a biomembrane surface fermentation was used to produce red pigments of Penicillium novae-zelandiae, and the significant improvement in pigment production by the addition of 0.4 g/L of tyrosine demonstrated that the red pigments probably contained betalain. Therefore, one red pigment w...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2018
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| Online Access: | https://hdl.handle.net/10356/87227 http://hdl.handle.net/10220/44355 |
| _version_ | 1826116432261283840 |
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| author | Wang, Hailei Li, Yi Zhang, Kun Ma, Yingqun Li, Ping |
| author2 | Nanyang Environment and Water Research Institute |
| author_facet | Nanyang Environment and Water Research Institute Wang, Hailei Li, Yi Zhang, Kun Ma, Yingqun Li, Ping |
| author_sort | Wang, Hailei |
| collection | NTU |
| description | In this study, a biomembrane surface fermentation was used to produce red pigments of Penicillium novae-zelandiae, and the significant improvement in pigment production by the addition of 0.4 g/L of tyrosine demonstrated that the red pigments probably contained betalain. Therefore, one red pigment was purified, and identified as 2-decarboxybetanin by high-resolution mass spectrometry (MS) and MS/MS analysis. Transcriptomic analysis revealed the differentially expressed genes and metabolic profile of P. novae-zelandiae in response to different cultivations and exhibited the complete biosynthetic pathway of 2-decarboxybetanin in P. novae-zelandiae. Betalains are important water-soluble nitrogen-containing food coloring agents, obtained mainly from beetroot by chemical extraction. This paper is the first report about the production of betalain by microbial fermentation, and results exhibit the possible use of fungal fermentation in future 2-decarboxybetanin production. |
| first_indexed | 2024-10-01T04:11:17Z |
| format | Journal Article |
| id | ntu-10356/87227 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:11:17Z |
| publishDate | 2018 |
| record_format | dspace |
| spelling | ntu-10356/872272020-09-26T21:59:05Z Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae Wang, Hailei Li, Yi Zhang, Kun Ma, Yingqun Li, Ping Nanyang Environment and Water Research Institute Advanced Environmental Biotechnology Centre (AEBC) Betalains Biomembrane Surface Cultivation In this study, a biomembrane surface fermentation was used to produce red pigments of Penicillium novae-zelandiae, and the significant improvement in pigment production by the addition of 0.4 g/L of tyrosine demonstrated that the red pigments probably contained betalain. Therefore, one red pigment was purified, and identified as 2-decarboxybetanin by high-resolution mass spectrometry (MS) and MS/MS analysis. Transcriptomic analysis revealed the differentially expressed genes and metabolic profile of P. novae-zelandiae in response to different cultivations and exhibited the complete biosynthetic pathway of 2-decarboxybetanin in P. novae-zelandiae. Betalains are important water-soluble nitrogen-containing food coloring agents, obtained mainly from beetroot by chemical extraction. This paper is the first report about the production of betalain by microbial fermentation, and results exhibit the possible use of fungal fermentation in future 2-decarboxybetanin production. Published version 2018-01-29T06:12:06Z 2019-12-06T16:37:40Z 2018-01-29T06:12:06Z 2019-12-06T16:37:40Z 2018 Journal Article Wang, H., Li, Y., Zhang, K., Ma, Y., & Li, P. (2018). Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae. AMB Express, 8, 4-. https://hdl.handle.net/10356/87227 http://hdl.handle.net/10220/44355 10.1186/s13568-017-0529-4 en AMB Express © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. 10 p. application/pdf |
| spellingShingle | Betalains Biomembrane Surface Cultivation Wang, Hailei Li, Yi Zhang, Kun Ma, Yingqun Li, Ping Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title | Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title_full | Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title_fullStr | Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title_full_unstemmed | Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title_short | Feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of Penicillium novae-zelandiae |
| title_sort | feasibility and transcriptomic analysis of betalain production by biomembrane surface fermentation of penicillium novae zelandiae |
| topic | Betalains Biomembrane Surface Cultivation |
| url | https://hdl.handle.net/10356/87227 http://hdl.handle.net/10220/44355 |
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